Reviewed, UniProtKB/Swiss-Prot Q1WT04 (GUAC_LACS1)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	LSL_1139

Organism

Lactobacillus salivarius subsp. salivarius (strain UCC118) [Complete proteome] [HAMAP]

Taxonomic identifier

362948 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: InterPro purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	GMP reductase		PRO_0000292053
Regions
	Nucleotide binding	203 – 226	24	NADP Potential
Sites
	Active site	174	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1WT04-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 253C67F02267DE36	FASTA	325	35,714
	10 20 30 40 50 60 MPVFDYEDIQ LVPNKCIVKS RSEVNTKVKF GPMTFKIPVV PANMQTIIDE NLAVWLAKNG 70 80 90 100 110 120 YFYIMHRFYE NERVDFVKNM HDKGLFASIS VGVKPAEYDL IDELSQKNLV PEYITIDIAH 130 140 150 160 170 180 GHSDTVINMI KHIKHKLPGV FVIAGNVGTP EAVRELENAG ADATKVGIGP GKACITKLKT 190 200 210 220 230 240 GFGTGGWQLA AIRACAKAAS KPIVADGGIR NNGDIAKSIR FGASMCMIGS LFAGHDETPG 250 260 270 280 290 300 DIIEKDGKKF KTYFGSASQY QKGEYKNVEG KKLLLPYKGK IADTLREMQE DLQSAISYAG 310 320 GKELLALRKV DYVIVKNSIY NGDML

References

[1]

"Multireplicon genome architecture of Lactobacillus salivarius." Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P., Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K., Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W. Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723(2006) [PubMed: 16617113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000233 Genomic DNA. Translation: ABD99947.1.
RefSeq	YP_536030.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3977879.
GenomeReviews	Gene locus LSL_1139 in contig CP000233_GR.
KEGG	lsl:LSL_1139.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1WT04.
Enzyme and pathway databases
BioCyc	LSAL362948:LSL_1139-MON.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DHase_GMPRtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACS1

Accession

Primary (citable) accession number: Q1WT04

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	May 2, 2006
Last modified:	November 25, 2008
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)