ID   NADE_LACS1              Reviewed;         275 AA.
AC   Q1WSR2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=NH(3)-dependent NAD(+) synthetase;
DE            EC=6.3.1.5;
GN   Name=nadE; OrderedLocusNames=LSL_1260;
OS   Lactobacillus salivarius subsp. salivarius (strain UCC118).
OC   Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=362948;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16617113; DOI=10.1073/pnas.0511060103;
RA   Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P.,
RA   Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C.,
RA   Collins J.K., Higgins D., Shanahan F., Fitzgerald G.F.,
RA   van Sinderen D., O'Toole P.W.;
RT   "Multireplicon genome architecture of Lactobacillus salivarius.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723(2006).
CC   -!- CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP +
CC       diphosphate + NAD(+).
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC       deamido-NAD(+) (ammonia route): step 1/1.
CC   -!- SIMILARITY: Belongs to the NAD synthetase family.
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DR   EMBL; CP000233; ABE00067.1; -; Genomic_DNA.
DR   RefSeq; YP_536150.1; -.
DR   SMR; Q1WSR2; 4-275.
DR   GeneID; 3977293; -.
DR   GenomeReviews; CP000233_GR; LSL_1260.
DR   KEGG; lsl:LSL_1260; -.
DR   HOGENOM; Q1WSR2; -.
DR   OMA; Q1WSR2; ADLEEDR.
DR   BioCyc; LSAL362948:LSL_1260-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0008795; F:NAD+ synthase activity; IEA:EC.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00193; -; 1.
DR   InterPro; IPR003694; NAD_synthase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF02540; NAD_synthase; 1.
DR   TIGRFAMs; TIGR00552; nadE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding.
FT   CHAIN         1    275       NH(3)-dependent NAD(+) synthetase.
FT                                /FTId=PRO_1000077570.
FT   NP_BIND      47     54       ATP (By similarity).
FT   ACT_SITE     49     49       By similarity.
SQ   SEQUENCE   275 AA;  30919 MW;  4F59D2E5A3143D43 CRC64;
     MRPLQERIIN ELKVKPEINA EEEIRRSVDF LKDYLKKHPF LKALVLGISG GQDSTLAGKL
     SQMAISELRE ETGDDAYQFI AVRLPYGDQA DEQDAMDAIE FMQADKTVRV NIKQAADAMV
     QSIEENGLEI SDFNKGNIKA RERMIAQYGI AGAVSGAVVG TDHAAEAITG FYTKYGDGGA
     DITPLWRLDK RQGRAMLELL NAPEHLYKKV PTADLEEDRP ALPDEVALGV TYNDIDDYLE
     GKEVADNVAE KIENWFLKTE HKRHMPINVY DTFWK
//