Q1WSR2 (NADE_LACS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	LSL_1260

Organism

Lactobacillus salivarius (strain UCC118) [Complete proteome] [HAMAP]

Taxonomic identifier

362948 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077570
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity
Sites
	Active site	49	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1WSR2 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 4F59D2E5A3143D43	FASTA	275	30,919
	10 20 30 40 50 60 MRPLQERIIN ELKVKPEINA EEEIRRSVDF LKDYLKKHPF LKALVLGISG GQDSTLAGKL 70 80 90 100 110 120 SQMAISELRE ETGDDAYQFI AVRLPYGDQA DEQDAMDAIE FMQADKTVRV NIKQAADAMV 130 140 150 160 170 180 QSIEENGLEI SDFNKGNIKA RERMIAQYGI AGAVSGAVVG TDHAAEAITG FYTKYGDGGA 190 200 210 220 230 240 DITPLWRLDK RQGRAMLELL NAPEHLYKKV PTADLEEDRP ALPDEVALGV TYNDIDDYLE 250 260 270 GKEVADNVAE KIENWFLKTE HKRHMPINVY DTFWK

References

[1]

"Multireplicon genome architecture of Lactobacillus salivarius." Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P., Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K., Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W. Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723(2006) [PubMed: 16617113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UCC118.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000233 Genomic DNA. Translation: ABE00067.1.
RefSeq	YP_536150.1. NC_007929.1.
3D structure databases
ProteinModelPortal	Q1WSR2.
SMR	Q1WSR2. Positions 4-275.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1WSR2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3977293.
GenomeReviews	Gene locus LSL_1260 in contig CP000233_GR.
KEGG	lsl:LSL_1260.
PATRIC	22279431. VBILacSal43464_1334.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	IAQYEIA.
PhylomeDB	Q1WSR2.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_LACS1

Accession

Primary (citable) accession number: Q1WSR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 2, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program