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Q1WSM8 (GLGB_LACS1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:LSL_1294
OrganismLactobacillus salivarius (strain UCC118) [Complete proteome] [HAMAP]
Taxonomic identifier362948 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length649 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6496491,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260663

Sites

Active site3151Nucleophile By similarity
Active site3661Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1WSM8 [UniParc].

Last modified May 2, 2006. Version 1.
Checksum: 3C1AC6475F0C4921

FASTA64976,186
        10         20         30         40         50         60 
MAVNTEERHG QESLENHKHM FNQGVNYELY NFLGVHKISR DDESGYVFRV WAPHAKQVWV 

        70         80         90        100        110        120 
CGDFNNWDKS HPMILDEKSG IWSIEVKESR ENQYYKYLVK QANGREIMKT DPMAQVYEKR 

       130        140        150        160        170        180 
PETAAVVKEL EPFKWNDGLW RGRQKRMNHF NNPINIYEVH APSWKEHEDG SLYTFKDLTA 

       190        200        210        220        230        240 
ELIPYVKKMG YTHIEFMPLM EHPLPASWGY QLTGYFALCS SYGTPDEFKD FVNECHQNNI 

       250        260        270        280        290        300 
GVLVDWVPGH FSINDDALAY YDGTPTYEYE DPDRAKNIGW GALNFDLGKP EVQSFLLSSA 

       310        320        330        340        350        360 
FYWLNEFHLD GMRVDAVSNM IYLDYDEGPW KPNKYGDTRN LEGYAFLQKF NKVVKFAHPE 

       370        380        390        400        410        420 
SLIIAEESSS GTQISGTIES GAIGFDYKWN MGWMNDVLEF FEMDPYFRKD NLNLVTFSFM 

       430        440        450        460        470        480 
YWQAENFVLP LSHDEVVHGK KSLMHKMWGD RYRQFAQLRT LYTFMMMHPG KKLLFMSGEW 

       490        500        510        520        530        540 
GQFLEWKFDH GLEWVDLQDE MNAKMQHFTS VLNHFYKEER PLWELGQQDA TLEVIDADNH 

       550        560        570        580        590        600 
NDTVLSFIRH GKRKKDFLIV ILNFTPVERR NFRIGVPYEG TYTEILNTEM KEFGGTWVEH 

       610        620        630        640 
NADSVSEEVN FKDYEHSITT TVPALGAIIL KPKDIKVTRR SSKKHSHKK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000233 Genomic DNA. Translation: ABE00101.1.
RefSeqYP_536184.1. NC_007929.1.

3D structure databases

ProteinModelPortalQ1WSM8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING362948.LSL_1294.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE00101; ABE00101; LSL_1294.
GeneID3976546.
KEGGlsl:LSL_1294.
PATRIC22279511. VBILacSal43464_1368.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAECHQNNI.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycLSAL362948:GJDJ-1382-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_LACS1
AccessionPrimary (citable) accession number: Q1WSM8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: May 2, 2006
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries