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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Lactobacillus salivarius (strain UCC118)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei77Charge relay systemUniRule annotation1
Active sitei152Charge relay systemUniRule annotation1
Active sitei176Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:LSL_1345
OrganismiLactobacillus salivarius (strain UCC118)
Taxonomic identifieri362948 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000006559 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000158501 – 487Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST487

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi362948.LSL_1345.

Structurei

3D structure databases

ProteinModelPortaliQ1WSH9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiHASPLEM.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1WSH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYFKNDLAS IHDDLVNKKY SVEELTKSTF DNIKKVDKEI EAFLKLDEER
60 70 80 90 100
ALEKARKIDE RGVKADSILD GIGIGIKDNI VTKDLTTTAA SKMLENFEPI
110 120 130 140 150
YNATVMDKLN DAGMITVGKL NMDEFAMGSS TENSAFKITK NVWDTTKVPG
160 170 180 190 200
GSSGGSAAAV ASGQVLASLG SDTGGSIRQP AAFNGIVGVK PTYGRVSRYG
210 220 230 240 250
LIAFASSLDQ IGPLTRTVKD NALVLNAISG HDAHDFTSSS REVPDFTKGI
260 270 280 290 300
EDGVKGMRIA VPKEYFGEGV DEDVAKAVKD AVKTFESLGA TVDEISLPHS
310 320 330 340 350
KYGVPVYYII ASSEASSNLQ RYDGIRYGFR AKDVKNLEDV YVRSRSEGFG
360 370 380 390 400
DEVKRRIMLG TFSLSAGSYD AFFKKAAQVR TLICQDFENV YKDYDLILAP
410 420 430 440 450
TSPTTAYGIG EEINDPVTMY MNDILTIPVN LAGLPGMSLP AGFSNGLPIG
460 470 480
LQLIAPRFAE STMYRAGYAF EQSTDFHKAI PTLGGQN
Length:487
Mass (Da):52,778
Last modified:May 2, 2006 - v1
Checksum:iF437B133A6BAE302
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000233 Genomic DNA. Translation: ABE00150.1.
RefSeqiWP_011476296.1. NC_007929.1.
YP_536233.1. NC_007929.1.

Genome annotation databases

EnsemblBacteriaiABE00150; ABE00150; LSL_1345.
GeneIDi3976577.
KEGGilsl:LSL_1345.
PATRICi22279615. VBILacSal43464_1420.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000233 Genomic DNA. Translation: ABE00150.1.
RefSeqiWP_011476296.1. NC_007929.1.
YP_536233.1. NC_007929.1.

3D structure databases

ProteinModelPortaliQ1WSH9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi362948.LSL_1345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE00150; ABE00150; LSL_1345.
GeneIDi3976577.
KEGGilsl:LSL_1345.
PATRICi22279615. VBILacSal43464_1420.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiHASPLEM.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_LACS1
AccessioniPrimary (citable) accession number: Q1WSH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 2, 2006
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.