Q1WNZ9 (PB2_I77AB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polymerase basic protein 2

Alternative name(s):
RNA-directed RNA polymerase subunit P3

Gene names

Name:

PB2

Organism

Influenza A virus (strain A/USSR/90/1977 H1N1) [Complete proteome]

Taxonomic identifier

381516 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication By similarity.
Subunit structure	Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.
Subcellular location	Virion. Host nucleus. Host mitochondrion By similarity.
Sequence similarities	Belongs to the influenza viruses PB2 family.

Ontologies

Keywords
Biological process	Cap snatching Host gene expression shutoff by virus Host transcription shutoff by virus Host-virus interaction Inhibition of host RNA polymerase II by virus mRNA capping mRNA processing
Cellular component	Host mitochondrion Host nucleus Virion
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	7-methylguanosine mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host RNA polymerase II activity Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Polymerase basic protein 2		PRO_0000279651
Regions
	Motif	736 – 739	4	Nuclear localization signal By similarity
Experimental info
	Sequence conflict	469	1	S → P in ABF21235. Ref.2
	Sequence conflict	759	1	N → I in ABF21235. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q1WNZ9 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 8646B1D78D3EF02E	FASTA	759	86,001
	10 20 30 40 50 60 MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPSLRMKWM MAMKYPITAD 70 80 90 100 110 120 KRITEMIPER NEQGQTLWSK MNDAGSDRVM VSPLAVTWWN RNGPVTSTVH YPKIYKTYFE 130 140 150 160 170 180 KVERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE 190 200 210 220 230 240 SQLTITKEKK EELQNCKISP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW 250 260 270 280 290 300 EQMYTPGGEV RNDDVDQSLI IAARNIVRRA AVSADPLASL LEMCHSTQIG GTRMVDILRQ 310 320 330 340 350 360 NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ TLKIKVHEGY 370 380 390 400 410 420 EEFTMVGKRA TAILRKATRR LIQLIVSGRD EQSIVEAIVV AMVFSQEDCM IKAVRGDLNF 430 440 450 460 470 480 VNRANQRLNP MHQLLRHFQK DAKVLFQNWG IEPIDNVMGM IGILPDMTSS TEMSMRGVRV 490 500 510 520 530 540 SKMGVDEYSN AERVVVSIDR FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN 550 560 570 580 590 600 GPESVLVNTY QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAIRG QYSGFVRTLF 610 620 630 640 650 660 QQMRDVLGTF DTTQIIKLLP FAAAPPKQSR MQFSSLTVNV RGSGMRILVR GNSPVFNYNK 670 680 690 700 710 720 TTKRLTVLGK DAGTLTEDPD EGTAGVESAV LRGFLILGKE DRRYGPALSI NELSNLAKGE 730 740 750 KANVLIGQGD VVLVMKRKRD SSILTDSQTA TKRIRMAIN

References

[1]

"The NIAID influenza genome sequencing project." Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V., Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H., Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y. , Bolotov P., Dernovoy D., Kiryutin B., Lipman D.J., Tatusova T. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

[2]

"Complete genome sequencing and analysis of selected influenza virus vaccine strains spanning six decades (1933-1999)." Mbawuike I.N., Zhang Y., Yamada R.E., Nino D., Bui H.-H., Sette A., Couch R.B. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CY010379 Genomic RNA. Translation: ABD95360.1. DQ508894 Genomic RNA. Translation: ABF21235.1.
3D structure databases
ProteinModelPortal	Q1WNZ9.
SMR	Q1WNZ9. Positions 319-483, 685-759.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001591. RNA_pol_PB2_orthomyxovir. [Graphical view]
Pfam	PF00604. Flu_PB2. 1 hit. [Graphical view]
ProDom	PD001667. RNA_pol_PB2_orthomyxovir. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PB2_I77AB

Accession

Primary (citable) accession number: Q1WNZ9
Secondary accession number(s): Q1K9I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	May 2, 2006
Last modified:	January 9, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program