Reviewed, UniProtKB/Swiss-Prot Q1WM15 (HXK8_ORYSJ)

Names and origin

Protein names

Recommended name:
    Hexokinase-8
    EC=2.7.1.1
Alternative name(s):
    Hexokinase-4

Gene names

Name:	HXK8
Synonyms:	HXK4
Ordered Locus Names:	Os01g0190400, LOC_Os01g09460
ORF Names:	P0710E05.19

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. Ref.1
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Tissue specificity	Expressed in roots, leaves, flowers, immature seeds, endosperm and seed coat. Ref.1
Developmental stage	Expressed during flower development until 15 days after flowering. Ref.1
Induction	Not induced by glucose or fructose treatment in leaves. Ref.1
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Hexokinase-8		PRO_0000247571
Regions
	Nucleotide binding	70 – 75	6	ATP Potential
	Region	140 – 166	27	Glucose-binding Potential
Experimental info
	Sequence conflict	20	1	P → R in AAZ93625. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q1WM15-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: 1FA09220E566F7DF	FASTA	458	49,379
	10 20 30 40 50 60 MAAVEAEKVV AELRERCATP ASLLRDVAAA MAGEMGAGLE KEGGSRVKML LSYVDKLPTG 70 80 90 100 110 120 REDGLFYGLD LGGTNFRVLK VHLGGSKKHV VNSESREVSI PPHLMSGTSS ELFGFIAGEL 130 140 150 160 170 180 GKFVAEEEEG TDMPNGKKKE LGFTFSFPVR QRSVASGTLV KWTKAFSIDD AVGEDVVAEL 190 200 210 220 230 240 QTAMVKQGLD MHVAALINDA VGTLAGARYY DEDVVAGVIF GTGTNAAYVE KANAIPKWEG 250 260 270 280 290 300 ELPNSGDMVI NMEWGNFYSS HLPVTEYDEA LDKESLNPGE QIYEKLTSGM YLGEIVRRVL 310 320 330 340 350 360 LKLSLQSGIF GSIDNSKLKT CFHLRTPHIS AMHHDETPDL KIVAEKLHQI LEITHTSLEI 370 380 390 400 410 420 RKMVVEICDI VARRAARLAA AGVAGILMKL GRNGGINNQR SVIAIDGGLF EHYTKFRECL 430 440 450 ESTLGELLGE EASKSVAVKH ANDGSGIGAA LIAASQSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)." Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H., Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S. Planta 224:598-611(2006) [PubMed: 16552590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, NOMENCLATURE. Strain: cv. Jinmi.
[2]	"The hexokinase gene family in rice." Wang Y.D., Cheng W., Wang X.S., Zhou X.J. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Zhonghua 15. Tissue: Flower.
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ116390 mRNA. Translation: AAZ93625.1. AY884167 mRNA. Translation: AAX68420.1. AP002743 Genomic DNA. Translation: BAD72221.1.
3D structure databases
SMR	Q1WM15. Positions 7-455.
ModBase	Search...
Organism-specific databases
Gramene	Q5SNH9.
Family and domain databases
InterPro	IPR001312. Hexokinase. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK8_ORYSJ

Accession

Primary (citable) accession number: Q1WM15
Secondary accession number(s): Q5SNH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	January 19, 2010
This is version 18 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)