ID   GAPN_ARATH              Reviewed;         496 AA.
AC   Q1WIQ6; Q9ZUG8;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 2.
DT   16-JUN-2009, entry version 26.
DE   RecName: Full=NADP-dependent glyceraldehyde-3-phosphate dehydrogenase;
DE            EC=1.2.1.9;
DE   AltName: Full=Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase;
DE   AltName: Full=Glyceraldehyde-3-phosphate dehydrogenase [NADP+];
DE   AltName: Full=Triosephosphate dehydrogenase;
DE   AltName: Full=Aldehyde dehydrogenase family 11 member A3;
GN   Name=ALDH11A3; Synonyms=GAPN; OrderedLocusNames=At2g24270;
GN   ORFNames=F27D4.18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RA   Rius S.P., Iglesias A.A., Gomez-Casati D.F.;
RL   Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083487; PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
RA   Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
RA   Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
RA   Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
RA   Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
RA   Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NOMENCLATURE.
RX   PubMed=15358267; DOI=10.1016/j.tplants.2004.06.004;
RA   Kirch H.-H., Bartels D., Wei Y., Schnable P.S., Wood A.J.;
RT   "The ALDH gene superfamily of Arabidopsis.";
RL   Trends Plant Sci. 9:371-377(2004).
CC   -!- FUNCTION: Important as a means of generating NADPH for
CC       biosynthetic reactions.
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + NADP(+) + H(2)O
CC       = 3-phospho-D-glycerate + NADPH.
CC   -!- INTERACTION:
CC       Q42403:TRX3; NbExp=1; IntAct=EBI-449197, EBI-449157;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
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DR   EMBL; DQ268762; ABB83822.1; -; mRNA.
DR   EMBL; AC005967; AAD03388.1; -; Genomic_DNA.
DR   EMBL; AY037205; AAK59790.1; -; mRNA.
DR   EMBL; AY136409; AAM97075.1; -; mRNA.
DR   EMBL; BT004551; AAO42797.1; -; mRNA.
DR   IPI; IPI00530233; -.
DR   PIR; F84634; F84634.
DR   RefSeq; NP_180004.1; -.
DR   RefSeq; NP_973526.1; -.
DR   UniGene; At.22354; -.
DR   HSSP; Q59931; 1EUH.
DR   IntAct; Q1WIQ6; 1.
DR   PRIDE; Q1WIQ6; -.
DR   GeneID; 816962; -.
DR   GenomeReviews; CT485783_GR; AT2G24270.
DR   KEGG; ath:AT2G24270; -.
DR   NMPDR; fig|3702.1.peg.9449; -.
DR   TAIR; At2g24270; -.
DR   OMA; Q1WIQ6; SRPKATV.
DR   BioCyc; MetaCyc:AT2G24270-MON; -.
DR   BRENDA; 1.2.1.9; 302.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008886; F:glyceraldehyde-3-phosphate dehydrogenase (N...; IDA:TAIR.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR016160; Ald_DH_CS.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH.
DR   Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
DR   PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytoplasm; NADP; Oxidoreductase.
FT   CHAIN         1    496       NADP-dependent glyceraldehyde-3-phosphate
FT                                dehydrogenase.
FT                                /FTId=PRO_0000256066.
FT   NP_BIND     245    249       NAD (By similarity).
FT   REGION      169    170       Substrate binding (By similarity).
FT   REGION      297    299       Substrate binding (By similarity).
FT   ACT_SITE    264    264       Proton acceptor (By similarity).
FT   ACT_SITE    298    298       Nucleophile (By similarity).
FT   BINDING     116    116       Substrate (By similarity).
FT   BINDING     192    192       NADP (By similarity).
FT   BINDING     195    195       NADP (By similarity).
FT   BINDING     230    230       NADP (By similarity).
FT   BINDING     391    391       NADP (By similarity).
FT   BINDING     451    451       Substrate (By similarity).
FT   SITE        169    169       Transition state stabilizer (By
FT                                similarity).
FT   CONFLICT     47     47       V -> A (in Ref. 1; ABB83822).
FT   CONFLICT     56     56       N -> Y (in Ref. 1; ABB83822).
SQ   SEQUENCE   496 AA;  53060 MW;  9231656A951175D5 CRC64;
     MAGTGLFAEI LDGEVYKYYA DGEWKTSSSG KSVAIMNPAT RKTQYKVQAC TQEEVNAVME
     LAKSAQKSWA KTPLWKRAEL LHKAAAILKD NKAPMAESLV KEIAKPAKDS VTEVVRSGDL
     ISYCAEEGVR ILGEGKFLLS DSFPGNDRTK YCLTSKIPLG VVLAIPPFNY PVNLAVSKIA
     PALIAGNSLV LKPPTQGAVS CLHMVHCFHL AGFPKGLISC ITGKGSEIGD FLTMHPAVNC
     ISFTGGDTGI SISKKAGMIP LQMELGGKDA CIVLDDADLD LVASNIIKGG FSYSGQRCTA
     VKVVLVMESV ADELVEKVKA KVAKLTVGPP EENSDITAVV SESSANFIEG LVMDAKEKGA
     TFCQEYKREG NLIWPLLLDN VRPDMRIAWE EPFGPVVPVL RINSVEEGIN HCNASNFGLQ
     GCVFTKDINK AILISDAMET GTVQINSAPA RGPDHFPFQG LKDSGIGSQG VTNSINLMTK
     VKTTVINLPT PSYSMG
//