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Protein

NADP-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene

ALDH11A3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Important as a means of generating NADPH for biosynthetic reactions.

Catalytic activityi

D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161SubstrateBy similarity
Sitei169 – 1691Transition state stabilizerBy similarity
Binding sitei192 – 1921NADPBy similarity
Binding sitei195 – 1951NADPBy similarity
Binding sitei230 – 2301NADPBy similarity
Active sitei264 – 2641Proton acceptorPROSITE-ProRule annotation
Active sitei298 – 2981NucleophilePROSITE-ProRule annotation
Binding sitei391 – 3911NADPBy similarity
Binding sitei451 – 4511SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi245 – 2495NADBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT2G24270-MONOMER.
ARA:GQT-2322-MONOMER.
ARA:GQT-2324-MONOMER.
ARA:GQT-2398-MONOMER.
MetaCyc:AT2G24270-MONOMER.
ReactomeiR-ATH-70688. Proline catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.9)
Alternative name(s):
Aldehyde dehydrogenase family 11 member A3
Glyceraldehyde-3-phosphate dehydrogenase [NADP(+)]
Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase
Triosephosphate dehydrogenase
Gene namesi
Name:ALDH11A3
Synonyms:GAPN
Ordered Locus Names:At2g24270
ORF Names:F27D4.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G24270.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 496495NADP-dependent glyceraldehyde-3-phosphate dehydrogenasePRO_0000256066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei4 – 41PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ1WIQ6.
PRIDEiQ1WIQ6.

PTM databases

iPTMnetiQ1WIQ6.

Expressioni

Gene expression databases

ExpressionAtlasiQ1WIQ6. baseline and differential.
GenevisibleiQ1WIQ6. AT.

Interactioni

Protein-protein interaction databases

BioGridi2314. 4 interactions.
IntActiQ1WIQ6. 1 interaction.
STRINGi3702.AT2G24270.4.

Structurei

3D structure databases

ProteinModelPortaliQ1WIQ6.
SMRiQ1WIQ6. Positions 16-488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni169 – 1702Substrate bindingBy similarity
Regioni297 – 2993Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiKOG2450. Eukaryota.
COG1012. LUCA.
HOGENOMiHOG000271509.
InParanoidiQ1WIQ6.
KOiK00131.
PhylomeDBiQ1WIQ6.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q1WIQ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGTGLFAEI LDGEVYKYYA DGEWKTSSSG KSVAIMNPAT RKTQYKVQAC
60 70 80 90 100
TQEEVNAVME LAKSAQKSWA KTPLWKRAEL LHKAAAILKD NKAPMAESLV
110 120 130 140 150
KEIAKPAKDS VTEVVRSGDL ISYCAEEGVR ILGEGKFLLS DSFPGNDRTK
160 170 180 190 200
YCLTSKIPLG VVLAIPPFNY PVNLAVSKIA PALIAGNSLV LKPPTQGAVS
210 220 230 240 250
CLHMVHCFHL AGFPKGLISC ITGKGSEIGD FLTMHPAVNC ISFTGGDTGI
260 270 280 290 300
SISKKAGMIP LQMELGGKDA CIVLDDADLD LVASNIIKGG FSYSGQRCTA
310 320 330 340 350
VKVVLVMESV ADELVEKVKA KVAKLTVGPP EENSDITAVV SESSANFIEG
360 370 380 390 400
LVMDAKEKGA TFCQEYKREG NLIWPLLLDN VRPDMRIAWE EPFGPVVPVL
410 420 430 440 450
RINSVEEGIN HCNASNFGLQ GCVFTKDINK AILISDAMET GTVQINSAPA
460 470 480 490
RGPDHFPFQG LKDSGIGSQG VTNSINLMTK VKTTVINLPT PSYSMG
Length:496
Mass (Da):53,060
Last modified:October 31, 2006 - v2
Checksum:i9231656A951175D5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471V → A in ABB83822 (Ref. 1) Curated
Sequence conflicti56 – 561N → Y in ABB83822 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ268762 mRNA. Translation: ABB83822.1.
AC005967 Genomic DNA. Translation: AAD03388.1.
CP002685 Genomic DNA. Translation: AEC07552.1.
CP002685 Genomic DNA. Translation: AEC07553.1.
CP002685 Genomic DNA. Translation: AEC07554.1.
AY037205 mRNA. Translation: AAK59790.1.
AY136409 mRNA. Translation: AAM97075.1.
BT004551 mRNA. Translation: AAO42797.1.
PIRiF84634.
RefSeqiNP_001189589.1. NM_001202660.1. [Q1WIQ6-1]
NP_180004.1. NM_127989.3. [Q1WIQ6-1]
NP_973526.1. NM_201797.3. [Q1WIQ6-1]
UniGeneiAt.22354.

Genome annotation databases

EnsemblPlantsiAT2G24270.1; AT2G24270.1; AT2G24270. [Q1WIQ6-1]
AT2G24270.2; AT2G24270.2; AT2G24270. [Q1WIQ6-1]
AT2G24270.3; AT2G24270.3; AT2G24270. [Q1WIQ6-1]
GeneIDi816962.
KEGGiath:AT2G24270.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ268762 mRNA. Translation: ABB83822.1.
AC005967 Genomic DNA. Translation: AAD03388.1.
CP002685 Genomic DNA. Translation: AEC07552.1.
CP002685 Genomic DNA. Translation: AEC07553.1.
CP002685 Genomic DNA. Translation: AEC07554.1.
AY037205 mRNA. Translation: AAK59790.1.
AY136409 mRNA. Translation: AAM97075.1.
BT004551 mRNA. Translation: AAO42797.1.
PIRiF84634.
RefSeqiNP_001189589.1. NM_001202660.1. [Q1WIQ6-1]
NP_180004.1. NM_127989.3. [Q1WIQ6-1]
NP_973526.1. NM_201797.3. [Q1WIQ6-1]
UniGeneiAt.22354.

3D structure databases

ProteinModelPortaliQ1WIQ6.
SMRiQ1WIQ6. Positions 16-488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2314. 4 interactions.
IntActiQ1WIQ6. 1 interaction.
STRINGi3702.AT2G24270.4.

PTM databases

iPTMnetiQ1WIQ6.

Proteomic databases

PaxDbiQ1WIQ6.
PRIDEiQ1WIQ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G24270.1; AT2G24270.1; AT2G24270. [Q1WIQ6-1]
AT2G24270.2; AT2G24270.2; AT2G24270. [Q1WIQ6-1]
AT2G24270.3; AT2G24270.3; AT2G24270. [Q1WIQ6-1]
GeneIDi816962.
KEGGiath:AT2G24270.

Organism-specific databases

TAIRiAT2G24270.

Phylogenomic databases

eggNOGiKOG2450. Eukaryota.
COG1012. LUCA.
HOGENOMiHOG000271509.
InParanoidiQ1WIQ6.
KOiK00131.
PhylomeDBiQ1WIQ6.

Enzyme and pathway databases

BioCyciARA:AT2G24270-MONOMER.
ARA:GQT-2322-MONOMER.
ARA:GQT-2324-MONOMER.
ARA:GQT-2398-MONOMER.
MetaCyc:AT2G24270-MONOMER.
ReactomeiR-ATH-70688. Proline catabolism.

Miscellaneous databases

PROiQ1WIQ6.

Gene expression databases

ExpressionAtlasiQ1WIQ6. baseline and differential.
GenevisibleiQ1WIQ6. AT.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Rius S.P., Iglesias A.A., Gomez-Casati D.F.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. Cited for: NOMENCLATURE.
  6. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-4, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGAPN_ARATH
AccessioniPrimary (citable) accession number: Q1WIQ6
Secondary accession number(s): Q9ZUG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: January 20, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.