Reviewed, UniProtKB/Swiss-Prot Q1WIQ6 (GAPN_ARATH)

Names and origin

Protein names

Recommended name:
    NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
    EC=1.2.1.9
Alternative name(s):
    Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase
    Glyceraldehyde-3-phosphate dehydrogenase [NADP+]
    Triosephosphate dehydrogenase
    Aldehyde dehydrogenase family 11 member A3

Gene names

Name:	ALDH11A3
Synonyms:	GAPN
Ordered Locus Names:	At2g24270
ORF Names:	F27D4.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important as a means of generating NADPH for biosynthetic reactions.
Catalytic activity	D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) activity Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449197,EBI-449157

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	NADP-dependent glyceraldehyde-3-phosphate dehydrogenase		PRO_0000256066
Regions
	Nucleotide binding	245 – 249	5	NAD By similarity
	Region	169 – 170	2	Substrate binding By similarity
	Region	297 – 299	3	Substrate binding By similarity
Sites
	Active site	264	1	Proton acceptor By similarity
	Active site	298	1	Nucleophile By similarity
	Binding site	116	1	Substrate By similarity
	Binding site	192	1	NADP By similarity
	Binding site	195	1	NADP By similarity
	Binding site	230	1	NADP By similarity
	Binding site	391	1	NADP By similarity
	Binding site	451	1	Substrate By similarity
	Site	169	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	47	1	V → A in ABB83822. Ref.1
	Sequence conflict	56	1	N → Y in ABB83822. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q1WIQ6-1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 9231656A951175D5	FASTA	496	53,060
	10 20 30 40 50 60 MAGTGLFAEI LDGEVYKYYA DGEWKTSSSG KSVAIMNPAT RKTQYKVQAC TQEEVNAVME 70 80 90 100 110 120 LAKSAQKSWA KTPLWKRAEL LHKAAAILKD NKAPMAESLV KEIAKPAKDS VTEVVRSGDL 130 140 150 160 170 180 ISYCAEEGVR ILGEGKFLLS DSFPGNDRTK YCLTSKIPLG VVLAIPPFNY PVNLAVSKIA 190 200 210 220 230 240 PALIAGNSLV LKPPTQGAVS CLHMVHCFHL AGFPKGLISC ITGKGSEIGD FLTMHPAVNC 250 260 270 280 290 300 ISFTGGDTGI SISKKAGMIP LQMELGGKDA CIVLDDADLD LVASNIIKGG FSYSGQRCTA 310 320 330 340 350 360 VKVVLVMESV ADELVEKVKA KVAKLTVGPP EENSDITAVV SESSANFIEG LVMDAKEKGA 370 380 390 400 410 420 TFCQEYKREG NLIWPLLLDN VRPDMRIAWE EPFGPVVPVL RINSVEEGIN HCNASNFGLQ 430 440 450 460 470 480 GCVFTKDINK AILISDAMET GTVQINSAPA RGPDHFPFQG LKDSGIGSQG VTNSINLMTK 490 VKTTVINLPT PSYSMG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Rius S.P., Iglesias A.A., Gomez-Casati D.F. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The ALDH gene superfamily of Arabidopsis." Kirch H.-H., Bartels D., Wei Y., Schnable P.S., Wood A.J. Trends Plant Sci. 9:371-377(2004) [PubMed: 15358267] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ268762 mRNA. Translation: ABB83822.1. AC005967 Genomic DNA. Translation: AAD03388.1. AY037205 mRNA. Translation: AAK59790.1. AY136409 mRNA. Translation: AAM97075.1. BT004551 mRNA. Translation: AAO42797.1.
IPI	IPI00530233.
PIR	F84634.
RefSeq	NP_180004.1. NP_973526.1.
UniGene	At.22354
3D structure databases
HSSP	HSSP built from PDB template 1EUH based on UniProtKB Q59931.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q1WIQ6. 1 interaction.
Proteomic databases
PRIDE	Q1WIQ6.
Genome annotation databases
GeneID	816962.
GenomeReviews	Gene locus AT2G24270 in contig CT485783_GR.
KEGG	ath:AT2G24270.
NMPDR	fig|3702.1.peg.9449.
Organism-specific databases
TAIR	At2g24270.
Phylogenomic databases
OMA	Q1WIQ6. SRPKATV.
Enzyme and pathway databases
BioCyc	MetaCyc:AT2G24270-MON.
BRENDA	1.2.1.9. 302.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAPN_ARATH

Accession

Primary (citable) accession number: Q1WIQ6
Secondary accession number(s): Q9ZUG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)