Reviewed, UniProtKB/Swiss-Prot Q1W375 (PMM_TOBAC)

Names and origin

Protein names	Recommended name:     Phosphomannomutase     EC=5.4.2.8 Alternative name(s):     NtPMM
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in ascorbic acid biosynthesis and in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
Pathway	Nucleotide-sugar biosynthesis; GDP-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the eukaryotic PMM family.
Caution	Ref.1 indicates this sequence as being of Nicotiana benthamiana origin.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	mannose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphomannomutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Phosphomannomutase		PRO_0000326496

Sequences

	Sequence		Length	Mass (Da)
	Q1W375-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: E9820AF015B0099A	FASTA	252	28,575
	10 20 30 40 50 60 MAARKPGLIA LFDVDGTLTA PRKEVTPEML KFMKELRKVV TVGVVGGSDL VKISEQLGKT 70 80 90 100 110 120 VTTDYDYCFS ENGLVAHKDG KLIGTQSLKS FLGDEKLKEF INFTLHYIAD LDIPIKRGTF 130 140 150 160 170 180 IEFRSGMLNV SPIGRDCSQE ERDEFEKYDK VHKIRQTMVS VLREKFAHLN LTFSIGGQIS 190 200 210 220 230 240 FDVFPQGWDK TYCLRYLEEF NEIHFFGDKT YKGGNDHEIY ESERTVGHTV TSPEDTVKQC 250 SEQFLGKDNG SS

References

[1]

"Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana." Qian W., Yu C., Qin H., Liu X., Zhang A., Johansen I.E., Wang D. Plant J. 49:399-413(2007) [PubMed: 17217471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ442995 mRNA. Translation: ABD97874.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.4.2.8. 298.
Family and domain databases
InterPro	IPR006379. HAD-SF_hydro_IIB. IPR005002. PMM. [Graphical view]
PANTHER	PTHR10466. PMM. 1 hit.
Pfam	PF03332. PMM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01484. HAD-SF-IIB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PMM_TOBAC

Accession

Primary (citable) accession number: Q1W375

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 2, 2006
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)