Reviewed, UniProtKB/Swiss-Prot Q1W2L8 (GSH1_TOBAC)

Names and origin

Protein names

Recommended name:
    Glutamate--cysteine ligase, chloroplastic
    EC=6.3.2.2
Alternative name(s):
    Gamma-glutamylcysteine synthetase
    Gamma-ECS
      Short name=GCS

Gene names

Name:

GSH1

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Subunit structure	Homodimer or monomer when oxidized or reduced, respectively By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Post-translational modification	The Cys-186-Cys-406 disulfide bridge is known to modulate the enzyme activity according to the redox status. The oxidized form constitutes the active enzyme By similarity.
Sequence similarities	Belongs to the glutamate--cysteine ligase type 2 family.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Chloroplast Potential
	Chain	46 – 522	477	Glutamate--cysteine ligase, chloroplastic		PRO_0000333025
Amino acid modifications
	Disulfide bond	186 ↔ 406		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1W2L8-1 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: 590EBC7DE01FEEDC	FASTA	522	58,962
	10 20 30 40 50 60 MALMSQAGSS HCIYSEKMKC ISGHSSITSN MEMLKMKDIC FGNISSRNSS KPMQGIYLDR 70 80 90 100 110 120 VGVERRRGRL AIVAASPPTE DAVVAAEPLT KEDLVAYLAS GCKSKEKWRI GTEHEKFGFE 130 140 150 160 170 180 FGTLRPMKYE QIAELLNGIA ERFDWEKVME GDNIIGLKQG KQSISLEPGG QFELSGAPLE 190 200 210 220 230 240 TLHQTCAEVN SHLYQVKAVA EEMGIGFLGT GFQPKWGLKD IPVMPKGRYE IMRNYMPKVG 250 260 270 280 290 300 SLGLDMMFRT CTVQVNLDFS SEADMIRKFR AGLALQPIAT ALFANSPFTE GKPNGYLSMR 310 320 330 340 350 360 SHIWTDTDNN RAGMLPFVFD DSFGFEQYVD YALDVPMYFV YRKKKYIDCA GMSFRDFMNG 370 380 390 400 410 420 KLSPIPGDYP TLNDWENHLT TIFPEVRLKR YLEMRGADGG PWRRLCALPA FWVGILYDEV 430 440 450 460 470 480 SLQTVLDMTS DWTAEEREML RNKVPTSGLK TPFRDGLLKH VAQDVVKLAK EGLERRGYKE 490 500 510 520 TGFLNEVTEV VRTGVTPAEK LLELYHGKWG RSVDPVFEEL LY

References

[1]	"Regulation of plant and bacterial gamma-glutamylcysteine synthetase." Gromes R., Rausch T. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ444219 mRNA. Translation: ABD98695.2.
3D structure databases
SMR	Q1W2L8. Positions 87-522.
ModBase	Search...
Family and domain databases
InterPro	IPR006336. GCS2. IPR011556. Glut_cys_lig_pln. [Graphical view]
Pfam	PF04107. GCS2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01436. glu_cys_lig_pln. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_TOBAC

Accession

Primary (citable) accession number: Q1W2L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 18 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)