Reviewed, UniProtKB/Swiss-Prot Q1SGF1 (PARP3_MEDTR)

Names and origin

Protein names

Recommended name:
    Putative poly [ADP-ribose] polymerase 3
      Short name=PARP-3
    EC=2.4.2.30
Alternative name(s):
    ADPRT 3
    NAD(+) ADP-ribosyltransferase 3
    Poly[ADP-ribose] synthetase 3

Gene names

Name:

PARP3

Organism

Medicago truncatula (Barrel medic)

Taxonomic identifier

3880 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	799 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks By similarity.
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 1 SAP domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	DNA-binding NAD
Molecular function	Glycosyltransferase Transferase
PTM	ADP-ribosylation
Gene Ontology (GO)
Biological process	protein amino acid ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 799	799	Putative poly [ADP-ribose] polymerase 3		PRO_0000260501
Regions
	Domain	71 – 105	35	SAP
	Domain	165 – 261	97	BRCT
	Domain	436 – 555	120	PARP alpha-helical
	Domain	564 – 795	232	PARP catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q1SGF1-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 0B108316557FDE8A	FASTA	799	89,991
	10 20 30 40 50 60 MKVESRSHNV HHAHGEEEKV MTRKQKAESK AHEVEHSPKK AKVEDEKNGH TNGKSASDVV 70 80 90 100 110 120 QEYDEFCKAT NEQLSLEQMK EILEANDLDS SGSDLEITRR CQDLLFFGAL EKCMVCNGNM 130 140 150 160 170 180 EFDGRRYGCR GFYSEWSSCT FSTREPPRKD EPIKLPDSVQ NSPVSDLLKK YQDPSKRPQR 190 200 210 220 230 240 DLGLAIKPFT GMMISLMGRL NRTHLNFSGA SCLVASPAER DRGGTSKLAD AMERGIPVVR 250 260 270 280 290 300 EAWLTDSIEK QEPQPLESYD LVSDLSVDGK GIPWDKQDPG EEAIESLSAE LKLYGKRGVY 310 320 330 340 350 360 KDTKLHEQDG KIFEKDGILY NCAFSLCDQG RKLNDYCVMQ LIVVPENSLH LYFKKGRVGD 370 380 390 400 410 420 DPSAEERLEE CENVDNAIKE FVRLFEEITG NEFESWEREK KFQKKPLKFY PIDMDDGVEV 430 440 450 460 470 480 RHGALGLRQL GIAATHCKLE PMVANFMKVL CSQEIYKYAL MEMGYDSPDL PIGMVTNLHL 490 500 510 520 530 540 KRCEEILLEF IEKVKTLKET GPKADAIWSD FSQKWFTLMH STRPFIFRDY QEIADHAAAA 550 560 570 580 590 600 LEGVRDITLA SHLIGDMSGS TIDDPLSDTY KKLGCSITPL EKNSNDYEMI VKYLEKTYEP 610 620 630 640 650 660 VKVGDIEYGV SVENIFTVES SACPSYADIV KMPNKVLLWC GSRSSNLLRH LHKGFLPAIC 670 680 690 700 710 720 SLPVPGYMFG KAIVCSDAAA EAARYGFTAV DRPEGFLVLA IASLGNEITE LKSPPEDTTS 730 740 750 760 770 780 LEEKKVGVKG LGKKKTDESE HFVWKDDIKV PCGSIIASEH EDSPLEYNEY AVYDPKQVRI 790 SYLVGVKYEE KDAVIDTAE

References

[1]	The International Medicago Genome Annotation Group Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC144515 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.4.2.30. 266820.
Family and domain databases
InterPro	IPR001357. BRCT. IPR012982. PADR1. IPR012317. PARP_catalytic. IPR004102. PARP_reg. IPR008893. WGR. [Graphical view]
Gene3D	G3DSA:1.20.142.10. PARP_reg. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF08063. PADR1. 1 hit. PF00644. PARP. 1 hit. PF05406. WGR. 1 hit. [Graphical view]
SMART	SM00773. WGR. 1 hit. [Graphical view]
PROSITE	PS50172. BRCT. 1 hit. PS51060. PARP_ALPHA_HD. 1 hit. PS51059. PARP_CATALYTIC. 1 hit. PS50800. SAP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PARP3_MEDTR

Accession

Primary (citable) accession number: Q1SGF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)