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Protein

Growth factor receptor-bound protein 7

Gene

GRB7

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei239 – 2391Important for lipid binding and for stimulation of cell migrationBy similarity
Sitei511 – 5111Important for dimerization and for HRAS activationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Ligandi

Lipid-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-BTA-1306955. GRB7 events in ERBB2 signaling.
R-BTA-1433557. Signaling by SCF-KIT.
R-BTA-186763. Downstream signal transduction.
R-BTA-210993. Tie2 Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Growth factor receptor-bound protein 7
Alternative name(s):
Epidermal growth factor receptor GRB-7
GRB7 adapter protein
Gene namesi
Name:GRB7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

  • Cytoplasm By similarity
  • Cell projection By similarity
  • Cell junctionfocal adhesion By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cytoplasmic granule By similarity

  • Note: Predominantly cytoplasmic. Detected in stress granules where mRNA is stored under stress conditions.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 532532Growth factor receptor-bound protein 7PRO_0000245330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei188 – 1881Phosphotyrosine; by FAK1By similarity
Modified residuei361 – 3611PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on serine and threonine residues in response to activation of receptor kinases. Phosphorylated on tyrosine residues by TEK/TIE2. Phosphorylated on tyrosine residues by PTK2/FAK1, and possibly also other kinases. Phosphorylation is enhanced by activation of receptor kinases by a cognate ligand. Tyrosine phosphorylation is essential for activation of down-stream protein kinases. Phosphorylation decreases affinity for target mRNA molecules (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ1RMW5.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017366.

Interactioni

Subunit structurei

Homodimer. Interacts (via SH2 domain) with EGFR, ERBB2, ERBB3 (when phosphorylated), ERBB4 (when phosphorylated), EPHB1, INSR, FGFR1, PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Interacts with SHC1. Interacts with RND1. Interacts (when tyrosine phosphorylated) with FHL2 and HAX1 (By similarity). Interacts (via SH2 domain) with RET and PTK2/FAK1. Interacts (when not phosphorylated) with ELAVL1. In stressed cells, but not in normal cells, part of a complex that contains at least GRB7, PTK2/FAK1, STAU1, ELAVL1 and TIA1. Interacts (via SH2 domain) with KIT (phosphorylated) (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000023091.

Structurei

3D structure databases

ProteinModelPortaliQ1RMW5.
SMRiQ1RMW5. Positions 101-186, 369-405, 414-532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 18687Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini229 – 338110PHPROSITE-ProRule annotationAdd
BLAST
Domaini431 – 52797SH2PROSITE-ProRule annotationAdd
BLAST

Domaini

The PH domain mediates interaction with membranes containing phosphoinositides.By similarity

Sequence similaritiesi

Belongs to the GRB7/10/14 family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG3751. Eukaryota.
ENOG410XXC8. LUCA.
GeneTreeiENSGT00550000074537.
HOGENOMiHOG000231904.
HOVERGENiHBG000468.
InParanoidiQ1RMW5.
OMAiVQAAWPI.
OrthoDBiEOG091G135G.
TreeFamiTF317511.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR015042. BPS-dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR000980. SH2.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08947. BPS. 1 hit.
PF00169. PH. 1 hit.
PF00788. RA. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1RMW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELGLSPLHL SSSPEDLYLA SGTPPGTPPP LDAPLSGEVK RSQPLPIPTS
60 70 80 90 100
RKLLREEELQ STSLPSIPNP FPELCSPSSQ SPILGGSSSA RGLLPRDTSC
110 120 130 140 150
PHVIKVYSED GTCRSVEVAT GATARYVCEM LVQRSHALSD ENWGLVECHP
160 170 180 190 200
YLALERALED HESVAEVQAA WPIGGDSRIV FRKNFAKYEL FKSTPHSLFP
210 220 230 240 250
EKMVSSCLDA HTGMSHEDVI QNFLNAGSFP EIQGFLQLRG SGRKLWKRFF
260 270 280 290 300
CFLRRSGLYY STKGTSKDPR HLQYVADVNE SNVYVVTQGR KLYGMPTDFG
310 320 330 340 350
FCIKPNKLRN GHKGLRLFCT EDERSRSCWL AAFRLFKFGV QLYKNYQQTL
360 370 380 390 400
CRHMCPPCGG SPPSRSVSDD TLVAMDFSGH AGRVIENPRE ALSAALEEAQ
410 420 430 440 450
AWRKKTNHRL SLPTPSSGTS LSAAIHRTQP WFHGRISREE SQRLIRQQGL
460 470 480 490 500
VDGLFLVRES QRNPQGFVLS LCHVQKVKHY LILPSEEEGR LYFSMDDGLT
510 520 530
RFTDLLQLVE FHQLNRGILP CLLRYCCTRV AL
Length:532
Mass (Da):59,653
Last modified:May 16, 2006 - v1
Checksum:i8C8C23029FF717E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114669 mRNA. Translation: AAI14670.1.
RefSeqiNP_001039479.1. NM_001046014.1.
XP_005220751.1. XM_005220694.3.
XP_005220752.1. XM_005220695.3.
XP_005220753.1. XM_005220696.3.
XP_005220754.1. XM_005220697.2.
XP_015314313.1. XM_015458827.1.
UniGeneiBt.2949.

Genome annotation databases

EnsembliENSBTAT00000023091; ENSBTAP00000023091; ENSBTAG00000017366.
GeneIDi508847.
KEGGibta:508847.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114669 mRNA. Translation: AAI14670.1.
RefSeqiNP_001039479.1. NM_001046014.1.
XP_005220751.1. XM_005220694.3.
XP_005220752.1. XM_005220695.3.
XP_005220753.1. XM_005220696.3.
XP_005220754.1. XM_005220697.2.
XP_015314313.1. XM_015458827.1.
UniGeneiBt.2949.

3D structure databases

ProteinModelPortaliQ1RMW5.
SMRiQ1RMW5. Positions 101-186, 369-405, 414-532.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000023091.

Proteomic databases

PaxDbiQ1RMW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000023091; ENSBTAP00000023091; ENSBTAG00000017366.
GeneIDi508847.
KEGGibta:508847.

Organism-specific databases

CTDi2886.

Phylogenomic databases

eggNOGiKOG3751. Eukaryota.
ENOG410XXC8. LUCA.
GeneTreeiENSGT00550000074537.
HOGENOMiHOG000231904.
HOVERGENiHBG000468.
InParanoidiQ1RMW5.
OMAiVQAAWPI.
OrthoDBiEOG091G135G.
TreeFamiTF317511.

Enzyme and pathway databases

ReactomeiR-BTA-1306955. GRB7 events in ERBB2 signaling.
R-BTA-1433557. Signaling by SCF-KIT.
R-BTA-186763. Downstream signal transduction.
R-BTA-210993. Tie2 Signaling.

Gene expression databases

BgeeiENSBTAG00000017366.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR015042. BPS-dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR000980. SH2.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08947. BPS. 1 hit.
PF00169. PH. 1 hit.
PF00788. RA. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRB7_BOVIN
AccessioniPrimary (citable) accession number: Q1RMW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: May 16, 2006
Last modified: September 7, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.