Reviewed, UniProtKB/Swiss-Prot Q1RMS2 (PYRG2_BOVIN)

Names and origin

Protein names

Recommended name:
    CTP synthase 2
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase 2
    CTP synthetase 2

Gene names

Name:

CTPS2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	CTP synthase 2		PRO_0000247032
Regions
	Domain	300 – 554	255	Glutamine amidotransferase type-1
Sites
	Active site	399	1	For GATase activity By similarity
	Active site	526	1	For GATase activity By similarity
	Active site	528	1	For GATase activity By similarity
Amino acid modifications
	Modified residue	568	1	Phosphoserine By similarity
	Modified residue	571	1	Phosphoserine By similarity
	Modified residue	574	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RMS2-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 0A6E65BF35958831	FASTA	586	65,481
	10 20 30 40 50 60 MKYILVTGGV ISGIGKGIIA SSIGTILKSC GLRVTAIKID PYINIDAGTF SPYEHGEVFV 70 80 90 100 110 120 LNDGGEVDLD LGNYERFLDI NLYKDNNITT GKIYQHVINK ERRGDYLGKT VQVVPHITDA 130 140 150 160 170 180 VQEWVMNQAM VPVDGHKEEP QICVIELGGT IGDIEGMPFV EAFRQFQFKA KRENFCNIHV 190 200 210 220 230 240 SLVPQPSATG EQKTKPTQNS VRALRGLGLS PDLIVCRSST PIEMAVKEKI SMFCHVNPEQ 250 260 270 280 290 300 VICIHDVSST YRVPVLLEEQ GIIKYFKERL DLPIGDSASS LLSKWRNMAD RYERLQKTCS 310 320 330 340 350 360 IALVGKYTKL RDCYASVFKA LEHSALAINH KLNLMYIDSI DLEQTTEVED PVKFHEAWQK 370 380 390 400 410 420 LCKADGVLVP GGFGIRGTLG KLQAISWARS RKIPFLGVCL GMQLAVIEFA RNCLNLKDAD 430 440 450 460 470 480 STEFEPNARV PVVIDMPEHN PGNLGGTMRL GIRRTVFKTE NSILRKLYGD VPFIEERHRH 490 500 510 520 530 540 RYEVNPSLIS QLEQKDLSFV GQDVDGERME IIELANHPYF VGVQFHPEFS SRPMKPSPPY 550 560 570 580 LGLLLAATGN LNAYLLQGCK LSSSDRYSDA SDDSFSEPRL AELEIS

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT030529 mRNA. Translation: ABQ12969.1. BC114742 mRNA. Translation: AAI14743.1.
IPI	IPI00687870.
RefSeq	NP_001070434.1.
UniGene	Bt.74451
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000017017. Bos taurus. [Contig view]
GeneID	767849.
KEGG	bta:767849.
Phylogenomic databases
HOVERGEN	Q1RMS2.
OMA	Q1RMS2. KVPVDGN.
Enzyme and pathway databases
BRENDA	6.3.4.2. 251.
Family and domain databases
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG2_BOVIN

Accession

Primary (citable) accession number: Q1RMS2
Secondary accession number(s): A5D9B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)