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Q1RML2

- PLCZ1_BOVIN

UniProt

Q1RML2 - PLCZ1_BOVIN

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene

PLCZ1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (16 May 2006)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarity2 PublicationsCurated

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

    Cofactori

    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei170 – 1701By similarityPROSITE-ProRule annotation
    Active sitei215 – 2151By similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. calcium ion transport Source: Ensembl
    2. egg activation Source: Ensembl
    3. intracellular signal transduction Source: InterPro
    4. lipid catabolic process Source: UniProtKB-KW
    5. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Hydrolase, Transducer

    Keywords - Biological processi

    Fertilization, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_225102. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-zeta-1
    Phospholipase C-zeta-1By similarity
    Short name:
    PLC-zeta-1By similarity
    Gene namesi
    Name:PLCZ1By similarity
    Synonyms:PLCZImported
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    Nucleus By similarity. Cytoplasmperinuclear region By similarity
    Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: InterPro
    2. nucleus Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6346341-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347243Add
    BLAST

    Proteomic databases

    PRIDEiQ1RML2.

    Interactioni

    Subunit structurei

    Interacts (via its C2 domain) with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000017574.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1RML2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 7036EF-handPROSITE-ProRule annotationAdd
    BLAST
    Domaini155 – 299145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini376 – 492117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini497 – 598102C2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity.By similarity
    The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00730000110266.
    HOGENOMiHOG000006871.
    HOVERGENiHBG053610.
    InParanoidiQ1RML2.
    KOiK05861.
    OMAiTHADINY.
    OrthoDBiEOG7V49XT.
    TreeFamiTF313216.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028395. PLC-zeta1.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF29. PTHR10336:SF29. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1RML2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENKWFLLMV RDDFKGGKIT LEKALKLLEK LDIQCNTIHV KYIFKDNDRL    50
    KQGRITIEEF RTIYRIITYR EEIIEIFNTY SENRKILLEK NLVEFLMREQ 100
    YTLDFNKSIA SEIIQKYEPI EEVKQAHQMS FEGFRRYMDS SECLLFDNKC 150
    DHVYQDMTHP LTDYFISSSH NTYLISDQLW GPSDLWGYIS ALVKGCRCLE 200
    IDCWDGSQNE PVVYHGYTFT SKLLFKTVIQ AINKYAFLAS EYPVVLSLEN 250
    HCSPSQQEVM ADSLLATFGD ALLSYTLDNF SDRLPSPEAL KFKILVRNKK 300
    IGTLHETLER KGSDMHGKVE EFEEEEEIEQ EEDGSGAKEP EPVGDFQDDL 350
    AKEEQLKRVV GIPLFRKKKI KISMALSDLV IYTKVEKFKS FHHSHLYQQF 400
    NESNSIGESQ ARKLTKLAAR EFILHTRRFI TRVYPKALRA DSSNFNPQEF 450
    WNVGCQMVAL NFQTPGVPMD LQNGKFLDNG CSGYVLKPRF LRDKKTKFNP 500
    HKVQIDSNPL TLTIRLISGI QLPPSYQNKA DTLVIVEIFG VPNDQMKQQS 550
    RVIKKNAFNP RWNETFTFVI QVPELALIRF VAENQGLIAG NEFLGQYTLP 600
    VLCMNRGYRR VPLFSKMGES LEPASLFIYV WYIR 634
    Length:634
    Mass (Da):73,732
    Last modified:May 16, 2006 - v1
    Checksum:i84D721F03B8AE720
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti188 – 1881Y → H in AAV54518. 1 PublicationCurated
    Sequence conflicti436 – 4361K → Q in AAV54518. 1 PublicationCurated
    Sequence conflicti506 – 5061D → G in AAV54518. 1 PublicationCurated
    Sequence conflicti606 – 6061R → K in AAV54518. 1 PublicationCurated
    Sequence conflicti633 – 6331I → V in AAV54518. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY646356 mRNA. Translation: AAV54518.1.
    BC114836 mRNA. Translation: AAI14837.1.
    RefSeqiNP_001011680.2. NM_001011680.3.
    XP_005207032.1. XM_005206975.1.
    UniGeneiBt.37187.

    Genome annotation databases

    EnsembliENSBTAT00000017574; ENSBTAP00000017574; ENSBTAG00000013202.
    GeneIDi497026.
    KEGGibta:497026.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY646356 mRNA. Translation: AAV54518.1 .
    BC114836 mRNA. Translation: AAI14837.1 .
    RefSeqi NP_001011680.2. NM_001011680.3.
    XP_005207032.1. XM_005206975.1.
    UniGenei Bt.37187.

    3D structure databases

    ProteinModelPortali Q1RML2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000017574.

    Proteomic databases

    PRIDEi Q1RML2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000017574 ; ENSBTAP00000017574 ; ENSBTAG00000013202 .
    GeneIDi 497026.
    KEGGi bta:497026.

    Organism-specific databases

    CTDi 89869.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00730000110266.
    HOGENOMi HOG000006871.
    HOVERGENi HBG053610.
    InParanoidi Q1RML2.
    KOi K05861.
    OMAi THADINY.
    OrthoDBi EOG7V49XT.
    TreeFami TF313216.

    Enzyme and pathway databases

    Reactomei REACT_225102. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    NextBioi 20865839.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028395. PLC-zeta1.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF29. PTHR10336:SF29. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of PLC-zeta in cattle."
      Kumar K.G., Chomdej S., Wimmers K., Schellander K.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X AngusImported.
      Tissue: LiverImported.
    3. "Fertilization and inositol 1,4,5-trisphosphate (IP3)-induced calcium release in type-1 inositol 1,4,5-trisphosphate receptor down-regulated bovine eggs."
      Malcuit C., Knott J.G., He C., Wainwright T., Parys J.B., Robl J.M., Fissore R.A.
      Biol. Reprod. 73:2-13(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta."
      Ross P.J., Beyhan Z., Iager A.E., Yoon S.-Y., Malcuit C., Schellander K., Fissore R.A., Cibelli J.B.
      BMC Dev. Biol. 8:16-16(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPLCZ1_BOVIN
    AccessioniPrimary (citable) accession number: Q1RML2
    Secondary accession number(s): Q5IT24
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3