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Q1RML2

- PLCZ1_BOVIN

UniProt

Q1RML2 - PLCZ1_BOVIN

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene
PLCZ1, PLCZ
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarity2 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Cofactori

Calcium By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701 By similarityBy similarity
Active sitei215 – 2151 By similarityBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. calcium ion transport Source: Ensembl
  2. egg activation Source: Ensembl
  3. intracellular signal transduction Source: InterPro
  4. lipid catabolic process Source: UniProtKB-KW
  5. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Transducer

Keywords - Biological processi

Fertilization, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_225102. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-1
Short name:
PLC-zeta-1
Gene namesi
Name:PLCZ1
Synonyms:PLCZ
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 5

Subcellular locationi

Nucleus By similarity. Cytoplasmperinuclear region By similarity
Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity.By similarity

GO - Cellular componenti

  1. cytosol Source: InterPro
  2. nucleus Source: UniProtKB-SubCell
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6346341-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347243Add
BLAST

Proteomic databases

PRIDEiQ1RML2.

Interactioni

Subunit structurei

Interacts (via its C2 domain) with PtdIns3P and, to a lesser extent, PtdIns5P in vitro By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017574.

Structurei

3D structure databases

ProteinModelPortaliQ1RML2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 7036EF-handAdd
BLAST
Domaini155 – 299145PI-PLC X-boxAdd
BLAST
Domaini376 – 492117PI-PLC Y-boxAdd
BLAST
Domaini497 – 598102C2Add
BLAST

Domaini

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity By similarity.By similarity
The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization By similarity.By similarity

Sequence similaritiesi

Contains 1 C2 domain.
Contains 1 EF-hand domain.

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00730000110266.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ1RML2.
KOiK05861.
OMAiTHADINY.
OrthoDBiEOG7V49XT.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1RML2-1 [UniParc]FASTAAdd to Basket

« Hide

MENKWFLLMV RDDFKGGKIT LEKALKLLEK LDIQCNTIHV KYIFKDNDRL    50
KQGRITIEEF RTIYRIITYR EEIIEIFNTY SENRKILLEK NLVEFLMREQ 100
YTLDFNKSIA SEIIQKYEPI EEVKQAHQMS FEGFRRYMDS SECLLFDNKC 150
DHVYQDMTHP LTDYFISSSH NTYLISDQLW GPSDLWGYIS ALVKGCRCLE 200
IDCWDGSQNE PVVYHGYTFT SKLLFKTVIQ AINKYAFLAS EYPVVLSLEN 250
HCSPSQQEVM ADSLLATFGD ALLSYTLDNF SDRLPSPEAL KFKILVRNKK 300
IGTLHETLER KGSDMHGKVE EFEEEEEIEQ EEDGSGAKEP EPVGDFQDDL 350
AKEEQLKRVV GIPLFRKKKI KISMALSDLV IYTKVEKFKS FHHSHLYQQF 400
NESNSIGESQ ARKLTKLAAR EFILHTRRFI TRVYPKALRA DSSNFNPQEF 450
WNVGCQMVAL NFQTPGVPMD LQNGKFLDNG CSGYVLKPRF LRDKKTKFNP 500
HKVQIDSNPL TLTIRLISGI QLPPSYQNKA DTLVIVEIFG VPNDQMKQQS 550
RVIKKNAFNP RWNETFTFVI QVPELALIRF VAENQGLIAG NEFLGQYTLP 600
VLCMNRGYRR VPLFSKMGES LEPASLFIYV WYIR 634
Length:634
Mass (Da):73,732
Last modified:May 16, 2006 - v1
Checksum:i84D721F03B8AE720
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881Y → H in AAV54518. 1 Publication
Sequence conflicti436 – 4361K → Q in AAV54518. 1 Publication
Sequence conflicti506 – 5061D → G in AAV54518. 1 Publication
Sequence conflicti606 – 6061R → K in AAV54518. 1 Publication
Sequence conflicti633 – 6331I → V in AAV54518. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY646356 mRNA. Translation: AAV54518.1.
BC114836 mRNA. Translation: AAI14837.1.
RefSeqiNP_001011680.2. NM_001011680.3.
XP_005207032.1. XM_005206975.1.
UniGeneiBt.37187.

Genome annotation databases

EnsembliENSBTAT00000017574; ENSBTAP00000017574; ENSBTAG00000013202.
GeneIDi497026.
KEGGibta:497026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY646356 mRNA. Translation: AAV54518.1 .
BC114836 mRNA. Translation: AAI14837.1 .
RefSeqi NP_001011680.2. NM_001011680.3.
XP_005207032.1. XM_005206975.1.
UniGenei Bt.37187.

3D structure databases

ProteinModelPortali Q1RML2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000017574.

Proteomic databases

PRIDEi Q1RML2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000017574 ; ENSBTAP00000017574 ; ENSBTAG00000013202 .
GeneIDi 497026.
KEGGi bta:497026.

Organism-specific databases

CTDi 89869.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00730000110266.
HOGENOMi HOG000006871.
HOVERGENi HBG053610.
InParanoidi Q1RML2.
KOi K05861.
OMAi THADINY.
OrthoDBi EOG7V49XT.
TreeFami TF313216.

Enzyme and pathway databases

Reactomei REACT_225102. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi 20865839.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of PLC-zeta in cattle."
    Kumar K.G., Chomdej S., Wimmers K., Schellander K.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.
  3. "Fertilization and inositol 1,4,5-trisphosphate (IP3)-induced calcium release in type-1 inositol 1,4,5-trisphosphate receptor down-regulated bovine eggs."
    Malcuit C., Knott J.G., He C., Wainwright T., Parys J.B., Robl J.M., Fissore R.A.
    Biol. Reprod. 73:2-13(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta."
    Ross P.J., Beyhan Z., Iager A.E., Yoon S.-Y., Malcuit C., Schellander K., Fissore R.A., Cibelli J.B.
    BMC Dev. Biol. 8:16-16(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPLCZ1_BOVIN
AccessioniPrimary (citable) accession number: Q1RML2
Secondary accession number(s): Q5IT24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 16, 2006
Last modified: September 3, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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