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Q1RML2

- PLCZ1_BOVIN

UniProt

Q1RML2 - PLCZ1_BOVIN

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene

PLCZ1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarity2 PublicationsCurated

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Cofactori

Calcium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701By similarityPROSITE-ProRule annotation
Active sitei215 – 2151By similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. calcium ion transport Source: Ensembl
  2. egg activation Source: Ensembl
  3. intracellular signal transduction Source: InterPro
  4. lipid catabolic process Source: UniProtKB-KW
  5. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Transducer

Keywords - Biological processi

Fertilization, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_225102. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-1By similarity
Short name:
PLC-zeta-1By similarity
Gene namesi
Name:PLCZ1By similarity
Synonyms:PLCZImported
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 5

Subcellular locationi

Nucleus By similarity. Cytoplasmperinuclear region By similarity
Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis (By similarity).By similarity

GO - Cellular componenti

  1. cytosol Source: InterPro
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6346341-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347243Add
BLAST

Proteomic databases

PRIDEiQ1RML2.

Interactioni

Subunit structurei

Interacts (via its C2 domain) with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017574.

Structurei

3D structure databases

ProteinModelPortaliQ1RML2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 7036EF-handPROSITE-ProRule annotationAdd
BLAST
Domaini155 – 299145PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini376 – 492117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini497 – 598102C2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity.By similarity
The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ1RML2.
KOiK05861.
OMAiTHADINY.
OrthoDBiEOG7V49XT.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1RML2 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MENKWFLLMV RDDFKGGKIT LEKALKLLEK LDIQCNTIHV KYIFKDNDRL
60 70 80 90 100
KQGRITIEEF RTIYRIITYR EEIIEIFNTY SENRKILLEK NLVEFLMREQ
110 120 130 140 150
YTLDFNKSIA SEIIQKYEPI EEVKQAHQMS FEGFRRYMDS SECLLFDNKC
160 170 180 190 200
DHVYQDMTHP LTDYFISSSH NTYLISDQLW GPSDLWGYIS ALVKGCRCLE
210 220 230 240 250
IDCWDGSQNE PVVYHGYTFT SKLLFKTVIQ AINKYAFLAS EYPVVLSLEN
260 270 280 290 300
HCSPSQQEVM ADSLLATFGD ALLSYTLDNF SDRLPSPEAL KFKILVRNKK
310 320 330 340 350
IGTLHETLER KGSDMHGKVE EFEEEEEIEQ EEDGSGAKEP EPVGDFQDDL
360 370 380 390 400
AKEEQLKRVV GIPLFRKKKI KISMALSDLV IYTKVEKFKS FHHSHLYQQF
410 420 430 440 450
NESNSIGESQ ARKLTKLAAR EFILHTRRFI TRVYPKALRA DSSNFNPQEF
460 470 480 490 500
WNVGCQMVAL NFQTPGVPMD LQNGKFLDNG CSGYVLKPRF LRDKKTKFNP
510 520 530 540 550
HKVQIDSNPL TLTIRLISGI QLPPSYQNKA DTLVIVEIFG VPNDQMKQQS
560 570 580 590 600
RVIKKNAFNP RWNETFTFVI QVPELALIRF VAENQGLIAG NEFLGQYTLP
610 620 630
VLCMNRGYRR VPLFSKMGES LEPASLFIYV WYIR
Length:634
Mass (Da):73,732
Last modified:May 16, 2006 - v1
Checksum:i84D721F03B8AE720
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881Y → H in AAV54518. 1 PublicationCurated
Sequence conflicti436 – 4361K → Q in AAV54518. 1 PublicationCurated
Sequence conflicti506 – 5061D → G in AAV54518. 1 PublicationCurated
Sequence conflicti606 – 6061R → K in AAV54518. 1 PublicationCurated
Sequence conflicti633 – 6331I → V in AAV54518. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY646356 mRNA. Translation: AAV54518.1.
BC114836 mRNA. Translation: AAI14837.1.
RefSeqiNP_001011680.2. NM_001011680.3.
XP_005207032.1. XM_005206975.1.
UniGeneiBt.37187.

Genome annotation databases

EnsembliENSBTAT00000017574; ENSBTAP00000017574; ENSBTAG00000013202.
GeneIDi497026.
KEGGibta:497026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY646356 mRNA. Translation: AAV54518.1 .
BC114836 mRNA. Translation: AAI14837.1 .
RefSeqi NP_001011680.2. NM_001011680.3.
XP_005207032.1. XM_005206975.1.
UniGenei Bt.37187.

3D structure databases

ProteinModelPortali Q1RML2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000017574.

Proteomic databases

PRIDEi Q1RML2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000017574 ; ENSBTAP00000017574 ; ENSBTAG00000013202 .
GeneIDi 497026.
KEGGi bta:497026.

Organism-specific databases

CTDi 89869.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00760000118936.
HOGENOMi HOG000006871.
HOVERGENi HBG053610.
InParanoidi Q1RML2.
KOi K05861.
OMAi THADINY.
OrthoDBi EOG7V49XT.
TreeFami TF313216.

Enzyme and pathway databases

Reactomei REACT_225102. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi 20865839.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of PLC-zeta in cattle."
    Kumar K.G., Chomdej S., Wimmers K., Schellander K.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X AngusImported.
    Tissue: LiverImported.
  3. "Fertilization and inositol 1,4,5-trisphosphate (IP3)-induced calcium release in type-1 inositol 1,4,5-trisphosphate receptor down-regulated bovine eggs."
    Malcuit C., Knott J.G., He C., Wainwright T., Parys J.B., Robl J.M., Fissore R.A.
    Biol. Reprod. 73:2-13(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta."
    Ross P.J., Beyhan Z., Iager A.E., Yoon S.-Y., Malcuit C., Schellander K., Fissore R.A., Cibelli J.B.
    BMC Dev. Biol. 8:16-16(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPLCZ1_BOVIN
AccessioniPrimary (citable) accession number: Q1RML2
Secondary accession number(s): Q5IT24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 16, 2006
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3