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Q1RML1

- UBE2S_BOVIN

UniProt

Q1RML1 - UBE2S_BOVIN

Protein

Ubiquitin-conjugating enzyme E2 S

Gene

UBE2S

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. activation of anaphase-promoting complex activity Source: UniProtKB
    2. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
    3. exit from mitosis Source: UniProtKB
    4. free ubiquitin chain polymerization Source: UniProtKB
    5. protein K11-linked ubiquitination Source: UniProtKB
    6. protein K27-linked ubiquitination Source: UniProtKB
    7. protein K29-linked ubiquitination Source: UniProtKB
    8. protein K63-linked ubiquitination Source: UniProtKB
    9. protein K6-linked ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 S (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein S
    Ubiquitin-protein ligase S
    Gene namesi
    Name:UBE2S
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 18

    Subcellular locationi

    GO - Cellular componenti

    1. anaphase-promoting complex Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 223223Ubiquitin-conjugating enzyme E2 SPRO_0000245039Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Post-translational modificationi

    Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.By similarity

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Proteomic databases

    PRIDEiQ1RML1.

    Interactioni

    Subunit structurei

    Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ1RML1.
    SMRiQ1RML1. Positions 6-156.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110565.
    HOGENOMiHOG000233455.
    HOVERGENiHBG063308.
    InParanoidiQ1RML1.
    KOiK10583.
    OMAiPDLGIKH.
    OrthoDBiEOG73JKXD.
    TreeFamiTF101120.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1RML1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSNVENLPP HIIRLVYKEV TTLTADPPDG IKVFPNEEDL TDLQVTIEGP    50
    EGTPYAGGLF RMKLLLGKDF PASPPKGYFL TKIFHPNVGA NGEICVNVLK 100
    RDWTAELGIR HVLLTIKCLL IHPNPESALN EEAGRLLLEN YEEYAARARL 150
    LTEIHGGAGG PSGGRPEPGR ATASGAAAST ADPTAPGGPA GAEGPMAKKH 200
    AGERDKKLAA KKKTDKKRAL RRL 223
    Length:223
    Mass (Da):23,896
    Last modified:June 28, 2011 - v2
    Checksum:iC3D0FAEE6429E3EB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti186 – 1861P → T in AAI14839. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC114838 mRNA. Translation: AAI14839.1.
    BC148138 mRNA. Translation: AAI48139.1.
    RefSeqiNP_001069940.1. NM_001076472.2.
    UniGeneiBt.21408.

    Genome annotation databases

    EnsembliENSBTAT00000012136; ENSBTAP00000012136; ENSBTAG00000009211.
    GeneIDi617703.
    KEGGibta:617703.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC114838 mRNA. Translation: AAI14839.1 .
    BC148138 mRNA. Translation: AAI48139.1 .
    RefSeqi NP_001069940.1. NM_001076472.2.
    UniGenei Bt.21408.

    3D structure databases

    ProteinModelPortali Q1RML1.
    SMRi Q1RML1. Positions 6-156.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q1RML1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000012136 ; ENSBTAP00000012136 ; ENSBTAG00000009211 .
    GeneIDi 617703.
    KEGGi bta:617703.

    Organism-specific databases

    CTDi 27338.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110565.
    HOGENOMi HOG000233455.
    HOVERGENi HBG063308.
    InParanoidi Q1RML1.
    KOi K10583.
    OMAi PDLGIKH.
    OrthoDBi EOG73JKXD.
    TreeFami TF101120.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 20900803.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus and Hereford.
      Tissue: Fetal brain and Liver.

    Entry informationi

    Entry nameiUBE2S_BOVIN
    AccessioniPrimary (citable) accession number: Q1RML1
    Secondary accession number(s): A6QLZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3