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Q1RML1

- UBE2S_BOVIN

UniProt

Q1RML1 - UBE2S_BOVIN

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Protein

Ubiquitin-conjugating enzyme E2 S

Gene

UBE2S

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. activation of anaphase-promoting complex activity Source: UniProtKB
  2. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  3. exit from mitosis Source: UniProtKB
  4. free ubiquitin chain polymerization Source: UniProtKB
  5. protein K11-linked ubiquitination Source: UniProtKB
  6. protein K27-linked ubiquitination Source: UniProtKB
  7. protein K29-linked ubiquitination Source: UniProtKB
  8. protein K63-linked ubiquitination Source: UniProtKB
  9. protein K6-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 S (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein S
Ubiquitin-protein ligase S
Gene namesi
Name:UBE2S
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 18

Subcellular locationi

GO - Cellular componenti

  1. anaphase-promoting complex Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Ubiquitin-conjugating enzyme E2 SPRO_0000245039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Post-translational modificationi

Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

PRIDEiQ1RML1.

Interactioni

Subunit structurei

Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL By similarity.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ1RML1.
SMRiQ1RML1. Positions 6-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ1RML1.
KOiK10583.
OMAiPDLGIKH.
OrthoDBiEOG73JKXD.
TreeFamiTF101120.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1RML1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSNVENLPP HIIRLVYKEV TTLTADPPDG IKVFPNEEDL TDLQVTIEGP
60 70 80 90 100
EGTPYAGGLF RMKLLLGKDF PASPPKGYFL TKIFHPNVGA NGEICVNVLK
110 120 130 140 150
RDWTAELGIR HVLLTIKCLL IHPNPESALN EEAGRLLLEN YEEYAARARL
160 170 180 190 200
LTEIHGGAGG PSGGRPEPGR ATASGAAAST ADPTAPGGPA GAEGPMAKKH
210 220
AGERDKKLAA KKKTDKKRAL RRL
Length:223
Mass (Da):23,896
Last modified:June 28, 2011 - v2
Checksum:iC3D0FAEE6429E3EB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861P → T in AAI14839. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC114838 mRNA. Translation: AAI14839.1.
BC148138 mRNA. Translation: AAI48139.1.
RefSeqiNP_001069940.1. NM_001076472.2.
UniGeneiBt.21408.

Genome annotation databases

EnsembliENSBTAT00000012136; ENSBTAP00000012136; ENSBTAG00000009211.
GeneIDi617703.
KEGGibta:617703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC114838 mRNA. Translation: AAI14839.1 .
BC148138 mRNA. Translation: AAI48139.1 .
RefSeqi NP_001069940.1. NM_001076472.2.
UniGenei Bt.21408.

3D structure databases

ProteinModelPortali Q1RML1.
SMRi Q1RML1. Positions 6-156.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q1RML1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000012136 ; ENSBTAP00000012136 ; ENSBTAG00000009211 .
GeneIDi 617703.
KEGGi bta:617703.

Organism-specific databases

CTDi 27338.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00730000110565.
HOGENOMi HOG000233455.
HOVERGENi HBG063308.
InParanoidi Q1RML1.
KOi K10583.
OMAi PDLGIKH.
OrthoDBi EOG73JKXD.
TreeFami TF101120.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

NextBioi 20900803.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus and Hereford.
    Tissue: Fetal brain and Liver.

Entry informationi

Entry nameiUBE2S_BOVIN
AccessioniPrimary (citable) accession number: Q1RML1
Secondary accession number(s): A6QLZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 28, 2011
Last modified: October 1, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3