Reviewed, UniProtKB/Swiss-Prot Q1RLY6 (KMO_DANRE)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase

Gene names

Name:	kmo
ORF Names:	zgc:136684

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion outer membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	NAD metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	kynurenine 3-monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Kynurenine 3-monooxygenase		PRO_0000361909
Regions
	Transmembrane	393 – 412	20	Potential
	Transmembrane	433 – 453	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RLY6-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 4BBC8BA0F664BA61	FASTA	474	54,247
	10 20 30 40 50 60 METAFSHPPQ SSSSKRKVIA VVGGGLVGSL NACFLAKRGF DVEVYESRED IRQAKVVKGR 70 80 90 100 110 120 SINLALSHRG RQALKHVGME DKIISKGIPM HARMIHNVNG KRSPIPYGKK GQYILSVDRA 130 140 150 160 170 180 NLNKELLTAA EAYPNTRLNF NHKLHDWSPK TGTMTFIGSD GQKTETQADL IVGCDGAFSA 190 200 210 220 230 240 VRKQFLRQSR FNYSQTYIPH GYMELTMPPK DGDFAMEPNY LHIWPRNTFM MIALPNLDRT 250 260 270 280 290 300 FTCTLFMPFE DFEKIRTGDE LLRFFHKYFP DSVPLIGVEA LKQDFFRLPA QAMVSVKCCP 310 320 330 340 350 360 YHLFEKCVLM GDAAHAVVPF YGQGMNAGFE DCLVFDEIMD QFNENLVAVL QEYTRVRVPD 370 380 390 400 410 420 DHAIADLAMY NYIEMRAHVN SKYFIFRKYL DNLLHFFMPK TIVPLYTMVT FTRTRYNDAV 430 440 450 460 470 NRWHWQNKVI TRGLWLCGFV SAAGGTYVLV KNSHKLPSIS AEQLWTRILA LKLF

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC115227 mRNA. Translation: AAI15228.1.
IPI	IPI00921737.
UniGene	Dr.79892
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RLY6.
Proteomic databases
PRIDE	Q1RLY6.
Genome annotation databases
Ensembl	ENSDART00000003706; ENSDARP00000024051; ENSDARG00000009160; Danio rerio. [Genome view]
Organism-specific databases
ZFIN	ZDB-GENE-030424-4. kmo.
Phylogenomic databases
HOGENOM	Q1RLY6.
HOVERGEN	Q1RLY6.
Gene expression databases
ArrayExpress	Q1RLY6.
Bgee	Q1RLY6.
Family and domain databases
InterPro	IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_DANRE

Accession

Primary (citable) accession number: Q1RLY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	May 16, 2006
Last modified:	October 13, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project