Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4

Gene

B3gnt4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4 (EC:2.4.1.149By similarity)
Alternative name(s):
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4 (EC:2.4.1.-)
Short name:
BGnT-4
Short name:
Beta-1,3-Gn-T4
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 4
Short name:
Beta3Gn-T4
Gene namesi
Name:B3gnt4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2680208. B3gnt4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini26 – 350LumenalSequence analysisAdd BLAST325

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892081 – 350N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4Add BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi53N-linked (GlcNAc...)Sequence analysis1
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ1RLK6.
PRIDEiQ1RLK6.

PTM databases

PhosphoSitePlusiQ1RLK6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029431.
GenevisibleiQ1RLK6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031384.

Structurei

3D structure databases

ProteinModelPortaliQ1RLK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ1RLK6.
KOiK07971.
OMAiHHAGFKT.
OrthoDBiEOG091G0AXM.
TreeFamiTF318639.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1RLK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRLGCVLF CSLVVLLLSC LLLLKERIPA GSSKAHQQFL ALPRSHHSQC
60 70 80 90 100
SPNLTVVNTS LSLPSRHRLF LTYRHCRNFS ILLEPSECAR DTFLLLVIKS
110 120 130 140 150
QPAHIEQRSA IRSTWGRAGS WARGRQLKLV FLLGVAGPVP PAQLLVYESW
160 170 180 190 200
QFDDILQWDF AEDFFNLTLK ELHVQRWIAA ACTQAHFILK GDDDVFIHVP
210 220 230 240 250
NVLEFLEGWD PAQDFLVGDV IRLARPNRNT KVKYFIPFSM YRARHYPPYA
260 270 280 290 300
GGGGYVMSQA TVRHLHMAME EAELFPIDDV FVGMCLRKLG VTPIHHAGFK
310 320 330 340 350
TFGIQQPLNP RDPCLYKGLL LVHRLSPLEM WTMWALVTDE RLKCAATHKP
Length:350
Mass (Da):39,873
Last modified:July 27, 2011 - v2
Checksum:i3086DAE555E1C336
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3P → S in AAK68856 (Ref. 1) Curated1
Sequence conflicti3P → S in AAI14988 (PubMed:15489334).Curated1
Sequence conflicti3P → S in AAI15756 (PubMed:15489334).Curated1
Sequence conflicti267M → R in AAK68856 (Ref. 1) Curated1
Sequence conflicti267M → T in AAI14988 (PubMed:15489334).Curated1
Sequence conflicti267M → T in AAI15756 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037786 mRNA. Translation: AAK68856.1.
AC157931 Genomic DNA. No translation available.
BC114987 mRNA. Translation: AAI14988.1.
BC115755 mRNA. Translation: AAI15756.1.
CCDSiCCDS19665.1.
RefSeqiNP_941013.2. NM_198611.2.
XP_006530374.1. XM_006530311.3.
XP_006530376.1. XM_006530313.3.
XP_011246502.1. XM_011248200.2.
UniGeneiMm.484068.

Genome annotation databases

EnsembliENSMUST00000031384; ENSMUSP00000031384; ENSMUSG00000029431.
GeneIDi231727.
KEGGimmu:231727.
UCSCiuc008znw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037786 mRNA. Translation: AAK68856.1.
AC157931 Genomic DNA. No translation available.
BC114987 mRNA. Translation: AAI14988.1.
BC115755 mRNA. Translation: AAI15756.1.
CCDSiCCDS19665.1.
RefSeqiNP_941013.2. NM_198611.2.
XP_006530374.1. XM_006530311.3.
XP_006530376.1. XM_006530313.3.
XP_011246502.1. XM_011248200.2.
UniGeneiMm.484068.

3D structure databases

ProteinModelPortaliQ1RLK6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031384.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSitePlusiQ1RLK6.

Proteomic databases

PaxDbiQ1RLK6.
PRIDEiQ1RLK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031384; ENSMUSP00000031384; ENSMUSG00000029431.
GeneIDi231727.
KEGGimmu:231727.
UCSCiuc008znw.1. mouse.

Organism-specific databases

CTDi79369.
MGIiMGI:2680208. B3gnt4.

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ1RLK6.
KOiK07971.
OMAiHHAGFKT.
OrthoDBiEOG091G0AXM.
TreeFamiTF318639.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-913709. O-linked glycosylation of mucins.

Miscellaneous databases

PROiQ1RLK6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029431.
GenevisibleiQ1RLK6. MM.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB3GN4_MOUSE
AccessioniPrimary (citable) accession number: Q1RLK6
Secondary accession number(s): E9QPF4, Q923H4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.