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Q1RKJ6 (MNME_RICBR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA modification GTPase MnmE
EC=3.6.-.-
Gene names
Name:mnmE
Synonyms:trmE
Ordered Locus Names:RBE_0037
OrganismRickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]
Taxonomic identifier336407 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP MF_00379

Cofactor

Binds 1 potassium ion per subunit By similarity. HAMAP MF_00379

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP MF_00379

Subcellular location

Cytoplasm By similarity HAMAP MF_00379.

Sequence similarities

Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily.

Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA modification

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445tRNA modification GTPase MnmE HAMAP MF_00379
PRO_0000277958

Regions

Domain227 – 330104G
Nucleotide binding225 – 2306GTP By similarity
Nucleotide binding244 – 2507GTP By similarity
Nucleotide binding269 – 2724GTP By similarity

Sites

Metal binding2251Potassium By similarity
Metal binding2291Magnesium By similarity
Metal binding2441Potassium; via carbonyl oxygen By similarity
Metal binding2461Potassium; via carbonyl oxygen By similarity
Metal binding2491Potassium By similarity
Metal binding2501Magnesium By similarity
Binding site201Formyltetrahydrofolate By similarity
Binding site791Formyltetrahydrofolate By similarity
Binding site1191Formyltetrahydrofolate By similarity
Binding site4451Formyltetrahydrofolate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1RKJ6 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: C123942CE67227FF

FASTA44549,570
        10         20         30         40         50         60 
METIFAQSSS FGKAGVAVFR VSGTKSLEVL KLLTGKSNFK PRFMYYQKLT SPESNDLIDN 

        70         80         90        100        110        120 
AMVVYFKAPN SFTGEDVVEI HTHGSKAISI MLTNALLNIP GVRLAEAGEF TKRAFLNNKF 

       130        140        150        160        170        180 
DLTAAEGIAD LINAETIMQH KQAIRQAGGA LEELYNSWRS QLLRIISLLE AYIDFPDEDI 

       190        200        210        220        230        240 
PESVLKDVTN THKTLINTIS EYLNDNRKGE LLRSGLKLAI IGPPNAGKSS LLNFLMRRDI 

       250        260        270        280        290        300 
AIVSNIAGTT RDIIEGHLDI GGYPIILQDT AGIRGESNDV IEQEGIKRAI DSAKKADIKI 

       310        320        330        340        350        360 
VMFDAETLDS AVNEDITGLI DENTIVIINK IDLIPENRIF NIENKYRCLK VSIKNNIALP 

       370        380        390        400        410        420 
SILKNIEEIA ENMAGFTETP YITNERHRHH LKQALVYLKD FNLDNDLVLA TEDIRMTMRR 

       430        440 
IGAITGIIDV EEILGEIFKN FCIGK

« Hide

References

[1]"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RML369-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000087 Genomic DNA. Translation: ABE04118.1.
RefSeqYP_537207.1. NC_007940.1.

3D structure databases

ProteinModelPortalQ1RKJ6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ1RKJ6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3996084.
GenomeReviewsGene locus RBE_0037 in contig CP000087_GR.
KEGGrbe:RBE_0037.
PATRIC17881313. VBIRicBel102610_0041.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0486.
HOGENOMHBG746795.
OMALIDNAMV.
ProtClustDBPRK05291.

Enzyme and pathway databases

BioCycRBEL336407:RBE_0037-MONOMER.

Family and domain databases

HAMAPMF_00379. GTPase_MnmE.
[Tree]
InterProIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_TrmE.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
Gene3DG3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.
KOK03650.
PfamPF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00450. MnmE_trmE_thdF. 1 hit.
TIGR00231. Small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNME_RICBR
AccessionPrimary (citable) accession number: Q1RKJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 16, 2006
Last modified: January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia bellii strain RML369-C

Rickettsia bellii (strain RML369-C): entries and gene names

SIMILARITY comments

Index of protein domains and families

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