Q1RKI1 (LIPB_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Octanoyltransferase
EC=2.3.1.181

Alternative name(s):
Lipoate-protein ligase B
Lipoyl/octanoyl transferase
Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Gene names

Name:	lipB
Ordered Locus Names:	RBE_0052

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate By similarity. HAMAP MF_00013
Catalytic activity	Octanoyl-[acyl-carrier-protein] + protein = protein N(6)-(octanoyl)lysine + [acyl-carrier-protein]. HAMAP MF_00013
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2. HAMAP MF_00013
Subcellular location	Cytoplasm Potential HAMAP MF_00013.
Miscellaneous	In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes By similarity. HAMAP MF_00013
Sequence similarities	Belongs to the LipB family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipoate biosynthetic process Inferred from electronic annotation. Source: InterPro protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	lipoyl(octanoyl) transferase activity Inferred from electronic annotation. Source: EC octanoyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Octanoyltransferase HAMAP MF_00013		PRO_0000242759
Sites
	Active site	174	1	Acyl-thioester intermediate By similarity
	Site	140	1	Lowers pKa of active site Cys By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RKI1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: FAD670FC862236D0	FASTA	209	23,828
	10 20 30 40 50 60 MVQFITIPTP IDYQDSLMLM EDYVNKVIDG EAPEAVYLVE HLDVYTAGTN YKQEELLNHN 70 80 90 100 110 120 NIPVIYTGRG GKFTFHGAGQ RVIYPILNLA LPNRTKDLKL YVRMLEEWII NSLDIFGIKA 130 140 150 160 170 180 YIIKNKVGIW VKTNDDIPSK IAAIGVRVRK WVTYHGIAIN ISTDLSKFNG IIPCGLENSL 190 200 VTSLNQLGVY IEMTEFDKIL EVEFIKIFK

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE04133.1.
RefSeq	YP_537222.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RKI1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3995016.
GenomeReviews	Gene locus RBE_0052 in contig CP000087_GR.
KEGG	rbe:RBE_0052.
PATRIC	17881347. VBIRicBel102610_0058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0321.
HOGENOM	HBG716160.
OMA	HAMQRFT.
ProtClustDB	PRK14347.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0052-MONOMER.
Family and domain databases
HAMAP	MF_00013. LipB. [Tree]
InterPro	IPR004143. BPL_LipA_LipB. IPR000544. Octanoyltransferase. IPR020605. Octanoyltransferase_CS. [Graphical view]
KO	K03801.
PANTHER	PTHR10993:SF0. PTHR10993:SF0. 1 hit.
Pfam	PF03099. BPL_LplA_LipB. 1 hit. [Graphical view]
PIRSF	PIRSF016262. LPLase. 1 hit.
TIGRFAMs	TIGR00214. LipB. 1 hit.
PROSITE	PS01313. LIPB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPB_RICBR

Accession

Primary (citable) accession number: Q1RKI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program