Q1RKI0 (GREA_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription elongation factor GreA

Alternative name(s):
Transcript cleavage factor GreA

Gene names

Name:	greA
Ordered Locus Names:	RBE_0053

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group › Rickettsia bellii

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides By similarity. HAMAP-Rule MF_00105

Sequence similarities

Belongs to the GreA/GreB family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Domain	Coiled coil
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	regulation of DNA-dependent transcription, elongation Inferred from electronic annotation. Source: HAMAP transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	Transcription elongation factor GreA HAMAP-Rule MF_00105		PRO_0000277900
Regions
	Coiled coil	44 – 69	26	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RKI0 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 2D90AA76DC96BC8A	FASTA	162	18,115
	10 20 30 40 50 60 MMAKFPITDQ GFEKLEHELK HLKHVERKKV SEDIAEARAH GDLSENAEYE AAREKQAFVE 70 80 90 100 110 120 ARIKHLEDIT ARAEIINVAK LSGDSIKFGA TVVLIDDETE EEVMYHIVGE YEADITKKRV 130 140 150 160 SIASPIAKAL IGKSVGDIVE VMTPGGVKSY EVVTIKYEEL VF

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE04134.1.
RefSeq	YP_537223.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RKI0.
ModBase	Search...
Protein-protein interaction databases
STRING	336407.RBE_0053.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE04134; ABE04134; RBE_0053.
GeneID	3995017.
KEGG	rbe:RBE_0053.
PATRIC	17881349. VBIRicBel102610_0059.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0782.
HOGENOM	HOG000241145.
KO	K03624.
OMA	HNEGRIA.
ProtClustDB	PRK00226.
Enzyme and pathway databases
BioCyc	RBEL336407:GJCY-55-MONOMER.
Family and domain databases
Gene3D	1.10.287.180. 1 hit. 3.10.50.30. 1 hit.
HAMAP	MF_00105. GreA_GreB.
InterPro	IPR018151. TF_GreA/GreB_CS. IPR006359. Tscrpt_elong_fac_GreA. IPR023459. Tscrpt_elong_fac_GreA/B. IPR001437. Tscrpt_elong_fac_GreA/B_C. IPR022691. Tscrpt_elong_fac_GreA/B_N. [Graphical view]
Pfam	PF01272. GreA_GreB. 1 hit. PF03449. GreA_GreB_N. 1 hit. [Graphical view]
PIRSF	PIRSF006092. GreA_GreB. 1 hit.
SUPFAM	SSF46557. GreA_GreB. 1 hit.
TIGRFAMs	TIGR01462. greA. 1 hit.
PROSITE	PS00829. GREAB_1. 1 hit. PS00830. GREAB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GREA_RICBR

Accession

Primary (citable) accession number: Q1RKI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program