Reviewed, UniProtKB/Swiss-Prot Q1RKI0 (GREA_RICBR)

Names and origin

Protein names

Recommended name:
    Transcription elongation factor greA
Alternative name(s):
    Transcript cleavage factor greA

Gene names

Name:	greA
Ordered Locus Names:	RBE_0053

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as greA or greB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides By similarity.

Sequence similarities

Belongs to the greA/greB family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Domain	Coiled coil
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP transcription elongation regulator activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	Transcription elongation factor greA HAMAP MF_00105		PRO_0000277900
Regions
	Coiled coil	44 – 69	26	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RKI0-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 2D90AA76DC96BC8A	FASTA	162	18,115
	10 20 30 40 50 60 MMAKFPITDQ GFEKLEHELK HLKHVERKKV SEDIAEARAH GDLSENAEYE AAREKQAFVE 70 80 90 100 110 120 ARIKHLEDIT ARAEIINVAK LSGDSIKFGA TVVLIDDETE EEVMYHIVGE YEADITKKRV 130 140 150 160 SIASPIAKAL IGKSVGDIVE VMTPGGVKSY EVVTIKYEEL VF

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04134.1.
RefSeq	YP_537223.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKI0.
Genome annotation databases
GeneID	3995017.
GenomeReviews	Gene locus RBE_0053 in contig CP000087_GR.
KEGG	rbe:RBE_0053.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RKI0.
OMA	IEGRIQE
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0053-MON.
Family and domain databases
HAMAP	MF_00105. [Tree]
InterPro	IPR006359. GreA. IPR001437. GreA_GreB_reg. IPR018151. TF_GreA/GreB_CS. [Graphical view]
Gene3D	G3DSA:1.10.287.180. GreA_GreB_reg. 1 hit.
Pfam	PF01272. GreA_GreB. 1 hit. PF03449. GreA_GreB_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01462. greA. 1 hit.
PROSITE	PS00829. GREAB_1. 1 hit. PS00830. GREAB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GREA_RICBR

Accession

Primary (citable) accession number: Q1RKI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 16, 2006
Last modified:	November 24, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)