Q1RKH8 (PRIM_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase
EC=2.7.7.-

Gene names

Name:	dnaG
Ordered Locus Names:	RBE_0055

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis.
Cofactor	Binds 1 zinc ion per monomer By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA primase family. Contains 1 Toprim domain.

Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA primase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	DNA primase		PRO_0000280941
Regions
	Domain	250 – 332	83	Toprim
	Zinc finger	38 – 62	25	CHC2-type By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RKH8 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 78CFA5F1AA2B4780	FASTA	599	68,875
	10 20 30 40 50 60 MKLAPEFYEV LRNRINISDV VRQKVVLTRK SGNYVGLCPF HQEKTPSFTV SDSKRFFYCF 70 80 90 100 110 120 GCKAAGDVIK FTSNISGLSY NDSAIKLATD YGVEIPKLTQ KQKEFYEESD EILNILELAN 130 140 150 160 170 180 KFFKSQLTPE ILNYLNERNI TNETIKEFSI GFAPRGNKFE KFFHDKNITN VQLGKAGLIG 190 200 210 220 230 240 KREDGEIYSL FSNRITIPIR NIYNKIVGFG GRVIGQGLPK YLNSPETVVF QKSETLYGEH 250 260 270 280 290 300 KAISSSYKKN YSILVEGYFD VISLHQAGFS EVVASLGTSV TENHLHKLWR AGDEIILCLD 310 320 330 340 350 360 GDSAGIKASV RTINLALPLI NSEKKISFIR LPTGLDPDDA VNKNGVDFFT KLIDTRISLS 370 380 390 400 410 420 EMIWQIEYAG KNFKTAEDKA NLEKNLKDYC SKISDSNLRA SYYRFFKDQI WQNLVTKQQK 430 440 450 460 470 480 TVAKNVNLLP LSSGYSELEI LEHAFCALLI KFPQILQEFE IKEFILNLNF SNKLLEEFRN 490 500 510 520 530 540 WYLSEVIDNA VEAGEIAAIV EKTSFFDIFL LLSEADNLFL DIAFNKDNVR LDLLWQWLYK 550 560 570 580 590 RYYLLNLQQE YAHITKEYVI TNIDDYEKVL FYKKEILKIA SELQDLNESF INHIIDNSK

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE04136.1.
RefSeq	YP_537225.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RKH8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKH8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3995019.
GenomeReviews	Gene locus RBE_0055 in contig CP000087_GR.
KEGG	rbe:RBE_0055.
PATRIC	17881353. VBIRicBel102610_0061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0358.
HOGENOM	HBG586351.
OMA	MLEHAFC.
ProtClustDB	PRK05667.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0055-MONOMER.
Family and domain databases
InterPro	IPR013264. DNA_primase_core_N. IPR006295. DNA_primase_DnaG. IPR006171. Toprim_domain. IPR002694. Znf_CHC2. [Graphical view]
Gene3D	G3DSA:3.90.980.10. Toprim_N. 1 hit. G3DSA:3.90.580.10. Znf_CHC2. 1 hit.
KO	K02316.
Pfam	PF01751. Toprim. 1 hit. PF08275. Toprim_N. 1 hit. PF01807. zf-CHC2. 1 hit. [Graphical view]
SMART	SM00493. TOPRIM. 1 hit. SM00400. ZnF_CHCC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01391. DnaG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRIM_RICBR

Accession

Primary (citable) accession number: Q1RKH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program