Q1RKH3 (TRUA_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Ordered Locus Names:	RBE_0060

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP MF_00171
Subunit structure	Homodimer By similarity. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_0000278055
Sites
	Active site	52	1	Nucleophile By similarity
	Binding site	111	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RKH3 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: BDECC4BB92801A49	FASTA	245	27,982
	10 20 30 40 50 60 MYRYKISVEY LGTNLAGWQR QSGVMSVQQI LEEAIYKFSG EQVTLFGSGR TDAGVHAIGQ 70 80 90 100 110 120 IAHFDLSKYF EPYKIIRAIN YFTRPYIVGV CNCELVDNNF HARFSAIARH YVYRILNRPY 130 140 150 160 170 180 PSVIDFDRVW WISVPLDVSA MQKSSLYLLG KHNFTSFRAS SCQSKSPIKT LTKLDIIKEN 190 200 210 220 230 240 EEIKLYFSAP SFLHHMVRNI VGSLVLVGKN IWQAEQMQQV LEVADRKAAG PTAPACGLYF IKADY

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE04141.1.
RefSeq	YP_537230.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RKH3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3995024.
GenomeReviews	Gene locus RBE_0060 in contig CP000087_GR.
KEGG	rbe:RBE_0060.
PATRIC	17881363. VBIRicBel102610_0066.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HBG621020.
OMA	RHYRYRI.
ProtClustDB	PRK00021.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0060-MONOMER.
Family and domain databases
HAMAP	MF_00171. TruA. [Tree]
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
KO	K06173.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. HisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_RICBR

Accession

Primary (citable) accession number: Q1RKH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program