Reviewed, UniProtKB/Swiss-Prot Q1RKG2 (SYA_RICBR)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	RBE_0071

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	877 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm HAMAP MF_00036.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 877	877	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000278068

Sequences

	Sequence		Length	Mass (Da)
	Q1RKG2-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 983BD61A275CF38F	FASTA	877	98,911
	10 20 30 40 50 60 MSKFTTEEIR SKFISYFKAN NHTHVSSSPL IPHNDPSLMF VNSGMVQFKN VFTGQEKRPY 70 80 90 100 110 120 DKAVTSQKSL RAGGKHNDLE NVGYTARHHT FFEMLGNFSF GDYFKEQAIY YAWNLLTKEF 130 140 150 160 170 180 ELPKDKLYAT VYHTDDEAAS YWKKIAGFSD DRIIRIKTND NFWSMGDLGP CGPCSEIFYD 190 200 210 220 230 240 HGEQIYGGLP GTKDEDGDRF IEIWNMVFMQ YEQINKDTRI DLPKKSIDTG MGLERMTAVL 250 260 270 280 290 300 QHVNNNYDID LFQEIISFTE NILKVKVEAE AKFSYRVIAD HLRACSFLIA DGVVPSSEGR 310 320 330 340 350 360 GYVLRRIMRR AMRHAHILGS KEPLMYKLLP KLVDLMGNTY PELKRAESFI SSILEQEEIR 370 380 390 400 410 420 FKTTLERGLK LLSEETENLN AGGRLSGEVA FKLYDTYGFP LDLTEDILKN RNIAVDHQGF 430 440 450 460 470 480 EQQMLEQKEC ARKSWLGSGE SKTDQLWFDI KAKYGSTEFL GYNLNEADGK IVALIKDNAL 490 500 510 520 530 540 VNDISEIDTQ FLLIANQTPF YGESGGQMGD IGVIKTQNCE IEVIDTLKYL GFIIVHKCVL 550 560 570 580 590 600 KKGKVITDEN ANFSIDVKYR QNLRIHHSAT HLLHAVLHQI LGKHVTQKGS LVATSYLRFD 610 620 630 640 650 660 ISHPKALTPE EIVLIEDKVN EIIRDNHEVD TTLMSTEDAV KQGAMALFGE KYDSEVRVVK 670 680 690 700 710 720 MGETSLELCG GTHVRHTGDI GCFKITSESA IAAGIRRIEA VCGEFVIKLI REKEGLLKNI 730 740 750 760 770 780 ENSLKTNKNE LVSKVNNILE RNKELEKELE KTYLASLDLS VEQIEKQAEK IKEIKLIYCH 790 800 810 820 830 840 VENLDNKILR QAAENLTKKV EDLVVVYVGN NSSKLSITVG ISRAITDKFN AGFIAKELSL 850 860 870 FLGGSGGGGQ PSIAQAGGSD LAKLPKVKDQ LQSLLDA

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04152.1.
RefSeq	YP_537241.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKG2.
Genome annotation databases
GeneID	3994975.
GenomeReviews	Gene locus RBE_0071 in contig CP000087_GR.
KEGG	rbe:RBE_0071.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	GHTIEPS.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0071-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_RICBR

Accession

Primary (citable) accession number: Q1RKG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 16, 2006
Last modified:	December 15, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)