Reviewed, UniProtKB/Swiss-Prot Q1RKF9 (DPO3A_RICBR)

Names and origin

Protein names

Recommended name:
    DNA polymerase III subunit alpha
    EC=2.7.7.7

Gene names

Name:	dnaE
Ordered Locus Names:	RBE_0074

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	1172 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1172	1172	DNA polymerase III subunit alpha		PRO_0000280955

Sequences

	Sequence		Length	Mass (Da)
	Q1RKF9-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 2A1B7972C2E6AE9B	FASTA	1,172	131,797
	10 20 30 40 50 60 MQTEFIHLRT QSSYSFLASA LTTEKIVELA SSYKMAAICL TDKENLFGSL EFALYAIKKG 70 80 90 100 110 120 LQPIHGVILN IQYETGVFAE ILLIAKDETG YKNLLKLSSI TFTTNDRKIC NHITFEDLKE 130 140 150 160 170 180 HQEGLITLCC YTEGVIGKCL LANKEEQAEM FARNLQEIFG DRFYFEIMRH DLPEEQLIED 190 200 210 220 230 240 NYIKIASKLN IPLVATNKVL FSKKSMHDAH DVLLCISAGV TKEYPDRKTV SENCYFKSAK 250 260 270 280 290 300 EMIELFADLP SAIENTVNLT QRCYFAAHTN PPMLPNFATK DISETDLIRK EAKDGLLARL 310 320 330 340 350 360 DTKFKSEHIS IEHQESIKTE YFARLDYELN IICSMNFAGY FLIVSDFIKW SKKQGILVGP 370 380 390 400 410 420 GRGSGAGSVV AWSLLITDLD PIKFGLLFER FLNPDRISMP DFDIDFCQER REEVINYVRS 430 440 450 460 470 480 KYGNNRVGQI ITFGKMQAKA VIKDVARVLS LPYKFADYLT ELVPFSAINP VTLEQAIREV 490 500 510 520 530 540 PELANAAKGN GLYNLEGELE LIKLVLDTSL ILEGLHRHSS THAAGIVIAG TDLVDIVPVY 550 560 570 580 590 600 KDANSDMLVV GYSMKYCELA GLIKFDFLGL QTLTVITDCK KLLKEQNIEI DFNDMTFDDE 610 620 630 640 650 660 KTYQMLCKGK GVGVFQFESV GMKDALRRLK PDSIHDLIAL GALYRPGPME NIPTYIACKH 670 680 690 700 710 720 KLQQPDYLHE LLKPILEETY GVVIYQEQVQ RIAQVLAGYT LGAADLLRRA MGKKIKAEME 730 740 750 760 770 780 QQEEIFVKGA IANNISEAQA KSIFATVAKF AGYGFNKAHA AAYGVISYQT AYLKANYPAE 790 800 810 820 830 840 FVVACLNLEL NNHDKINLFL QEAKDNNIKI IAPNINISGG YFSVKSVIPR LDCGIHGDQL 850 860 870 880 890 900 KDTAVKPQYD IDSSIIFALG AIKGVTPNFG KLVTDERNAR GAFKSIVDFI ERLPPKAINS 910 920 930 940 950 960 KLLENLIKAG CFDELHDNRL QLFLSISKLL AYSVSYHEEQ ASNQFSLIKV SSLSKEILVS 970 980 990 1000 1010 1020 SDYADKNTLA FYEFEAMGLF ISNHPLTEYK EIFNRLNILS SADLHNNLPD GTNRVMIAGV 1030 1040 1050 1060 1070 1080 IQKKDSRMSA RGRFVTLVLS DPENIFELSI FSEEVLKDYV HLLDVKSLVV VNCDIIKDEG 1090 1100 1110 1120 1130 1140 GIKITAKSFS SIENATGNQQ FDLQLYPKND EELEQIITLL SARINNDEQS NTIATIYLSN 1150 1160 1170 KSVKNFVAKI TFPEKFFLKG QDFEILSIYQ NT

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04155.1.
RefSeq	YP_537244.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKF9.
Genome annotation databases
GeneID	3994978.
GenomeReviews	Gene locus RBE_0074 in contig CP000087_GR.
KEGG	rbe:RBE_0074.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RKF9.
OMA	QIVTFGT
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0074-MON.
Family and domain databases
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3A_RICBR

Accession

Primary (citable) accession number: Q1RKF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	May 16, 2006
Last modified:	November 24, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)