Reviewed, UniProtKB/Swiss-Prot Q1RKE6 (NUOL_RICBR)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit L
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit L
    NDH-1 subunit L

Gene names

Name:	nuoL
Ordered Locus Names:	RBE_0087

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 5 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	NADH-quinone oxidoreductase subunit L		PRO_0000287835
Regions
	Transmembrane	4 – 24	21	Potential
	Transmembrane	31 – 51	21	Potential
	Transmembrane	88 – 108	21	Potential
	Transmembrane	114 – 134	21	Potential
	Transmembrane	137 – 157	21	Potential
	Transmembrane	171 – 191	21	Potential
	Transmembrane	213 – 233	21	Potential
	Transmembrane	255 – 275	21	Potential
	Transmembrane	287 – 307	21	Potential
	Transmembrane	315 – 335	21	Potential
	Transmembrane	340 – 360	21	Potential
	Transmembrane	383 – 403	21	Potential
	Transmembrane	418 – 438	21	Potential
	Transmembrane	467 – 487	21	Potential
	Transmembrane	513 – 533	21	Potential
	Transmembrane	622 – 642	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RKE6-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: EEB9B220C02E6A66	FASTA	642	71,911
	10 20 30 40 50 60 MYKSIAIMII LLPLASALIN GLFVRRIDKK LASIVATSFL SLSALFALII FYHTGLNGHI 70 80 90 100 110 120 IHIKLLPWIE VSQFKVDWSI YIDQLTSIMF IAVTWVSSVV HIYSLGYMSR DKGIVRFLSF 130 140 150 160 170 180 LSLFTFFMLM LVSADNFLQL FFGWEGVGIC SYLLIGFWYS KESANKAAIK AFIANRVGDF 190 200 210 220 230 240 AFILGVITII FYCHSANYED VFLLAPKLAN TKILLADFEI SILDIACLLL FIGCMGKSAQ 250 260 270 280 290 300 IGLHVWLPDA MEGPTPVSAL IHAATMVTAG VFLVARCSYL FEYSPMVLQF ITIIGGITCL 310 320 330 340 350 360 FAASIAIMQN DIKKIIAYST CSQLGYMFMA CGVSAYNSGI FHLVTHAFFK ALLFLSAGSV 370 380 390 400 410 420 IHAVHEQDIF KMGELRNKMP ITYGNFLIGS LALIGIYPLA GFYSKDSILE AAYSSGSFMF 430 440 450 460 470 480 IFGILAAILT AIYSMKIIML VFHGKTRLEK DVFEHAHEPP KVMNNPLTLL VAGSFFSGMI 490 500 510 520 530 540 GYYLLSMDKP NGYFHDSLLN LHAYKLLITH PPLHIKLLPM VVGIIGIVVG IYLYKSDVVM 550 560 570 580 590 600 SFLRRQESSK DNYFTKILIN KYYFDELYNF LIVKPINCLA CLFYSGDQKI IDRFGPNGFA 610 620 630 640 RSVNCFSRLT GKTQTGYVFN YTLYVVLFVV VTISYFVWLV AV

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04168.1.
RefSeq	YP_537257.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKE6.
Genome annotation databases
GeneID	3995078.
GenomeReviews	Gene locus RBE_0087 in contig CP000087_GR.
KEGG	rbe:RBE_0087.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RKE6.
OMA	KLLPMAV
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0087-MON.
Family and domain databases
InterPro	IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003916. NADH_UbQ_OxRdtase_chain5. IPR001516. NADH_UbQ_OxRdtase_chain5/L_N. IPR003945. NADHpl_OxRdtase_5. IPR018393. NADHpl_OxRdtase_5_sg. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. PF00662. Oxidored_q1_N. 1 hit. [Graphical view]
PRINTS	PR01434. NADHDHGNASE5. PR01435. NPOXDRDTASE5.
TIGRFAMs	TIGR01974. NDH_I_L. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NUOL_RICBR

Accession

Primary (citable) accession number: Q1RKE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 16, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)