Reviewed, UniProtKB/Swiss-Prot Q1RKE5 (NUOM_RICBR)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit M
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit M
    NDH-1 subunit M

Gene names

Name:	nuoM
Ordered Locus Names:	RBE_0088

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 4 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	NADH-quinone oxidoreductase subunit M		PRO_0000272334
Regions
	Transmembrane	5 – 25	21	Potential
	Transmembrane	37 – 57	21	Potential
	Transmembrane	89 – 109	21	Potential
	Transmembrane	115 – 135	21	Potential
	Transmembrane	139 – 159	21	Potential
	Transmembrane	172 – 192	21	Potential
	Transmembrane	216 – 236	21	Potential
	Transmembrane	251 – 271	21	Potential
	Transmembrane	280 – 300	21	Potential
	Transmembrane	308 – 328	21	Potential
	Transmembrane	334 – 354	21	Potential
	Transmembrane	375 – 395	21	Potential
	Transmembrane	411 – 431	21	Potential
	Transmembrane	458 – 478	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RKE5-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 0D4F88FBC2542E6E	FASTA	494	55,539
	10 20 30 40 50 60 MLELPIISIT IFLPLISVLY ILLFISQSKK PDKQIHTMYV AVLSSVLTFI STIYILIEFD 70 80 90 100 110 120 SSNPTYQFVE RYSWLDKIGL EFHVGIDGIS IFFVSLTSFL TLICIIGSLF TVKKYIKEYL 130 140 150 160 170 180 VCFLLMESFC IGAFTSINLL VFYLFFEVIL VPMYIIIGVW GGENRIYAAV KFFLYTFFGS 190 200 210 220 230 240 VFFLLSIIYI YSKIHSFDLS NIGEFTNNFP LHVQQILWWA IFIAFAIKIP MIPFHTWLPD 250 260 270 280 290 300 AHVQAPTSGS VILAGILLKL GGYGFLRVLL PLLPNASQEF AIYVIWLSVI AIIYASLVAL 310 320 330 340 350 360 AQKDMKKMIA YSSITHMGYV TIGIFSFTDS GVSGALFQML SHGIISSCLF LIVGTLYERL 370 380 390 400 410 420 HTKEIAKYGG VASKMPVLAT FFMIAMLGSV GLPGTSGFIG EFLSLLGIYK VNVIATFLAA 430 440 450 460 470 480 LGIILGAIYM LKLYKEVMLG EITNKEIINF RDLYIYEIIS IAPLILLIIY FGLMPSSILN 490 VFHLSVENLL VKFF

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04169.1.
RefSeq	YP_537258.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKE5.
Genome annotation databases
GeneID	3995079.
GenomeReviews	Gene locus RBE_0088 in contig CP000087_GR.
KEGG	rbe:RBE_0088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG643593.
OMA	ANWIERF.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0088-MON.
Family and domain databases
InterPro	IPR010227. NADH_Q_OxRdtase_chainM. IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003918. NADH_UbQ_OxRdtase_chain4. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
PRINTS	PR01437. NUOXDRDTASE4.
ProtoNet	Search...

Entry information

Entry name

NUOM_RICBR

Accession

Primary (citable) accession number: Q1RKE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	May 16, 2006
Last modified:	December 15, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)