Reviewed, UniProtKB/Swiss-Prot Q1RKD5 (ACON_RICBR)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:	acnA
Ordered Locus Names:	RBE_0098

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 885	885	Aconitate hydratase		PRO_0000272342
Sites
	Metal binding	425	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	491	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	494	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RKD5-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 11EBBC6C35DCD29E	FASTA	885	97,508
	10 20 30 40 50 60 MQKMHNSEYL KELSVNNISY KIYDINKAAS DIDLPLKKLP YSLRVLFENV LRTGSKQNLM 70 80 90 100 110 120 VFKEWLKNKK SDAEIDFMPA RVLMQDFTGV PAIVDLAAMR DAMKKIGGDP LKINPLIPVD 130 140 150 160 170 180 LVIDHSVSVD SYASGSSFDK NVAMEMRRNI ERYQFLKWGQ QAFNNFKVVP PGTGICHQVN 190 200 210 220 230 240 LEYLAKVVWH SNGVAYPDSL VGTDSHTTMV NGLSVLGWGV GGIEAEAAML GQPLTMILPE 250 260 270 280 290 300 VIGVKLTGKL TGTATATDLV LKITEMLRKK KVVGKFVEFY GEGLKAMTIA DRATISNMAP 310 320 330 340 350 360 EYGATCGFFP IDQETIKYLE LTGRDKEQIK LVEEYAKAQD LWCNFDDAAE YTDILELDLS 370 380 390 400 410 420 EVTSSLAGPR RPQDRVNLGD VSSGFKKELS TFSSNNISID TKHAVANQNY EIGNGDVVIA 430 440 450 460 470 480 AITSCTNTSN PSVMIGAALL AKKAIEQGLK VKPWVKTSLA PGSKVVTEYL KSSGLNQYLD 490 500 510 520 530 540 QLGFNLVGYG CTTCIGNSGP LNPEIEETIN KNGLVVASVL SGNRNFEGRI NPLTKASYLA 550 560 570 580 590 600 SPILVVAYAL SGSLNIDLTN HPLGKNDKGR DVYLKDIWPS KEEIDKVIAN SINSSMFVEK 610 620 630 640 650 660 YSDIFSGTKE WQSLEVTSSS NYAWDKSSTY INNPPYFENI GSKNSIKDIK SARILAIFGD 670 680 690 700 710 720 SITTDHISPA GSISKTSPAA KYLTDHQISP IDFNSYGSRR GNHEVMMRGT FANIRIKNEM 730 740 750 760 770 780 CKGVEGGFTI NQLKNMQQTI YDAAMDYKAN GVSAVIFAGK EYGSGSSRDW AAKGPQLLGV 790 800 810 820 830 840 KAVIAESFER IHRSNLVGMG VLPLIFTNNM TRFDLKLDGS ESIDIIGLNE HIKPYNSVKC 850 860 870 880 IIKKQNGEMQ TIDLILQIFT DNEINYIKHG SIMHFVVENL KNNHI

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE04179.1.
RefSeq	YP_537268.1.
3D structure databases
SMR	Q1RKD5. Positions 8-880.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKD5.
Genome annotation databases
GeneID	3995632.
GenomeReviews	Gene locus RBE_0098 in contig CP000087_GR.
KEGG	rbe:RBE_0098.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1048.
HOGENOM	HBG289738.
OMA	YSKAQGM.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0098-MONOMER.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_RICBR

Accession

Primary (citable) accession number: Q1RKD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	May 16, 2006
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)