Reviewed, UniProtKB/Swiss-Prot Q1RKD1 (NUOH_RICBR)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit H
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit H
    NDH-1 subunit H

Gene names

Name:	nuoH
Ordered Locus Names:	RBE_0102

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01350
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	NADH-quinone oxidoreductase subunit H HAMAP MF_01350		PRO_0000244948
Regions
	Transmembrane	9 – 29	21	Potential
	Transmembrane	50 – 70	21	Potential
	Transmembrane	82 – 102	21	Potential
	Transmembrane	115 – 135	21	Potential
	Transmembrane	161 – 181	21	Potential
	Transmembrane	187 – 207	21	Potential
	Transmembrane	235 – 255	21	Potential
	Transmembrane	275 – 295	21	Potential
	Transmembrane	311 – 331	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RKD1-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 83FC5DAB714E2686	FASTA	339	38,111
	10 20 30 40 50 60 MIELFFEYIF PLIIIALKVV AITIPLILCV AYLTYAERRV IGLMQLRRGP NVVGPFGLLQ 70 80 90 100 110 120 PIADAVKLLF KEPIIPTNAD KILFVLAPMI TFILSLIGWA VIPFAKGVVL ADINVGVLYI 130 140 150 160 170 180 LAISSLSVYG IIIAGWASNS KYAFLGAIRS SAQMISYEVS MGLVIITVLL TTGTLNLSQI 190 200 210 220 230 240 VEAQRTMPWW IDLMLMPMGV VFFISVLAET NRLPFDLPEA ESELVAGYNV EYSSMGFALF 250 260 270 280 290 300 FLGEYANMIL VSAMTTTFFL GGYLPPFNIS WLDCIPGFFW FVFKVGFLLF CFLWIRATLP 310 320 330 RYRYDQLMRL GWKVFLPLTL FWVVLVSSVL IYTDHLPNV

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04183.1.
RefSeq	YP_537272.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKD1.
Genome annotation databases
GeneID	3995636.
GenomeReviews	Gene locus RBE_0102 in contig CP000087_GR.
KEGG	rbe:RBE_0102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RKD1.
OMA	GIIMGGW
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0102-MON.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOH_RICBR

Accession

Primary (citable) accession number: Q1RKD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	May 16, 2006
Last modified:	November 24, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)