Reviewed, UniProtKB/Swiss-Prot Q1RKC5 (PBPA_RICBR)

Names and origin

Protein names

Recommended name:
    Penicillin-binding protein 1A
      Short name=PBP-1a
      Short name=PBP1a
Including the following 2 domains:
    1- Recommended name:
            Penicillin-insensitive transglycosylase
              EC=2.4.2.-
        Alternative name(s):
            Peptidoglycan TGase
    2- Recommended name:
            Penicillin-sensitive transpeptidase
              EC=3.4.-.-
        Alternative name(s):
            DD-transpeptidase

Gene names

Name:	mrcA
Synonyms:	ponA
Ordered Locus Names:	RBE_0108

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	791 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	In the N-terminal section; belongs to the glycosyltransferase 51 family. In the C-terminal section; belongs to the transpeptidase family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell peptidoglycan-based cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	penicillin binding Inferred from electronic annotation. Source: InterPro peptidase activity Inferred from electronic annotation. Source: InterPro transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 791	791	Penicillin-binding protein 1A		PRO_0000286450
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 791	764	Periplasmic Potential
	Region	49 – 220	172	Transglycosylase
	Region	398 – 711	314	Transpeptidase
Sites
	Active site	457	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RKC5-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 7D013204EC44294F	FASTA	791	88,946
	10 20 30 40 50 60 MYKSLFFCLK ILALLFLIGC GIVAYIIYYY SRDLPDYRQL ARYYPPSVTR IYSRDGKLME 70 80 90 100 110 120 EYAFERRVFI PINSIPTSLK ESFIAAEDKN FYNHQGVDLF GIVRAAFLNI SNFLHHRRME 130 140 150 160 170 180 GASTITQQVV KNFLLTNEVS FQRKIKEAIL SYMISRVFTK DQILELYLNQ TFFGRGAYGV 190 200 210 220 230 240 ATAAQNYFNK SVEELTIAES AFIAALPKAP SELNPEKNYA RVKARRDYVI MRMLEDGYIT 250 260 270 280 290 300 SDAAKEAVDS PITLRKRDQD ETVTADYYAE QVRDEVIKML GSKDEFYTSG LTIITSLDAK 310 320 330 340 350 360 MQKFAEESLR KGLRDFDRKR GFRKAIATIS LDNWQEELKK LPTTPSLLEY KIAVVLEVTD 370 380 390 400 410 420 TQAEIGFASG AKSKIPISEM KWAKSELKSA KKLLTKGDVI VVEPVKDYYA LRQIPEVNGA 430 440 450 460 470 480 IMVMNPNTGQ VLTSVGGYDF FASKFDRVTQ ALRQPGSLSK TFVYLAALEN GVKPNQIFND 490 500 510 520 530 540 GPIEISQGPG MPSWRPKNYE GKFLGPITMR TGLEKSRNLV TVRVATAVGL TKIVDIIKRF 550 560 570 580 590 600 GINDNPQKVY SMVLGSIETT LERITNAYGI IANGGKRIKP HFVELIKDRN GKVIYRRDDR 610 620 630 640 650 660 ECTTCNVSDN DLDNAILEIP AENIYMVTDA ASDYQITSLL TGVVDRGTAY SAKKLGKVIG 670 680 690 700 710 720 GKTGTSNDSK DTWFIGFTPK IVVGSYVGYD TPRTLGRRAT GSSVVLPIFI DFMSNAYKDE 730 740 750 760 770 780 PPLPFKVPDS VKLQAVDRAT GQITPSGSVM EAFKINNILI IEDDSEIIDN NHNNDVFDYV 790 PTEKDQSQEI Y

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04189.1.
RefSeq	YP_537278.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKC5.
Protein family/group databases
CAZy	GT51. Glycosyltransferase Family 51.
Genome annotation databases
GeneID	3994983.
GenomeReviews	Gene locus RBE_0108 in contig CP000087_GR.
KEGG	rbe:RBE_0108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RKC5.
OMA	NIQMLEN
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0108-MON.
Family and domain databases
InterPro	IPR012338. B-lactamase-typ_transpept_fold. IPR001264. Glyco_trans_51. IPR011816. PBP_1a. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF00912. Transgly. 1 hit. PF00905. Transpeptidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR02074. PBP_1a_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PBPA_RICBR

Accession

Primary (citable) accession number: Q1RKC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 16, 2006
Last modified:	November 24, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)