Q1RKC2 (MIAB_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
EC=2.-.-.-

Alternative name(s):
tRNA-i(6)A37 methylthiotransferase

Gene names

Name:	miaB
Ordered Locus Names:	RBE_0111

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP MF_01864
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Monomer By similarity. HAMAP MF_01864
Subcellular location	Cytoplasm Potential HAMAP MF_01864.
Sequence similarities	Belongs to the methylthiotransferase family. MiaB subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP MF_01864		PRO_0000374503
Regions
	Domain	3 – 124	122	MTTase N-terminal
	Domain	383 – 446	64	TRAM
Sites
	Metal binding	12	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	87	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	162	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	166	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	169	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RKC2 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: F4BBFEC933BCF7C6	FASTA	446	50,714
	10 20 30 40 50 60 MSKKLYIKTY GCQMNVYDSV KMQDLLYPYG YEPTENIEEA DVIILNTCHI REKAAEKTYS 70 80 90 100 110 120 ELGRIKKLQD ARKKQGLNSA IIVVAGCVAQ AEGEEIFTRT PYVDIVVGPQ SYYNLPELIS 130 140 150 160 170 180 KVVRHEKHLI DLDFVEEAKF DQLPEQLYPQ GASSFISVQE GCDKFCTFCV VPYTRGAEFS 190 200 210 220 230 240 RNVEQVYREA LKIVSSGAKE IMLLGQNVNA YHGKTSDDKV FTLADLIRHL VKIPNLERLR 250 260 270 280 290 300 YTTSHPIDMT DDLISLHGLE PKLMPFLHLP VQSGSNKTLK AMNRKHDRDY YFDIIDRLRK 310 320 330 340 350 360 ARADIVLSSD FIVGFPGETD EDFADTLDLV RKVKYGQCYS FKYSPRPGTP GATRTDQVPE 370 380 390 400 410 420 HIKSERLTIL QKELAAQQLA FNESCIGSTM KVLFDRDGKF DDQIIGKTPY MQSVYIKNPN 430 440 KDLLGKIIEV KITKAALNSL SGEIYR

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE04192.1.
RefSeq	YP_537281.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RKC2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RKC2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3994986.
GenomeReviews	Gene locus RBE_0111 in contig CP000087_GR.
KEGG	rbe:RBE_0111.
PATRIC	17881486. VBIRicBel102610_0127.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0621.
HOGENOM	HBG457663.
OMA	EEDKFDH.
ProtClustDB	PRK14325.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0111-MONOMER.
Family and domain databases
HAMAP	MF_01864. tRNA_metthiotr_MiaB. [Tree]
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/B12-bd/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR006463. MiaB_methiolase. IPR007197. rSAM. IPR023404. rSAM_horseshoe. IPR002792. TRAM. [Graphical view]
Gene3D	G3DSA:3.80.30.20. G3DSA:3.80.30.20. 1 hit.
KO	K06168.
PANTHER	PTHR11918. UPF0004. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF01938. TRAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01574. MiaB-methiolase. 1 hit. TIGR00089. TIGR00089. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIAB_RICBR

Accession

Primary (citable) accession number: Q1RKC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program