Reviewed, UniProtKB/Swiss-Prot Q1RK48 (LPXK_RICBR)

Names and origin

Protein names

Recommended name:
    Tetraacyldisaccharide 4'-kinase
    EC=2.7.1.130
Alternative name(s):
    Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	RBE_0185

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity.
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the lpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_0000277955
Regions
	Nucleotide binding	54 – 61	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RK48-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 1225B4076699AB75	FASTA	321	36,014
	10 20 30 40 50 60 MIKLLYPKFW QKRNIIAYIL FPISWIYQFL GFLRHVFARP VILPAKVICV GNCSVGGTGK 70 80 90 100 110 120 TQIVMYLANL LKTQNIDFVI VTKAYGSNLK EATIVKAEHS TLEVGDESVI LAKHGTVIAT 130 140 150 160 170 180 KNIKQILPLI HELKPSIIIV DDFLQNPYFH KDITIVSVDR QRLFGNGFLI PAGPLREYPN 190 200 210 220 230 240 RALSAADLVF LVGNNSGGIP VSLASYANKL IQAQIVASSD IDKNKNYFAF SGIGNPERFF 250 260 270 280 290 300 LTLKNYGLNI VGYKIFPDHY NYLQEDLENL SLLAKTNNAT LITTRKDYVK IGDSSDIIVC 310 320 LDVKLSINNP NLLYEKIFKK D

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04266.1.
RefSeq	YP_537355.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RK48.
Genome annotation databases
GeneID	3995329.
GenomeReviews	Gene locus RBE_0185 in contig CP000087_GR.
KEGG	rbe:RBE_0185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RK48.
OMA	VILMDDG
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0185-MON.
Family and domain databases
HAMAP	MF_00409. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. lpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_RICBR

Accession

Primary (citable) accession number: Q1RK48

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 16, 2006
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)