Reviewed, UniProtKB/Swiss-Prot Q1RK29 (KSGA_RICBR)

Names and origin

Protein names

Recommended name:
    Dimethyladenosine transferase
    EC=2.1.1.-
Alternative name(s):
    S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
    16S rRNA dimethylase
    High level kasugamycin resistance protein ksgA
    Kasugamycin dimethyltransferase

Gene names

Name:	ksgA
Ordered Locus Names:	RBE_0204

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. Its inactivation leads to kasugamycin resistance By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance rRNA processing
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: HAMAP response to antibiotic Inferred from electronic annotation. Source: HAMAP
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Dimethyladenosine transferase HAMAP MF_00607		PRO_0000257338
Sites
	Binding site	23	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	25	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	50	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	72	1	S-adenosyl-L-methionine By similarity
	Binding site	97	1	S-adenosyl-L-methionine By similarity
	Binding site	116	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RK29-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 3122E85751E72C9D	FASTA	268	29,656
	10 20 30 40 50 60 MLPSIAKHAA SHQIHPLKKH GQNFIFDGSL CDKIVRASGL EENSNVLEIG PGTGGLTRSI 70 80 90 100 110 120 LHKNPKLLTV IETDERCIPL LNEIKQYHPN LNIIKQDALK LKLSDLNTNK ITIISNLPYH 130 140 150 160 170 180 IGTELVIRWL KESSLVASMT LMLQKEVVER ICAKPSTKAY GRLSVICSLI ATVEKCFDVA 190 200 210 220 230 240 PTAFYPPPKV YSAIVKLTPL ENIPNSDLIS KVELITKMAF AGRRKMIKSS LKNLAPNISE 250 260 LLAKLNISDN CRAENLTPND YLSLASLI

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04285.1.
RefSeq	YP_537374.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RK29.
Genome annotation databases
GeneID	3995968.
GenomeReviews	Gene locus RBE_0204 in contig CP000087_GR.
KEGG	rbe:RBE_0204.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RK29.
OMA	NELMENK
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0204-MON.
Family and domain databases
HAMAP	MF_00607. [Tree]
InterPro	IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR001737. rRNA_Ade_methylase_transferase. IPR020598. rRNA_Ade_methylase_Trfase_N. IPR011530. rRNA_adenine_dimethylase. [Graphical view]
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00755. ksgA. 1 hit.
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KSGA_RICBR

Accession

Primary (citable) accession number: Q1RK29

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	May 16, 2006
Last modified:	November 24, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)