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Q1RK29 (RSMA_RICBR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A
EC=2.1.1.182
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene names
Name:rsmA
Synonyms:ksgA
Ordered Locus Names:RBE_0204
OrganismRickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]
Taxonomic identifier336407 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP MF_00607

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP MF_00607

Subcellular location

Cytoplasm Potential HAMAP MF_00607.

Sequence similarities

Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA (adenine-N6,N6-)-dimethyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 268268Ribosomal RNA small subunit methyltransferase A HAMAP MF_00607
PRO_0000257338

Sites

Binding site231S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site251S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site501S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site721S-adenosyl-L-methionine By similarity
Binding site971S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1RK29 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: 3122E85751E72C9D

FASTA26829,656
        10         20         30         40         50         60 
MLPSIAKHAA SHQIHPLKKH GQNFIFDGSL CDKIVRASGL EENSNVLEIG PGTGGLTRSI 

        70         80         90        100        110        120 
LHKNPKLLTV IETDERCIPL LNEIKQYHPN LNIIKQDALK LKLSDLNTNK ITIISNLPYH 

       130        140        150        160        170        180 
IGTELVIRWL KESSLVASMT LMLQKEVVER ICAKPSTKAY GRLSVICSLI ATVEKCFDVA 

       190        200        210        220        230        240 
PTAFYPPPKV YSAIVKLTPL ENIPNSDLIS KVELITKMAF AGRRKMIKSS LKNLAPNISE 

       250        260 
LLAKLNISDN CRAENLTPND YLSLASLI

« Hide

References

[1]"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RML369-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000087 Genomic DNA. Translation: ABE04285.1.
RefSeqYP_537374.1. NC_007940.1.

3D structure databases

ProteinModelPortalQ1RK29.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ1RK29.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3995968.
GenomeReviewsGene locus RBE_0204 in contig CP000087_GR.
KEGGrbe:RBE_0204.
PATRIC17881705. VBIRicBel102610_0233.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0030.
HOGENOMHBG319664.
OMALPHPAKD.
ProtClustDBPRK00274.

Enzyme and pathway databases

BioCycRBEL336407:RBE_0204-MONOMER.

Family and domain databases

HAMAPMF_00607. 16SrRNA_methyltr_A.
[Tree]
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
[Graphical view]
Gene3DG3DSA:1.10.8.100. rRNA_Ade_diMease-like. 1 hit.
KOK02528.
PANTHERPTHR11727. RRNA_meth_trans. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00755. KsgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMA_RICBR
AccessionPrimary (citable) accession number: Q1RK29
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: May 16, 2006
Last modified: January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia bellii strain RML369-C

Rickettsia bellii (strain RML369-C): entries and gene names

SIMILARITY comments

Index of protein domains and families

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