Reviewed, UniProtKB/Swiss-Prot Q1RJZ5 (SYM_RICBR)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	RBE_0238

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Methionyl-tRNA synthetase HAMAP MF_01228		PRO_0000272401
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_01228
	Motif	295 – 299	5	"KMSKS" region HAMAP MF_01228
Sites
	Binding site	298	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RJZ5-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: FFA102B509977D69	FASTA	509	58,359
	10 20 30 40 50 60 MNNTYYITTP IYYVNDVPHI GHAYTSVASD VIARFMRLSG HEVMFLTGTD EHGQKVEKAA 70 80 90 100 110 120 IDKNIDPQKF TDQTSQSFRH LMTAMHISND DFIRTTEERH KKAVAIFWQK LLDNGSIYEG 130 140 150 160 170 180 FYEGWYAVRD EAFYDESELT ADKLAPTGAA VEWVKEPSYF FNLSKWQDKL LEFYEANPDF 190 200 210 220 230 240 IRPISRRNEV ISFVKSGLKD LSVSRTTFNW GIKVPNDNKH VIYVWLDALA NYISALGYPD 250 260 270 280 290 300 QNSNYGKFWP ANLQVVGKDI LRFHAVYWPA FLMAAEIPLP KTIMAHGWWT NEGQKISKSL 310 320 330 340 350 360 GNTIDPIKLI EEFGVDQVRY FLMREITFGA DGNFARSNLI TRINSELSNK IGNLLQRTTS 370 380 390 400 410 420 FVYKNNDGKV PAITQDAINK IYELPLLKTA INSAKQNILL MEKTEINKIL DNIINLAEEA 430 440 450 460 470 480 NIYIDSEAPW NLKKTDPEKM LEVLYALLET LRYIAVMLQA FMPSSAGKML DQLGVNTEER 490 500 LFKHLSLEFA LTSASDILEP VIVFPRFEE

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04319.1.
RefSeq	YP_537408.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RJZ5.
Genome annotation databases
GeneID	3995386.
GenomeReviews	Gene locus RBE_0238 in contig CP000087_GR.
KEGG	rbe:RBE_0238.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RJZ5.
OMA	DWGIKVP
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0238-MON.
Family and domain databases
HAMAP	MF_01228. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_RICBR

Accession

Primary (citable) accession number: Q1RJZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	May 16, 2006
Last modified:	November 24, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)