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Q1RJZ5 (SYM_RICBR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase

EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:RBE_0238
OrganismRickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]
Taxonomic identifier336407 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 509509Methionine--tRNA ligase HAMAP-Rule MF_01228
PRO_0000272401

Regions

Motif12 – 2211"HIGH" region HAMAP-Rule MF_01228
Motif295 – 2995"KMSKS" region HAMAP-Rule MF_01228

Sites

Binding site2981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1RJZ5 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: FFA102B509977D69

FASTA50958,359
        10         20         30         40         50         60 
MNNTYYITTP IYYVNDVPHI GHAYTSVASD VIARFMRLSG HEVMFLTGTD EHGQKVEKAA 

        70         80         90        100        110        120 
IDKNIDPQKF TDQTSQSFRH LMTAMHISND DFIRTTEERH KKAVAIFWQK LLDNGSIYEG 

       130        140        150        160        170        180 
FYEGWYAVRD EAFYDESELT ADKLAPTGAA VEWVKEPSYF FNLSKWQDKL LEFYEANPDF 

       190        200        210        220        230        240 
IRPISRRNEV ISFVKSGLKD LSVSRTTFNW GIKVPNDNKH VIYVWLDALA NYISALGYPD 

       250        260        270        280        290        300 
QNSNYGKFWP ANLQVVGKDI LRFHAVYWPA FLMAAEIPLP KTIMAHGWWT NEGQKISKSL 

       310        320        330        340        350        360 
GNTIDPIKLI EEFGVDQVRY FLMREITFGA DGNFARSNLI TRINSELSNK IGNLLQRTTS 

       370        380        390        400        410        420 
FVYKNNDGKV PAITQDAINK IYELPLLKTA INSAKQNILL MEKTEINKIL DNIINLAEEA 

       430        440        450        460        470        480 
NIYIDSEAPW NLKKTDPEKM LEVLYALLET LRYIAVMLQA FMPSSAGKML DQLGVNTEER 

       490        500 
LFKHLSLEFA LTSASDILEP VIVFPRFEE 

« Hide

References

[1]"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RML369-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000087 Genomic DNA. Translation: ABE04319.1.
RefSeqYP_537408.1. NC_007940.1.

3D structure databases

ProteinModelPortalQ1RJZ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING336407.RBE_0238.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE04319; ABE04319; RBE_0238.
GeneID3995386.
KEGGrbe:RBE_0238.
PATRIC17881785. VBIRicBel102610_0272.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHOG000200401.
KOK01874.
OMADGKCPDH.
OrthoDBEOG6CVV9B.

Enzyme and pathway databases

BioCycRBEL336407:GJCY-244-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. SSF47323. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYM_RICBR
AccessionPrimary (citable) accession number: Q1RJZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: May 16, 2006
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia bellii strain RML369-C

Rickettsia bellii (strain RML369-C): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries