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Protein

DNA repair protein RadA

Gene

radA

Organism
Rickettsia bellii (strain RML369-C)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 27C4-typeUniRule annotationAdd BLAST18
Nucleotide bindingi91 – 98ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Stress response

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Protein family/group databases

MEROPSiS16.A04.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RadAUniRule annotation (EC:3.6.4.-UniRule annotation)
Alternative name(s):
Branch migration protein RadAUniRule annotation
Gene namesi
Name:radAUniRule annotation
Ordered Locus Names:RBE_0316
OrganismiRickettsia bellii (strain RML369-C)
Taxonomic identifieri336407 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
Proteomesi
  • UP000001951 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002866591 – 448DNA repair protein RadAAdd BLAST448

Structurei

3D structure databases

ProteinModelPortaliQ1RJR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni349 – 448Lon-protease-likeUniRule annotationAdd BLAST100

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi250 – 254RadA KNRFG motifUniRule annotation5

Domaini

Has a putative N-terminal zinc-finger, a middle region with homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.UniRule annotation

Sequence similaritiesi

Belongs to the RecA family. RadA subfamily.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 27C4-typeUniRule annotationAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000218329.
KOiK04485.
OMAiEVGCFEL.
OrthoDBiPOG091H02IA.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1RJR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKEKKHYIC SNCGNTSPKW SGQCFDCGVW GSIIEEKVIS NKNIAKIGSK
60 70 80 90 100
QDFEQLSSNV TDQVRISTPI GELDRVLGGG LVLGSAILIG GDPGIGKSTL
110 120 130 140 150
LLQLVAGSFA SKVKCLYITG EESLDQIKLR ALRLDLVNDK TNILAANNLE
160 170 180 190 200
DIIASLEANK GNIDLVVIDS IQTIATRELT SPPGTVSQIR TCAHELVNYA
210 220 230 240 250
KQNNIIILLS CHVTKDGQLA GPKLLEHLVD TVLYFEGDHN NHFRILRSYK
260 270 280 290 300
NRFGGVGEIG VFEMSSSGLI EVTNPSELFL MKREQNVIGT AIFAGIEGSR
310 320 330 340 350
PLLMEVQALI VPSNMVTPRR SAVGWDVNRL SMILAVLSSR IGLNLANYEV
360 370 380 390 400
YLSIAGGLKI NEPASDLAVA ASLISAATNK PLPEHSVFFG EISLSGEIRK
410 420 430 440
TAKAEARIKE ALKLGFNNII CSKSENLTHD FISSIAHLKD LKLLLGSS
Length:448
Mass (Da):48,450
Last modified:May 16, 2006 - v1
Checksum:iD4F2DDDF1E82C3B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000087 Genomic DNA. Translation: ABE04397.1.
RefSeqiWP_011477008.1. NC_007940.1.

Genome annotation databases

EnsemblBacteriaiABE04397; ABE04397; RBE_0316.
KEGGirbe:RBE_0316.
PATRICi17881953. VBIRicBel102610_0356.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000087 Genomic DNA. Translation: ABE04397.1.
RefSeqiWP_011477008.1. NC_007940.1.

3D structure databases

ProteinModelPortaliQ1RJR7.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS16.A04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE04397; ABE04397; RBE_0316.
KEGGirbe:RBE_0316.
PATRICi17881953. VBIRicBel102610_0356.

Phylogenomic databases

HOGENOMiHOG000218329.
KOiK04485.
OMAiEVGCFEL.
OrthoDBiPOG091H02IA.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRADA_RICBR
AccessioniPrimary (citable) accession number: Q1RJR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 16, 2006
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia bellii strain RML369-C
    Rickettsia bellii (strain RML369-C): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.