Reviewed, UniProtKB/Swiss-Prot Q1RJD8 (TRXB_RICBR)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
      Short name=TRXR
    EC=1.8.1.9

Gene names

Name:	trxB
Ordered Locus Names:	RBE_0445

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Thioredoxin reductase		PRO_0000274790
Regions
	Nucleotide binding	34 – 41	8	FAD By similarity
	Nucleotide binding	281 – 290	10	FAD By similarity
Amino acid modifications
	Disulfide bond	135 ↔ 138		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RJD8-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 950E1F50A5BB9FC7	FASTA	310	33,621
	10 20 30 40 50 60 MKITTKVLII GSGPAGLSAA IYAARASLNP ILINGIQPGG QLTITTDVEN YPGFAESVQG 70 80 90 100 110 120 PWLMEQMRMQ AENVGTKIVN DYVEKVDLSQ RPFKVSTGSR TEYEAESIII CTGAEARWLG 130 140 150 160 170 180 IPTEQEFMGF GVSACATCDG FFFKNQKVVV VGGGNSAVEE ALYLTNHASK VTIVHRRDNF 190 200 210 220 230 240 RAEKILQERL FKNPKISVIW DHVVEEIVGN NNPKSVTGVK IQNVHTKETS LVNCSGVFVA 250 260 270 280 290 300 IGHKPNTALF AEQVTMDNDN YIITTPGSTK TNIEGVFAAG DVQDKIYRQA ITAAGTGCMA 310 ALEAEKFLNK

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04526.1.
RefSeq	YP_537615.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3996237.
GenomeReviews	Gene locus RBE_0445 in contig CP000087_GR.
KEGG	rbe:RBE_0445.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RJD8.
OMA	Q1RJD8. PSCGPCH.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0445-MON.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_RICBR

Accession

Primary (citable) accession number: Q1RJD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)