Reviewed, UniProtKB/Swiss-Prot Q1RJC6 (SYE2_RICBR)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 2
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 2
      Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	RBE_0457

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00022
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Glutamyl-tRNA synthetase 2 HAMAP MF_00022		PRO_0000277972
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RJC6-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 3287F4E1269D22A0	FASTA	464	52,642
	10 20 30 40 50 60 MTNNVITRFA PSPTGFLHIG SARTALFNYL FAKHNNGKFL LRIEDTDKER STEAAVEAIF 70 80 90 100 110 120 SGLKWLGLNW DDEVVFQSKR NDLYKEAALK LLAEGKAYYC FTPQEEIEKQ RQEALENKQH 130 140 150 160 170 180 FIFNSKWRDK TSDTYPKDIK PVIRLKTPSS GSITIHDTLQ GDVVIENCHI DDMVLLRSDG 190 200 210 220 230 240 TATYMLAVVV DDHDMGITHI IRGDDHLTNA ARQIAIYNAF GYHVPIMTHI PLIHGADGAK 250 260 270 280 290 300 LSKRHGALGV EAYKDMGYLP ESLCNYLLRL GWSHGDDEII QMDQAIEWFN LDSLGKSPAR 310 320 330 340 350 360 LDFTKMNSLN SHYLRMLDED SLITKILEIL NRNYKVSEQE VNYIRRGLQG LLVRSETLLD 370 380 390 400 410 420 LAKLAKIYLV NIPVAYESEA KEIIANCDKN LINNVVQGLE KLERFDKESV QDEFKKIAAA 430 440 450 460 NSLKLNEVMK PVRALITGMV GSPSVFEIAE ILGKENILKR LEIK

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04538.1.
RefSeq	YP_537627.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3996031.
GenomeReviews	Gene locus RBE_0457 in contig CP000087_GR.
KEGG	rbe:RBE_0457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RJC6.
OMA	Q1RJC6. IEWFNLD.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0457-MON.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_RICBR

Accession

Primary (citable) accession number: Q1RJC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)