Q1RIS9 (SYFA_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	RBE_0654

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group › Rickettsia bellii

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281		PRO_0000278010
Sites
	Metal binding	258	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RIS9 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: D473EEEE2C2EFFB0	FASTA	349	40,516
	10 20 30 40 50 60 MDNIETILRL AEEKILLVQS LKVLQEYKVE FLGKNGIVTN ELKKLGSLSE QDRKEFGLKI 70 80 90 100 110 120 NKLKEEIQNI IKAKEEILEE EELNLKLSSD KIDLSLPARR YKQGSIHPIT QCMEELIQVF 130 140 150 160 170 180 AKFGFSIEDG PNIENDFHNF TALNFEDDHP ARQMHDTFYL KGQENDKPML LRTHTSTVQI 190 200 210 220 230 240 RAMKNGKPPF RFIAPGRTYR SDSDMTHTPM FHQIEGLVID KDINMGHLKY VITEFIRCFF 250 260 270 280 290 300 ENSNIELRFR PSFFPFTEPS AEVDIRMSKT DKWLEVLGCG MVHPNVLKNV GIDNSQYQGF 310 320 330 340 AFGLGVERFA MLKYNIKDLR QFFEGDMRWL KHYSFSSFDI PNLAGGLTK

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE04735.1.
RefSeq	YP_537824.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RIS9.
ModBase	Search...
Protein-protein interaction databases
STRING	336407.RBE_0654.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE04735; ABE04735; RBE_0654.
GeneID	3996325.
KEGG	rbe:RBE_0654.
PATRIC	17882766. VBIRicBel102610_0750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HOG000242675.
KO	K01889.
OMA	LTHTPMF.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	RBEL336407:GJCY-673-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_ligase_II_N. IPR022911. Phe_tRNA_ligase_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_RICBR

Accession

Primary (citable) accession number: Q1RIS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program