Q1RID9 (GYRA_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	RBE_0794

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	DNA gyrase subunit A HAMAP MF_01897		PRO_0000273107
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RID9 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 6FE9AB2C57BCCC1D	FASTA	905	100,679
	10 20 30 40 50 60 MTDNNSSNLV PVNIEDEMKV SYLDYAMSVI VSRAIPDVRD GLKPVHRRII YSMHEAGNHA 70 80 90 100 110 120 NRAYRKSARI VGDVMGKYHP HGDSAIYDSL VRMAQDFSLR LPLVDGQGNF GSLDGDAAAA 130 140 150 160 170 180 MRYTESRMAK VAHKLIEDID KETVSFNPNY DGSEEEPSVL PSMFPNLLVN GSGGIAVGMA 190 200 210 220 230 240 TNIPPHNLGE VIDACCLYVD NNDIEILDLL EVVKGPDFPT SGIILGVNGI KSAYLTGRGS 250 260 270 280 290 300 IAIRGRAEIE NLGNGRQAII ITEIPYMVNK ARLVEKIAEM VKEKRIEGIS DLRDESNKNG 310 320 330 340 350 360 IRIYIELKKD IVAEVVLNQI YSCTQLQTSF GVIMLALKDG LPKVMNLKEV IAAFVSFREV 370 380 390 400 410 420 VITNRTIYLL NKARDKAHIL LGLTIAVSNI DEIIRIIKAA SDPNAAKQEL MARSWDALNI 430 440 450 460 470 480 LPLVKLVDDK AMLNEEGKCS FTEAQAKAIL EMRLQRLTAM EKNKLEEDLK NLATEITEYL 490 500 510 520 530 540 NILGSRERLL EILKEELIKV KEEFATPRLT SIEFGEFDQD IEDLIQREEM VVTVTLGGYI 550 560 570 580 590 600 KRVPLSSYRA QKRGGKGRSG LSMRDEDITT QVFVGSTHTP MLFFSNIGQV YSLKLYKLPL 610 620 630 640 650 660 SNPQGKGRPM VNILPLKGDE HITNIMPLPE NQDEWDNLNI MFATAKGNIR RSDLLDFKKI 670 680 690 700 710 720 QSNGKIAIRL DEDDKLIDVK PCKEDEHILL ATKSGKALRF PVESLRVIKS RTSDGVRGMR 730 740 750 760 770 780 LAEDDSVISM TVLKGIKATR EERDAYLSVS WEKRLEIAKG EEFNSEELGV DLTAESILEM 790 800 810 820 830 840 ANSEEFILTV TENGFGKRSS AYGYRITDRG GSGIINMDIN DKTGLVVGVM PVKMDDELML 850 860 870 880 890 900 ITNSGKLIRC KLETVRITGR NTSGVTLFRL DDGEKVVSAS LIAESSDDNE EDSEFEEEVA EEGSE

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE04875.1.
RefSeq	YP_537964.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RID9.
SMR	Q1RID9. Positions 31-512.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RID9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3995617.
GenomeReviews	Gene locus RBE_0794 in contig CP000087_GR.
KEGG	rbe:RBE_0794.
PATRIC	17883087. VBIRicBel102610_0907.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0188.
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0794-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_RICBR

Accession

Primary (citable) accession number: Q1RID9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program