Reviewed, UniProtKB/Swiss-Prot Q1RIA5 (UCRI_RICBR)

Names and origin

Protein names

Recommended name:
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    EC=1.10.2.2
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP

Gene names

Name:	petA
Ordered Locus Names:	RBE_0828

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Ubiquinol-cytochrome c reductase iron-sulfur subunit		PRO_0000286641
Regions
	Transmembrane	27 – 47	21	Potential
	Domain	97 – 184	88	Rieske
Sites
	Metal binding	129	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	131	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	148	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	151	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	134 ↔ 150		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RIA5-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: E0E3943826DEF47C	FASTA	186	20,321
	10 20 30 40 50 60 MSDTEDTNND NKVEFEDRSK TSRRDFMVLT ASSVAAVGAA CTLWPLINSL NPSADVLALS 70 80 90 100 110 120 SIEVDLSNIA VGQTVTVKWQ GKPVFITNRT PDKIAEARAV KMSELIDPEE DQDRVKAGHD 130 140 150 160 170 180 NWLVTIGICT HLGCVPLANQ GDYDGWFCPC HGSQYDSSGR VRRGPAPLNL AVPPYTFISD KKIRIG

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE04909.1.
RefSeq	YP_537998.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3995576.
GenomeReviews	Gene locus RBE_0828 in contig CP000087_GR.
KEGG	rbe:RBE_0828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RIA5.
OMA	Q1RIA5. VVGICSH.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_0828-MON.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF10399. UCR_Fe-S_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_RICBR

Accession

Primary (citable) accession number: Q1RIA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)