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Q1RI82 (MFD_RICBR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Transcription-repair-coupling factor
Short name=TRCF
EC=3.6.4.-
Alternative name(s):
ATP-dependent helicase mfd
Gene names
Name:mfd
Ordered Locus Names:RBE_0851
OrganismRickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]
Taxonomic identifier336407 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group ›

Protein attributes

Sequence length1120 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Necessary for strand-specific repair. A lesion in the template strand blocks the RNA polymerase complex (RNAP). The RNAP-DNA-RNA complex is specifically recognized by TRCF which releases RNAP and the truncated transcript; the TCRF may replace RNAP at the lesion site and then recruit the UvrA/B/C repair system By similarity.

Sequence similarities

In the N-terminal section; belongs to the UvrB family.

In the C-terminal section; belongs to the helicase family. RecG subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

damaged DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11201120Transcription-repair-coupling factor
PRO_0000281071

Regions

Domain591 – 756166Helicase ATP-binding
Domain777 – 933157Helicase C-terminal
Nucleotide binding604 – 6118ATP Potential
Motif709 – 7124DEEQ box

Sequences

Sequence LengthMass (Da)Tools
Q1RI82 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: F36F46EEB2A403E5

FASTA1,120127,304
        10         20         30         40         50         60 
MLQQKFPQAA KSFFTIDSFI KNSKQDFVLV TSNEEEALQL YKQALFFLPS ENIYYFPSYD 

        70         80         90        100        110        120 
TIPYDHTSPN CNILSKRAET LSKLTTNKGN KLVITHATNL LNKLPPKDFF AKYYLKLFPK 

       130        140        150        160        170        180 
MKLSANELSK FLVENSFTRN ASTVDVGEFA VRGEIVDLIL PESKGYRINF SWDYVESIKQ 

       190        200        210        220        230        240 
FDIDTQISTR SCNELIISPA NEIVLNPETI SNFKDNYLRN FGVNHTDNPL YEAITGGRKF 

       250        260        270        280        290        300 
SGYEQLLPLF YDSYSGLTDY LNNPVIIFDN LTKQAILEFE HSYNDFYKAR LDANKLKFNS 

       310        320        330        340        350        360 
FYPTLSPSQL YFTSLEAIEL LEQENNILIS YENSEQASIV ENIAAASFVE KKTIFDKLFE 

       370        380        390        400        410        420 
VIKANSRKKI IIGSSVLSSF ERVKSIIENY EYSYNEIEYL EEAKTNTINI AILPLNQSFS 

       430        440        450        460        470        480 
TPEYLFIAAS ELLEEKVTPT NTNKKLKNIL LELDHLAEGE LIVHKDHGIG QFLKLEALEI 

       490        500        510        520        530        540 
KGKLHDFLKI LYAGNDKLYI PVENIEVIKK YGSDVAQLDK LGSVSWQKNK AKLKNRIKEI 

       550        560        570        580        590        600 
ALHLMQIAAK RKLNTTAAIE FDLEEYDKFC AKFPFTETED QLNAINDIRE DLSNGMLMDR 

       610        620        630        640        650        660 
LICGDVGFGK TEVAMRAAFM VAKSLNENSP QVAVVVPTTI LCSQHFARFT ERFKDSDLNI 

       670        680        690        700        710        720 
KQLSSVVSSK EAKIVRSELE SGKINIIIGT HSLLHKVTKF CNLKLLIIDE EQHFGVGQKE 

       730        740        750        760        770        780 
FLKSLKSSTH VLAMSATPIP RTLQMSMTGL KELSIIATPP LNRLEVRTSV MPFDPVIIRD 

       790        800        810        820        830        840 
ALLHEHFRGG KSFFVVPRIN DIEDIEKQLK QIVPELSYKV AHGKMSPNKI DEIMSEFYAG 

       850        860        870        880        890        900 
KFDILISTTI IESGIDIQDA NTMIIHKADM LGLSQLYQLR GRIGRGKMRG YAYLTLPSHK 

       910        920        930        940        950        960 
KMTPHSLRRL EIIQNSCALG SGFTIASHDM DLRGFGNLIG EEQSGQIREV GTELYQEMLE 

       970        980        990       1000       1010       1020 
EQIAIFKDEP ISGEQPFIPT INLGLSVFIP DNYVSDSVLK LGLYRRIGNL NDDLEVEKFK 

      1030       1040       1050       1060       1070       1080 
DEMIDRFGSL PTEFNNLLDI VKIKLLCFKL NIENLDSGDN GFVIKFYKNA DMADKILKFV 

      1090       1100       1110       1120 
STYTANAKIK PDNKLVFIKK LVGKTIITEA NQLLWNLSEI

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References

[1]"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RML369-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000087 Genomic DNA. Translation: ABE04932.1.
RefSeqYP_538021.1. NC_007940.1.

3D structure databases

ProteinModelPortalQ1RI82.
ModBaseSearch...

Protein-protein interaction databases

STRING336407.RBE_0851.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE04932; ABE04932; RBE_0851.
GeneID3995725.
KEGGrbe:RBE_0851.
PATRIC17883209. VBIRicBel102610_0968.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1197.
HOGENOMHOG000216592.
KOK03723.
OMAVIIRDAL.
ProtClustDBCLSK871007.

Enzyme and pathway databases

BioCycRBEL336407:GJCY-873-MONOMER.

Family and domain databases

InterProIPR003711. CarD-like/TRCF_domain.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR004576. Mfd.
IPR005118. Tscpt_repair-coupling_fac_dom.
[Graphical view]
PfamPF02559. CarD_CdnL_TRCF. 1 hit.
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF03461. TRCF. 1 hit.
[Graphical view]
SMARTSM01058. CarD_TRCF. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00982. TRCF. 1 hit.
[Graphical view]
SUPFAMSSF141259. SSF141259. 1 hit.
TIGRFAMsTIGR00580. mfd. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMFD_RICBR
AccessionPrimary (citable) accession number: Q1RI82
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: May 16, 2006
Last modified: May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia bellii strain RML369-C

Rickettsia bellii (strain RML369-C): entries and gene names

SIMILARITY comments

Index of protein domains and families

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