Q1RI04 (DNAB_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replicative DNA helicase
EC=3.6.4.12

Gene names

Name:	dnaB
Ordered Locus Names:	RBE_0929

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group › Rickettsia bellii

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Replicative DNA helicase		PRO_0000281066
Regions
	Domain	197 – 493	297	SF4 helicase
	Nucleotide binding	228 – 235	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RI04 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 6E0500E572F93C08	FASTA	498	56,183
	10 20 30 40 50 60 MARNKTNNDK NIANDEASTV LARVLPSNVQ AEQMLLGAIL TNNELLNYVS EFLRSEHFFE 70 80 90 100 110 120 PIHQKIYNGI EVITEKGLIA TPITLRSMLA NDELFHEMEG AEYLAKLVTM SMMVINPIDY 130 140 150 160 170 180 GKIIYDLAIK RYLINIGEEV VNDAYNSSLE FEAKEQIERA ESKLYDLASE GINEKSFTKI 190 200 210 220 230 240 AIPISESIAS INRAMKNNDH VIGISTGLID LDSKLFGFHN SDLVILAGRP AMGKTAFALN 250 260 270 280 290 300 LALNACNNML IKNKRDNEEV QSVGFFSLEM SSEQLTTRLL SMSAGIDSTT LRTGMINEAN 310 320 330 340 350 360 YNRLRKEAIA LSELSFFIDD TPALTISAIR TRARRMKRKH NLGILFVDYL QLIRGVSKSD 370 380 390 400 410 420 NRVNEVSEIT QGLKAIAKEL NIPVIALSQL SRAVELREDK RPMLSDLRES GSIEQDADIV 430 440 450 460 470 480 MFIYREEYYL SRKEPAASDP KHAEKYAEWQ DNMSKAHNIT DIIIAKHRSG PVGNVQLFYD 490 SQYSKFVNLE TKYPVHPR

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE05010.1.
RefSeq	YP_538099.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RI04.
ModBase	Search...
Protein-protein interaction databases
STRING	336407.RBE_0929.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE05010; ABE05010; RBE_0929.
GeneID	3994940.
KEGG	rbe:RBE_0929.
PATRIC	17883391. VBIRicBel102610_1056.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0305.
HOGENOM	HOG000113195.
KO	K02314.
OMA	KDYTQIN.
ProtClustDB	PRK09165.
Enzyme and pathway databases
BioCyc	RBEL336407:GJCY-954-MONOMER.
Family and domain databases
Gene3D	1.10.860.10. 1 hit.
InterPro	IPR007692. DNA_helicase_DnaB. IPR007694. DNA_helicase_DnaB-like_C. IPR007693. DNA_helicase_DnaB-like_N. IPR016136. DNA_helicase_N/primase_C. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00772. DnaB. 1 hit. PF03796. DnaB_C. 1 hit. [Graphical view]
SUPFAM	SSF48024. DNA_helicase_DnaB-like_N. 1 hit. SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00665. DnaB. 1 hit.
PROSITE	PS51199. SF4_HELICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAB_RICBR

Accession

Primary (citable) accession number: Q1RI04

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	May 16, 2006
Last modified:	May 29, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program