Q1RHI9 (UVRB_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UvrABC system protein B
Short name=Protein UvrB

Alternative name(s):
Excinuclease ABC subunit B

Gene names

Name:	uvrB
Ordered Locus Names:	RBE_1094

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group › Rickettsia bellii

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204
Subunit structure	Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204
Sequence similarities	Belongs to the UvrB family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 UVR domain.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Excision nuclease Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	SOS response Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC nucleotide-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP excinuclease ABC activity Inferred from electronic annotation. Source: HAMAP helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 661	661	UvrABC system protein B HAMAP-Rule MF_00204		PRO_0000277986
Regions
	Domain	25 – 182	158	Helicase ATP-binding
	Domain	430 – 592	163	Helicase C-terminal
	Domain	621 – 656	36	UVR
	Nucleotide binding	38 – 45	8	ATP By similarity
	Motif	91 – 114	24	Beta-hairpin HAMAP-Rule MF_00204

Sequences

	Sequence		Length	Mass (Da)
	Q1RHI9 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: D4446CCF7424E0AB	FASTA	661	75,518
	10 20 30 40 50 60 MSNFSIISDY KPAGDQPKAI DEIIKGLNNK KRSQMLLGIT GSGKTFTMAN IIERTNRPTL 70 80 90 100 110 120 IMAHNKTLAA QIYSEMKSIF PKNAVEYFVS YYDYYQPEAY IPKTDVFIEK DSSINEQIDL 130 140 150 160 170 180 MRHSATRSLL ERRDVIVVSS VSCIYGLGSP DLYYQMTVNL EPGKSYPRDK LLNDLVNLQY 190 200 210 220 230 240 ERNDIGFERG CFRVKGDNVD VFPSHYSDKA WRLSFFGNEL EYIHEFDPLT GEKLVKLDKA 250 260 270 280 290 300 IIYGNSHFVM PKETVNKAIS EIEEELQKRI AFLKSQDKLL ETQRINQRTQ YDLEMLTETG 310 320 330 340 350 360 SCKGVENYSR FFTGRKAGEP PPTLFEYLPK DALLFVDESH VSVPQIRAMY NGDRARKEVL 370 380 390 400 410 420 VEHGFRLPSA LDNRPLKFEE WDNFRPQTVF VSATPSLFEL NETGGEVVEL IIRPTGLLDP 430 440 450 460 470 480 ECIIKPATNQ VEDLVGEIQS TIAKGFCVLV TTLTKKMAED LTNYLQELKY KTSYLHSNIH 490 500 510 520 530 540 TLERIEILRD LRQGTIDILV GINLLREGLD IPECGLVAIL DADKEGFLRS EVSLIQTIGR 550 560 570 580 590 600 AARNSEGRVI LYADKMTKSI DKAISETMRR RTIQQEHNEK YGIIPKTINR TIHALAELEK 610 620 630 640 650 660 VDSKLDKKQT HNLFENPAKL KAHIDKLRKE MLKAASNLEF EQAAKLRDQL KTLEEAALEL S

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE05175.1.
RefSeq	YP_538264.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RHI9.
SMR	Q1RHI9. Positions 4-592.
ModBase	Search...
Protein-protein interaction databases
STRING	336407.RBE_1094.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE05175; ABE05175; RBE_1094.
GeneID	3995005.
KEGG	rbe:RBE_1094.
PATRIC	17883765. VBIRicBel102610_1235.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0556.
HOGENOM	HOG000073580.
KO	K03702.
OMA	CIYGLGI.
ProtClustDB	PRK05298.
Enzyme and pathway databases
BioCyc	RBEL336407:GJCY-1127-MONOMER.
Family and domain databases
Gene3D	4.10.860.10. 1 hit.
HAMAP	MF_00204. UvrB.
InterPro	IPR006935. Helicase/UvrB_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR001943. UVR_dom. IPR004807. UvrB. IPR024759. UvrB_YAD/RRR_dom. [Graphical view]
PANTHER	PTHR24029. PTHR24029. 1 hit.
Pfam	PF00271. Helicase_C. 1 hit. PF04851. ResIII. 1 hit. PF02151. UVR. 1 hit. PF12344. UvrB. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF46600. UvrB_C. 1 hit.
TIGRFAMs	TIGR00631. uvrb. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50151. UVR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UVRB_RICBR

Accession

Primary (citable) accession number: Q1RHI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program