Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q1RHI9 (UVRB_RICBR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UvrABC system protein B

Short name=Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene names
Name:uvrB
Ordered Locus Names:RBE_1094
OrganismRickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]
Taxonomic identifier336407 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group

Protein attributes

Sequence length661 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 UVR domain.

Ontologies

Keywords
   Biological processDNA damage
DNA excision
DNA repair
SOS response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionExcision nuclease
Helicase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processSOS response

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleotide-excision repair

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

excinuclease ABC activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

helicase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 661661UvrABC system protein B HAMAP-Rule MF_00204
PRO_0000277986

Regions

Domain25 – 182158Helicase ATP-binding
Domain430 – 592163Helicase C-terminal
Domain621 – 65636UVR
Nucleotide binding38 – 458ATP By similarity
Motif91 – 11424Beta-hairpin HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
Q1RHI9 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: D4446CCF7424E0AB

FASTA66175,518
        10         20         30         40         50         60 
MSNFSIISDY KPAGDQPKAI DEIIKGLNNK KRSQMLLGIT GSGKTFTMAN IIERTNRPTL 

        70         80         90        100        110        120 
IMAHNKTLAA QIYSEMKSIF PKNAVEYFVS YYDYYQPEAY IPKTDVFIEK DSSINEQIDL 

       130        140        150        160        170        180 
MRHSATRSLL ERRDVIVVSS VSCIYGLGSP DLYYQMTVNL EPGKSYPRDK LLNDLVNLQY 

       190        200        210        220        230        240 
ERNDIGFERG CFRVKGDNVD VFPSHYSDKA WRLSFFGNEL EYIHEFDPLT GEKLVKLDKA 

       250        260        270        280        290        300 
IIYGNSHFVM PKETVNKAIS EIEEELQKRI AFLKSQDKLL ETQRINQRTQ YDLEMLTETG 

       310        320        330        340        350        360 
SCKGVENYSR FFTGRKAGEP PPTLFEYLPK DALLFVDESH VSVPQIRAMY NGDRARKEVL 

       370        380        390        400        410        420 
VEHGFRLPSA LDNRPLKFEE WDNFRPQTVF VSATPSLFEL NETGGEVVEL IIRPTGLLDP 

       430        440        450        460        470        480 
ECIIKPATNQ VEDLVGEIQS TIAKGFCVLV TTLTKKMAED LTNYLQELKY KTSYLHSNIH 

       490        500        510        520        530        540 
TLERIEILRD LRQGTIDILV GINLLREGLD IPECGLVAIL DADKEGFLRS EVSLIQTIGR 

       550        560        570        580        590        600 
AARNSEGRVI LYADKMTKSI DKAISETMRR RTIQQEHNEK YGIIPKTINR TIHALAELEK 

       610        620        630        640        650        660 
VDSKLDKKQT HNLFENPAKL KAHIDKLRKE MLKAASNLEF EQAAKLRDQL KTLEEAALEL 


S 

« Hide

References

[1]"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RML369-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000087 Genomic DNA. Translation: ABE05175.1.
RefSeqYP_538264.1. NC_007940.1.

3D structure databases

ProteinModelPortalQ1RHI9.
SMRQ1RHI9. Positions 4-592.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING336407.RBE_1094.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE05175; ABE05175; RBE_1094.
GeneID3995005.
KEGGrbe:RBE_1094.
PATRIC17883765. VBIRicBel102610_1235.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0556.
HOGENOMHOG000073580.
KOK03702.
OMAMQYERND.
OrthoDBEOG6B360R.
ProtClustDBPRK05298.

Enzyme and pathway databases

BioCycRBEL336407:GJCY-1127-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUVRB_RICBR
AccessionPrimary (citable) accession number: Q1RHI9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 16, 2006
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia bellii strain RML369-C

Rickettsia bellii (strain RML369-C): entries and gene names