Reviewed, UniProtKB/Swiss-Prot Q1RHB9 (DHSA_RICBR)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase flavoprotein subunit
    EC=1.3.99.1

Gene names

Name:	sdhA
Ordered Locus Names:	RBE_1164

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Succinate dehydrogenase flavoprotein subunit		PRO_0000280976
Regions
	Nucleotide binding	41 – 56	16	FAD By similarity
Sites
	Active site	290	1	Proton acceptor By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	258	1	Substrate By similarity
	Binding site	357	1	Substrate By similarity
	Binding site	402	1	Substrate By similarity
Amino acid modifications
	Modified residue	49	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RHB9-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: F3A2C76895400AF4	FASTA	596	65,106
	10 20 30 40 50 60 MTKSYNIIHH KFDVIVVGAG GAGLRAAFGM AEEGLNTACI SKLFPTRSHT VAAQGGISAA 70 80 90 100 110 120 LGNMGADDWR WHMYDTVKGS DWLGDQDAIE YMCKNAPDAI LELEHYGVPF SRTEEGKIYQ 130 140 150 160 170 180 RPFGGMTTEY GKGKAAQRTC AAADRTGHAI LHTLYQQSLK HKVQFFIEYF AIDLLMENGE 190 200 210 220 230 240 CRGVVAWNLD DGSLHCFRAH NVVLATGGYG RAYFSATSAH TCTGDGGGMA IRAGLPLQDM 250 260 270 280 290 300 EFVQFHPTGI YSAGCLITEG ARGEGGYLVN ANGERFMERY APAAKDLASR DVVSRAMTIE 310 320 330 340 350 360 IREGRGVGEH KDHVFLHLNH LSPEIVHSRL PGISETAKIF AGVDVTKEPI PVLPTVHYNM 370 380 390 400 410 420 GGIPTNYHGQ VIIKDGKNHN SVVKGLMSIG EAACVSVHGA NRLGSNSLLD LVVFGRSAAL 430 440 450 460 470 480 KAAELIKPAS PHKPVSEEAL EKIISRFDKI RHSSGNISVA DLRLKMQRTM QSHASVFRTQ 490 500 510 520 530 540 EVLDEGAEMI SEIRSGYKDI KVNDKSLIWN SDLVEALELD NLLDQALVTV YSAAARKESR 550 560 570 580 590 GAHAREDYPD RNDKEWMQHT LSGVDEAGKV VLDYKPVTLT TLSDEVKAIP PAKRVY

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE05245.1.
RefSeq	YP_538334.1.
3D structure databases
SMR	Q1RHB9. Positions 5-596.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RHB9.
Genome annotation databases
GeneID	3995942.
GenomeReviews	Gene locus RBE_1164 in contig CP000087_GR.
KEGG	rbe:RBE_1164.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1053.
HOGENOM	HBG293998.
OMA	VYELEHW.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_1164-MONOMER.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_RICBR

Accession

Primary (citable) accession number: Q1RHB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	May 16, 2006
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)