Reviewed, UniProtKB/Swiss-Prot Q1RHA1 (LEP_RICBR)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	RBE_1182

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Signal peptidase I		PRO_0000316273
Regions
	Topological domain	1 – 45	45	Cytoplasmic Potential
	Transmembrane	46 – 66	21	Potential
	Topological domain	67 – 291	225	Periplasmic Potential
Sites
	Active site	70	1	By similarity
	Active site	133	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RHA1-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 0C743E8E8A94B434	FASTA	291	34,121
	10 20 30 40 50 60 MTMKKLTSTT TTLWDNKLFI NNLKNFMQTN TESNNNKTTA QEWKSFILVV VIALMIRILI 70 80 90 100 110 120 IESFVVPTGS MKATILENDR IFGTKYSYGY SNYSLSFFDF IHLFKGRIFA RTPERGDIII 130 140 150 160 170 180 FRPPHEMNTR YIKRLIGLPG DKVQLIDDVI YINDEKIERV ESGIYVSEEG RKYLKFKETL 190 200 210 220 230 240 PNGKTYFSYK LAPVLGIMFN DKYGNTDAFY VPEGEYFFLG DNRDQSNDSR IDLGFVPFEN 250 260 270 280 290 FIAKAQFIWF STKITWWDSD IGVINLILKL KPWAESIRFN RIFRNLYSIE D

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE05263.1.
RefSeq	YP_538352.1.
3D structure databases
SMR	Q1RHA1. Positions 57-253.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RHA1.
Protein family/group databases
MEROPS	S26.001.
Genome annotation databases
GeneID	3996354.
GenomeReviews	Gene locus RBE_1182 in contig CP000087_GR.
KEGG	rbe:RBE_1182.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0681.
HOGENOM	HBG596607.
OMA	EISESSH.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_1182-MONOMER.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_RICBR

Accession

Primary (citable) accession number: Q1RHA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 16, 2006
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)