Q1RHA0 (NUOF_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit F
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	RBE_1183

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	NADH-quinone oxidoreductase subunit F		PRO_0000274787
Regions
	Nucleotide binding	54 – 63	10	NAD By similarity
	Nucleotide binding	166 – 213	48	FMN By similarity
Sites
	Metal binding	345	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	348	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	351	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	391	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RHA0 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: AD1855FFF50107C9	FASTA	417	45,632
	10 20 30 40 50 60 MLKKEDRIFT NLHGEQSHDL KSSKKLGDWD NTKALLNKGK EWIIEEVKKS GLRGRGGAGF 70 80 90 100 110 120 STGTKWSFMP KESKKPSYLV VNADESEPGT CKDRDILRYE PHKLIEGCLL ASFAIGANSC 130 140 150 160 170 180 YIYIRGEFYN EASNIQRALD EAYKDGLIGK NACGSGFNCD IYLHRGAGAY ICGEETALLE 190 200 210 220 230 240 SLEGKKGMPR LKPPFPAGFG LYGCPTTINN VESIAVVPTI LRRGASWFAS IGKPNNTGTK 250 260 270 280 290 300 IFCISGHVNK PCNVEEAMGI PLKEIIEKHA GGVRGGWDNL KAIIPGGSSV PLLPKSLCET 310 320 330 340 350 360 ADMDFDSLKA AGSSLGTGGI IVMDKSTDII YAIARLSKFY MHESCGQCTP CREGTGWMWR 370 380 390 400 410 VMMRLVKGDA KKSEIDQLLE VTKEIEGHTI CALGDAAAWP IQGLIKHFRN EIESRIQ

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE05264.1.
RefSeq	YP_538353.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RHA0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1RHA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3996355.
GenomeReviews	Gene locus RBE_1183 in contig CP000087_GR.
KEGG	rbe:RBE_1183.
PATRIC	17883971. VBIRicBel102610_1336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1894.
HOGENOM	HBG669338.
OMA	AGLNGDN.
ProtClustDB	PRK13596.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_1183-MONOMER.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51kDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51kDa_su. IPR019554. Soluble_ligand-bd. [Graphical view]
KO	K00335.
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
SMART	SM00928. NADH_4Fe-4S. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. NuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_RICBR

Accession

Primary (citable) accession number: Q1RHA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program