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Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia bellii (strain RML369-C)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171S-adenosyl-L-methionineBy similarity1
Binding sitei200S-adenosyl-L-methionineBy similarity1
Binding sitei215S-adenosyl-L-methionineBy similarity1
Binding sitei403S-adenosyl-L-methionineBy similarity1
Binding sitei428S-adenosyl-L-methionineBy similarity1
Binding sitei455S-adenosyl-L-methionineBy similarity1
Active sitei477By similarity1
Binding sitei477S-adenosyl-L-methionineBy similarity1
Binding sitei481SubstrateBy similarity1
Binding sitei513SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RBE_1243
OrganismiRickettsia bellii (strain RML369-C)
Taxonomic identifieri336407 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
Proteomesi
  • UP000001951 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002884341 – 556Bifunctional methyltransferaseAdd BLAST556

Structurei

3D structure databases

ProteinModelPortaliQ1RH40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 310RF MTaseAdd BLAST310
Regioni148 – 152S-adenosyl-L-methionine bindingBy similarity5
Regioni215 – 218Substrate bindingBy similarity4
Regioni313 – 556tRNA MTaseAdd BLAST244
Regioni348 – 399InsertAdd BLAST52

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000010896.
KOiK02493.
OMAiDDISENC.
OrthoDBiPOG091H02CJ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
MF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR022438. RPE5.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR03776. RPE5. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1RH40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYSIQKFLN EGAYKLQHIG INNPKLEARI LLQHAINKPY EYLLANPEKQ
60 70 80 90 100
LNQLEIEAVE KVLERRLKHE PIAYILGTKE FYSREFIVNK HVLIPRNDTE
110 120 130 140 150
ILIDVVLQYH SQHSLCHSSN GGNPDKKQLD SVVKPRNNIK SSNILELGTG
160 170 180 190 200
SGCISISLLL ELPNSQITAT DISIDAIEVA KSNAIKHDVT DRLQIIHSNW
210 220 230 240 250
FENIGKQKFD LIVSNPPYIS INEKPEMAIE TINYEPSIAL FAEEDGLLSY
260 270 280 290 300
KIIAENAKKF LKQNGKIILE IGYKQADQVS QIFLDHGYVI DNIHQDLQSH
310 320 330 340 350
NRVIEISLIQ LNRSYARRIG KSLSGIQQNL LDNELPKYLF SKEKLIGKNY
360 370 380 390 400
NSCKIKSNYT KFNLEKSKES VSRGAERIKI REHLRTYKED VANFSSSTSI
410 420 430 440 450
FLEIGFGMGE HFINQAKMNP DKLFIGVEVY LNGVANVLKL AEEQNITNFL
460 470 480 490 500
LFPNNLDFIL HDLPNNSLDR IYILFPDPWI KNRQKKKRIL NKERLTILQT
510 520 530 540 550
KLKNKGSLIF TSDIENYFEE VVELIKQNGN FQITNEDNYS KPHDNYIITK

IPPKSY
Length:556
Mass (Da):63,993
Last modified:May 16, 2006 - v1
Checksum:iEA14666271E6FE1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000087 Genomic DNA. Translation: ABE05324.1.
RefSeqiWP_011477898.1. NC_007940.1.

Genome annotation databases

EnsemblBacteriaiABE05324; ABE05324; RBE_1243.
KEGGirbe:RBE_1243.
PATRICi17884110. VBIRicBel102610_1404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000087 Genomic DNA. Translation: ABE05324.1.
RefSeqiWP_011477898.1. NC_007940.1.

3D structure databases

ProteinModelPortaliQ1RH40.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE05324; ABE05324; RBE_1243.
KEGGirbe:RBE_1243.
PATRICi17884110. VBIRicBel102610_1404.

Phylogenomic databases

HOGENOMiHOG000010896.
KOiK02493.
OMAiDDISENC.
OrthoDBiPOG091H02CJ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
MF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR022438. RPE5.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR03776. RPE5. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFTRM_RICBR
AccessioniPrimary (citable) accession number: Q1RH40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 16, 2006
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia bellii strain RML369-C
    Rickettsia bellii (strain RML369-C): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.