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Reviewed, UniProtKB/Swiss-Prot Q1RH40 (HEMK_RICBR)

Last modified June 16, 2009. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Bifunctional methyltransferase
Including the following 2 domains:
    1- Recommended name:
            HemK protein homolog
              EC=2.1.1.-
    2- Recommended name:
            tRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.33
        Alternative name(s):
            tRNA(m7G46)-methyltransferase
Gene names
Name: hemK
Ordered Locus Names: RBE_1243
OrganismRickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]
Taxonomic identifier336407 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsia

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.

Catalytic activity

S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057

Sequence similarities

In the N-terminal section; belongs to the hemK family.

In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Bifunctional methyltransferase HAMAP MF_01057
PRO_0000288434

Regions

Region348 – 39952Insert HAMAP MF_01057

Sites

Binding site4031S-adenosyl-L-methionine By similarity
Binding site4281S-adenosyl-L-methionine By similarity
Binding site4551S-adenosyl-L-methionine By similarity
Binding site4771S-adenosyl-L-methionine By similarity
Binding site4811Substrate By similarity
Binding site5131Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q1RH40-1 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: EA14666271E6FE1E

FASTA55663,993
        10         20         30         40         50         60 
MQYSIQKFLN EGAYKLQHIG INNPKLEARI LLQHAINKPY EYLLANPEKQ LNQLEIEAVE 

        70         80         90        100        110        120 
KVLERRLKHE PIAYILGTKE FYSREFIVNK HVLIPRNDTE ILIDVVLQYH SQHSLCHSSN 

       130        140        150        160        170        180 
GGNPDKKQLD SVVKPRNNIK SSNILELGTG SGCISISLLL ELPNSQITAT DISIDAIEVA 

       190        200        210        220        230        240 
KSNAIKHDVT DRLQIIHSNW FENIGKQKFD LIVSNPPYIS INEKPEMAIE TINYEPSIAL 

       250        260        270        280        290        300 
FAEEDGLLSY KIIAENAKKF LKQNGKIILE IGYKQADQVS QIFLDHGYVI DNIHQDLQSH 

       310        320        330        340        350        360 
NRVIEISLIQ LNRSYARRIG KSLSGIQQNL LDNELPKYLF SKEKLIGKNY NSCKIKSNYT 

       370        380        390        400        410        420 
KFNLEKSKES VSRGAERIKI REHLRTYKED VANFSSSTSI FLEIGFGMGE HFINQAKMNP 

       430        440        450        460        470        480 
DKLFIGVEVY LNGVANVLKL AEEQNITNFL LFPNNLDFIL HDLPNNSLDR IYILFPDPWI 

       490        500        510        520        530        540 
KNRQKKKRIL NKERLTILQT KLKNKGSLIF TSDIENYFEE VVELIKQNGN FQITNEDNYS 

       550 
KPHDNYIITK IPPKSY 

« Hide

References

[1]"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

CP000087 Genomic DNA. Translation: ABE05324.1.
RefSeqYP_538413.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3996337.
GenomeReviewsGene locus RBE_1243 in contig CP000087_GR.
KEGGrbe:RBE_1243.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ1RH40.
OMAQ1RH40. IGVEVYL.

Enzyme and pathway databases

BioCycRBEL336407:RBE_1243-MON.

Family and domain databases

HAMAPMF_01057. Fused.
[Tree]
InterProIPR004556. Modification_methylase_HemK.
IPR002052. N6_adenine_Mtase_CS.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR007848. Small_mtfrase.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_HemK. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit. Uncertain.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEMK_RICBR
AccessionPrimary (citable) accession number: Q1RH40
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 16, 2006
Last modified: June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Rickettsia bellii strain RML369-C

Rickettsia bellii (strain RML369-C): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents