Reviewed, UniProtKB/Swiss-Prot Q1RH40 (HEMK_RICBR)

Names and origin

Protein names

Recommended name:
    Bifunctional methyltransferase
Including the following 2 domains:
    1- Recommended name:
            HemK protein homolog
              EC=2.1.1.-
    2- Recommended name:
            tRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.33
        Alternative name(s):
            tRNA(m7G46)-methyltransferase

Gene names

Name:	hemK
Ordered Locus Names:	RBE_1243

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Sequence similarities	In the N-terminal section; belongs to the hemK family. In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid methylation Inferred from electronic annotation. Source: InterPro tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro protein methyltransferase activity Inferred from electronic annotation. Source: InterPro tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Bifunctional methyltransferase HAMAP MF_01057		PRO_0000288434
Regions
	Region	348 – 399	52	Insert HAMAP MF_01057
Sites
	Binding site	403	1	S-adenosyl-L-methionine By similarity
	Binding site	428	1	S-adenosyl-L-methionine By similarity
	Binding site	455	1	S-adenosyl-L-methionine By similarity
	Binding site	477	1	S-adenosyl-L-methionine By similarity
	Binding site	481	1	Substrate By similarity
	Binding site	513	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1RH40-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: EA14666271E6FE1E	FASTA	556	63,993
	10 20 30 40 50 60 MQYSIQKFLN EGAYKLQHIG INNPKLEARI LLQHAINKPY EYLLANPEKQ LNQLEIEAVE 70 80 90 100 110 120 KVLERRLKHE PIAYILGTKE FYSREFIVNK HVLIPRNDTE ILIDVVLQYH SQHSLCHSSN 130 140 150 160 170 180 GGNPDKKQLD SVVKPRNNIK SSNILELGTG SGCISISLLL ELPNSQITAT DISIDAIEVA 190 200 210 220 230 240 KSNAIKHDVT DRLQIIHSNW FENIGKQKFD LIVSNPPYIS INEKPEMAIE TINYEPSIAL 250 260 270 280 290 300 FAEEDGLLSY KIIAENAKKF LKQNGKIILE IGYKQADQVS QIFLDHGYVI DNIHQDLQSH 310 320 330 340 350 360 NRVIEISLIQ LNRSYARRIG KSLSGIQQNL LDNELPKYLF SKEKLIGKNY NSCKIKSNYT 370 380 390 400 410 420 KFNLEKSKES VSRGAERIKI REHLRTYKED VANFSSSTSI FLEIGFGMGE HFINQAKMNP 430 440 450 460 470 480 DKLFIGVEVY LNGVANVLKL AEEQNITNFL LFPNNLDFIL HDLPNNSLDR IYILFPDPWI 490 500 510 520 530 540 KNRQKKKRIL NKERLTILQT KLKNKGSLIF TSDIENYFEE VVELIKQNGN FQITNEDNYS 550 KPHDNYIITK IPPKSY

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed: 16703114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000087 Genomic DNA. Translation: ABE05324.1.
RefSeq	YP_538413.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3996337.
GenomeReviews	Gene locus RBE_1243 in contig CP000087_GR.
KEGG	rbe:RBE_1243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1RH40.
OMA	Q1RH40. IGVEVYL.
Enzyme and pathway databases
BioCyc	RBEL336407:RBE_1243-MON.
Family and domain databases
HAMAP	MF_01057. Fused. [Tree]
InterPro	IPR004556. Modification_methylase_HemK. IPR002052. N6_adenine_Mtase_CS. IPR019874. Release_fac_Glu-N5_MeTfrase. IPR007848. Small_mtfrase. IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. PF05175. MTS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00536. hemK_fam. 1 hit. TIGR03534. RF_mod_HemK. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMK_RICBR

Accession

Primary (citable) accession number: Q1RH40

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)