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Q1RH40 (RFTRM_RICBR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional methyltransferase

Including the following 2 domains:

  1. Release factor glutamine methyltransferase
    Short name=RF MTase
    EC=2.1.1.297
    Alternative name(s):
    N5-glutamine methyltransferase PrmC
    Protein-(glutamine-N5) MTase PrmC
    Protein-glutamine N-methyltransferase PrmC
  2. tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
Gene names
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RBE_1243
OrganismRickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]
Taxonomic identifier336407 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif By similarity. HAMAP-Rule MF_01057

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine. HAMAP-Rule MF_01057

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Sequence similarities

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Bifunctional methyltransferase HAMAP-Rule MF_01057
PRO_0000288434

Regions

Region1 – 310310RF MTase HAMAP-Rule MF_01057
Region148 – 1525S-adenosyl-L-methionine binding By similarity
Region215 – 2184Substrate binding By similarity
Region313 – 556244tRNA MTase HAMAP-Rule MF_01057
Region348 – 39952Insert HAMAP-Rule MF_01057

Sites

Active site4771 By similarity
Binding site1711S-adenosyl-L-methionine By similarity
Binding site2001S-adenosyl-L-methionine By similarity
Binding site2151S-adenosyl-L-methionine By similarity
Binding site4031S-adenosyl-L-methionine By similarity
Binding site4281S-adenosyl-L-methionine By similarity
Binding site4551S-adenosyl-L-methionine By similarity
Binding site4771S-adenosyl-L-methionine By similarity
Binding site4811Substrate By similarity
Binding site5131Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1RH40 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: EA14666271E6FE1E

FASTA55663,993
        10         20         30         40         50         60 
MQYSIQKFLN EGAYKLQHIG INNPKLEARI LLQHAINKPY EYLLANPEKQ LNQLEIEAVE 

        70         80         90        100        110        120 
KVLERRLKHE PIAYILGTKE FYSREFIVNK HVLIPRNDTE ILIDVVLQYH SQHSLCHSSN 

       130        140        150        160        170        180 
GGNPDKKQLD SVVKPRNNIK SSNILELGTG SGCISISLLL ELPNSQITAT DISIDAIEVA 

       190        200        210        220        230        240 
KSNAIKHDVT DRLQIIHSNW FENIGKQKFD LIVSNPPYIS INEKPEMAIE TINYEPSIAL 

       250        260        270        280        290        300 
FAEEDGLLSY KIIAENAKKF LKQNGKIILE IGYKQADQVS QIFLDHGYVI DNIHQDLQSH 

       310        320        330        340        350        360 
NRVIEISLIQ LNRSYARRIG KSLSGIQQNL LDNELPKYLF SKEKLIGKNY NSCKIKSNYT 

       370        380        390        400        410        420 
KFNLEKSKES VSRGAERIKI REHLRTYKED VANFSSSTSI FLEIGFGMGE HFINQAKMNP 

       430        440        450        460        470        480 
DKLFIGVEVY LNGVANVLKL AEEQNITNFL LFPNNLDFIL HDLPNNSLDR IYILFPDPWI 

       490        500        510        520        530        540 
KNRQKKKRIL NKERLTILQT KLKNKGSLIF TSDIENYFEE VVELIKQNGN FQITNEDNYS 

       550 
KPHDNYIITK IPPKSY 

« Hide

References

[1]"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RML369-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000087 Genomic DNA. Translation: ABE05324.1.
RefSeqYP_538413.1. NC_007940.1.

3D structure databases

ProteinModelPortalQ1RH40.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING336407.RBE_1243.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE05324; ABE05324; RBE_1243.
GeneID3996337.
KEGGrbe:RBE_1243.
PATRIC17884110. VBIRicBel102610_1404.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000010896.
KOK02493.
OMAQQNLLDN.
OrthoDBEOG68Q0SZ.
ProtClustDBPRK01544.

Enzyme and pathway databases

BioCycRBEL336407:GJCY-1280-MONOMER.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR022438. RPE5.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR03776. RPE5. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFTRM_RICBR
AccessionPrimary (citable) accession number: Q1RH40
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 16, 2006
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia bellii strain RML369-C

Rickettsia bellii (strain RML369-C): entries and gene names