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Q1RH40

- RFTRM_RICBR

UniProt

Q1RH40 - RFTRM_RICBR

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Protein

Bifunctional methyltransferase

Gene
prmC/trmB, hemK, RBE_1243
Organism
Rickettsia bellii (strain RML369-C)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif By similarity.UniRule annotation
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.UniRule annotation
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711S-adenosyl-L-methionine By similarity
Binding sitei200 – 2001S-adenosyl-L-methionine By similarity
Binding sitei215 – 2151S-adenosyl-L-methionine By similarity
Binding sitei403 – 4031S-adenosyl-L-methionine By similarity
Binding sitei428 – 4281S-adenosyl-L-methionine By similarity
Binding sitei455 – 4551S-adenosyl-L-methionine By similarity
Active sitei477 – 4771 By similarity
Binding sitei477 – 4771S-adenosyl-L-methionine By similarity
Binding sitei481 – 4811Substrate By similarity
Binding sitei513 – 5131Substrate By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. protein methyltransferase activity Source: InterPro
  3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRBEL336407:GJCY-1280-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RBE_1243
OrganismiRickettsia bellii (strain RML369-C)
Taxonomic identifieri336407 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
ProteomesiUP000001951: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Bifunctional methyltransferaseUniRule annotationPRO_0000288434Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi336407.RBE_1243.

Structurei

3D structure databases

ProteinModelPortaliQ1RH40.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 310310RF MTaseUniRule annotationAdd
BLAST
Regioni148 – 1525S-adenosyl-L-methionine binding By similarity
Regioni215 – 2184Substrate binding By similarity
Regioni313 – 556244tRNA MTaseUniRule annotationAdd
BLAST
Regioni348 – 39952InsertUniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000010896.
KOiK02493.
OMAiQQNLLDN.
OrthoDBiEOG68Q0SZ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR022438. RPE5.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR03776. RPE5. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1RH40-1 [UniParc]FASTAAdd to Basket

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MQYSIQKFLN EGAYKLQHIG INNPKLEARI LLQHAINKPY EYLLANPEKQ    50
LNQLEIEAVE KVLERRLKHE PIAYILGTKE FYSREFIVNK HVLIPRNDTE 100
ILIDVVLQYH SQHSLCHSSN GGNPDKKQLD SVVKPRNNIK SSNILELGTG 150
SGCISISLLL ELPNSQITAT DISIDAIEVA KSNAIKHDVT DRLQIIHSNW 200
FENIGKQKFD LIVSNPPYIS INEKPEMAIE TINYEPSIAL FAEEDGLLSY 250
KIIAENAKKF LKQNGKIILE IGYKQADQVS QIFLDHGYVI DNIHQDLQSH 300
NRVIEISLIQ LNRSYARRIG KSLSGIQQNL LDNELPKYLF SKEKLIGKNY 350
NSCKIKSNYT KFNLEKSKES VSRGAERIKI REHLRTYKED VANFSSSTSI 400
FLEIGFGMGE HFINQAKMNP DKLFIGVEVY LNGVANVLKL AEEQNITNFL 450
LFPNNLDFIL HDLPNNSLDR IYILFPDPWI KNRQKKKRIL NKERLTILQT 500
KLKNKGSLIF TSDIENYFEE VVELIKQNGN FQITNEDNYS KPHDNYIITK 550
IPPKSY 556
Length:556
Mass (Da):63,993
Last modified:May 16, 2006 - v1
Checksum:iEA14666271E6FE1E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000087 Genomic DNA. Translation: ABE05324.1.
RefSeqiYP_538413.1. NC_007940.1.

Genome annotation databases

EnsemblBacteriaiABE05324; ABE05324; RBE_1243.
GeneIDi3996337.
KEGGirbe:RBE_1243.
PATRICi17884110. VBIRicBel102610_1404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000087 Genomic DNA. Translation: ABE05324.1 .
RefSeqi YP_538413.1. NC_007940.1.

3D structure databases

ProteinModelPortali Q1RH40.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 336407.RBE_1243.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE05324 ; ABE05324 ; RBE_1243 .
GeneIDi 3996337.
KEGGi rbe:RBE_1243.
PATRICi 17884110. VBIRicBel102610_1404.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000010896.
KOi K02493.
OMAi QQNLLDN.
OrthoDBi EOG68Q0SZ.

Enzyme and pathway databases

BioCyci RBEL336407:GJCY-1280-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR022438. RPE5.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 3 hits.
TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR03776. RPE5. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
    Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
    PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RML369-C.

Entry informationi

Entry nameiRFTRM_RICBR
AccessioniPrimary (citable) accession number: Q1RH40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 16, 2006
Last modified: June 11, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia bellii strain RML369-C
    Rickettsia bellii (strain RML369-C): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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