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Q1RH40

- RFTRM_RICBR

UniProt

Q1RH40 - RFTRM_RICBR

Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia bellii (strain RML369-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (16 May 2006)
      Previous versions | rss
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    Functioni

    Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei171 – 1711S-adenosyl-L-methionineBy similarity
    Binding sitei200 – 2001S-adenosyl-L-methionineBy similarity
    Binding sitei215 – 2151S-adenosyl-L-methionineBy similarity
    Binding sitei403 – 4031S-adenosyl-L-methionineBy similarity
    Binding sitei428 – 4281S-adenosyl-L-methionineBy similarity
    Binding sitei455 – 4551S-adenosyl-L-methionineBy similarity
    Active sitei477 – 4771By similarity
    Binding sitei477 – 4771S-adenosyl-L-methionineBy similarity
    Binding sitei481 – 4811SubstrateBy similarity
    Binding sitei513 – 5131SubstrateBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. protein methyltransferase activity Source: InterPro
    3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRBEL336407:GJCY-1280-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional methyltransferase
    Including the following 2 domains:
    Release factor glutamine methyltransferase (EC:2.1.1.297)
    Short name:
    RF MTase
    Alternative name(s):
    N5-glutamine methyltransferase PrmC
    Protein-(glutamine-N5) MTase PrmC
    Protein-glutamine N-methyltransferase PrmC
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:prmC/trmB
    Synonyms:hemK
    Ordered Locus Names:RBE_1243
    OrganismiRickettsia bellii (strain RML369-C)
    Taxonomic identifieri336407 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
    ProteomesiUP000001951: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 556556Bifunctional methyltransferasePRO_0000288434Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi336407.RBE_1243.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1RH40.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 310310RF MTaseAdd
    BLAST
    Regioni148 – 1525S-adenosyl-L-methionine bindingBy similarity
    Regioni215 – 2184Substrate bindingBy similarity
    Regioni313 – 556244tRNA MTaseAdd
    BLAST
    Regioni348 – 39952InsertAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
    In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000010896.
    KOiK02493.
    OMAiQQNLLDN.
    OrthoDBiEOG68Q0SZ.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR022438. RPE5.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 3 hits.
    TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR03776. RPE5. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1RH40-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQYSIQKFLN EGAYKLQHIG INNPKLEARI LLQHAINKPY EYLLANPEKQ    50
    LNQLEIEAVE KVLERRLKHE PIAYILGTKE FYSREFIVNK HVLIPRNDTE 100
    ILIDVVLQYH SQHSLCHSSN GGNPDKKQLD SVVKPRNNIK SSNILELGTG 150
    SGCISISLLL ELPNSQITAT DISIDAIEVA KSNAIKHDVT DRLQIIHSNW 200
    FENIGKQKFD LIVSNPPYIS INEKPEMAIE TINYEPSIAL FAEEDGLLSY 250
    KIIAENAKKF LKQNGKIILE IGYKQADQVS QIFLDHGYVI DNIHQDLQSH 300
    NRVIEISLIQ LNRSYARRIG KSLSGIQQNL LDNELPKYLF SKEKLIGKNY 350
    NSCKIKSNYT KFNLEKSKES VSRGAERIKI REHLRTYKED VANFSSSTSI 400
    FLEIGFGMGE HFINQAKMNP DKLFIGVEVY LNGVANVLKL AEEQNITNFL 450
    LFPNNLDFIL HDLPNNSLDR IYILFPDPWI KNRQKKKRIL NKERLTILQT 500
    KLKNKGSLIF TSDIENYFEE VVELIKQNGN FQITNEDNYS KPHDNYIITK 550
    IPPKSY 556
    Length:556
    Mass (Da):63,993
    Last modified:May 16, 2006 - v1
    Checksum:iEA14666271E6FE1E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000087 Genomic DNA. Translation: ABE05324.1.
    RefSeqiYP_538413.1. NC_007940.1.

    Genome annotation databases

    EnsemblBacteriaiABE05324; ABE05324; RBE_1243.
    GeneIDi3996337.
    KEGGirbe:RBE_1243.
    PATRICi17884110. VBIRicBel102610_1404.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000087 Genomic DNA. Translation: ABE05324.1 .
    RefSeqi YP_538413.1. NC_007940.1.

    3D structure databases

    ProteinModelPortali Q1RH40.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 336407.RBE_1243.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE05324 ; ABE05324 ; RBE_1243 .
    GeneIDi 3996337.
    KEGGi rbe:RBE_1243.
    PATRICi 17884110. VBIRicBel102610_1404.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000010896.
    KOi K02493.
    OMAi QQNLLDN.
    OrthoDBi EOG68Q0SZ.

    Enzyme and pathway databases

    BioCyci RBEL336407:GJCY-1280-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR022438. RPE5.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 3 hits.
    TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR03776. RPE5. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
      Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
      PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RML369-C.

    Entry informationi

    Entry nameiRFTRM_RICBR
    AccessioniPrimary (citable) accession number: Q1RH40
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia bellii strain RML369-C
      Rickettsia bellii (strain RML369-C): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3