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Q1RGY9 (QUEF_RICBR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase

EC=1.7.1.13
Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase
Gene names
Name:queF
Ordered Locus Names:RBE_1294
OrganismRickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]
Taxonomic identifier336407 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00817

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00817.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionoxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor

Inferred from electronic annotation. Source: InterPro

preQ1 synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817
PRO_0000247717

Regions

Nucleotide binding83 – 842NADPH By similarity
Nucleotide binding247 – 2482NADPH By similarity
Region81 – 833Substrate binding By similarity
Region218 – 2192Substrate binding By similarity

Sites

Active site1791Thioimide intermediate By similarity
Active site1861Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1RGY9 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: 70548EE9DDC193D3

FASTA27331,146
        10         20         30         40         50         60 
MTLSNSALGK KSTYKDTYDP TLLFKIPRID NRKELGIVND KLPFHGVDIW NAYELSWLDK 

        70         80         90        100        110        120 
KGKPCVAIFT FFVPTTSSHI VESKSVKLYL NSFNNFVVDS MEELKRTILQ DLSNNTHAEV 

       130        140        150        160        170        180 
TGEIFPINTK IEFGSPTGIN IDDIDIECSE YGPPNNSLIK HEDVLVEEEL NSNLLKSNCL 

       190        200        210        220        230        240 
VTGQPDWGTI IIKYKGKKLK HDALLKYLVS FRNCNEFAEQ CAERIFTDIK NAINPEFLSI 

       250        260        270 
YIIYTRRGGI DICPYRSTDS NYTLPSSKRL IRQ 

« Hide

References

[1]"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RML369-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000087 Genomic DNA. Translation: ABE05375.1.
RefSeqYP_538464.1. NC_007940.1.

3D structure databases

ProteinModelPortalQ1RGY9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING336407.RBE_1294.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE05375; ABE05375; RBE_1294.
GeneID3995983.
KEGGrbe:RBE_1294.
PATRIC17884222. VBIRicBel102610_1459.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0780.
HOGENOMHOG000273755.
KOK06879.
OMAIFCDLMH.
OrthoDBEOG6D5G0M.

Enzyme and pathway databases

BioCycRBEL336407:GJCY-1332-MONOMER.
UniPathwayUPA00392.

Family and domain databases

HAMAPMF_00817. QueF_type2.
InterProIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsTIGR03138. QueF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_RICBR
AccessionPrimary (citable) accession number: Q1RGY9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: May 16, 2006
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia bellii strain RML369-C

Rickettsia bellii (strain RML369-C): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways