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Protein

Endonuclease III

Gene

nth

Organism
Rickettsia bellii (strain RML369-C)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi289 – 2891Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi296 – 2961Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi299 – 2991Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi305 – 3051Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  3. DNA binding Source: UniProtKB-HAMAP
  4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. base-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseImported, GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotationImported, Nuclease

Keywords - Biological processi

DNA damage, DNA repairUniRule annotation

Keywords - Ligandi

4Fe-4SUniRule annotation, DNA-bindingUniRule annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciRBEL336407:GJCY-1344-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotationImported
Ordered Locus Names:RBE_1306Imported
OrganismiRickettsia bellii (strain RML369-C)Imported
Taxonomic identifieri336407 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
ProteomesiUP000001951: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi336407.RBE_1306.

Structurei

3D structure databases

ProteinModelPortaliQ1RGX7.
SMRiQ1RGX7. Positions 106-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 22928HhHUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation
Contains 1 HhH domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0177.
HOGENOMiHOG000252206.
KOiK10773.
OrthoDBiEOG6H4KC5.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1RGX7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQPPTNSPL IYNCGNVGQS LKTFKDSRNS GSDNTSISRN STPSLVKIIT
60 70 80 90 100
TLPENPHIGA SLVPFMNNTT LLFFISFSIN LKLLFSNIKN LICLILFVTL
110 120 130 140 150
YTMQAEIVNK IFEVFSKNNE NPKTELVYKN NFTLLVAVIL SAQATDVSVN
160 170 180 190 200
LATKSLFEIY DTPEKILGLG EEGLKKYIKS IGLFNSKAKN VIALCQILIS
210 220 230 240 250
NYDSKVPNNF EELVKLPGVG RKTANVVLNC LFGLPTMAVD THVFRVAKRI
260 270 280 290 300
GLAKGNTPEA VEKELLQIID GKWLSHAHHW LILHGRYICK ARKPDCEICP
310
IKEYCEYYKS ATKII
Length:315
Mass (Da):35,162
Last modified:May 16, 2006 - v1
Checksum:i03575C9BCD220E9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000087 Genomic DNA. Translation: ABE05387.1.
RefSeqiWP_011477957.1. NC_007940.1.
YP_538476.1. NC_007940.1.

Genome annotation databases

EnsemblBacteriaiABE05387; ABE05387; RBE_1306.
GeneIDi3995523.
KEGGirbe:RBE_1306.
PATRICi17884246. VBIRicBel102610_1471.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000087 Genomic DNA. Translation: ABE05387.1.
RefSeqiWP_011477957.1. NC_007940.1.
YP_538476.1. NC_007940.1.

3D structure databases

ProteinModelPortaliQ1RGX7.
SMRiQ1RGX7. Positions 106-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi336407.RBE_1306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE05387; ABE05387; RBE_1306.
GeneIDi3995523.
KEGGirbe:RBE_1306.
PATRICi17884246. VBIRicBel102610_1471.

Phylogenomic databases

eggNOGiCOG0177.
HOGENOMiHOG000252206.
KOiK10773.
OrthoDBiEOG6H4KC5.

Enzyme and pathway databases

BioCyciRBEL336407:GJCY-1344-MONOMER.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
    Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
    PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RML369-CImported.

Entry informationi

Entry nameiQ1RGX7_RICBR
AccessioniPrimary (citable) accession number: Q1RGX7
Entry historyi
Integrated into UniProtKB/TrEMBL: May 16, 2006
Last sequence update: May 16, 2006
Last modified: January 7, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.