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Q1RGX4 (LIPA_RICBR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase

EC=2.8.1.8
Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene names
Name:lipA
Ordered Locus Names:RBE_1309
OrganismRickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]
Taxonomic identifier336407 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Contains 1 RPE1 insert domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Lipoyl synthase HAMAP-Rule MF_00206
PRO_0000278007

Regions

Domain7 – 5549RPE1 insert

Sites

Metal binding861Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding911Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding971Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1121Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1161Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1191Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1RGX4 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: 9AAB44B06E00AE89

FASTA35539,989
        10         20         30         40         50         60 
MTNLDRHLSK FAYREEFAGN TEVLATAAYK EDCADASTGL TPKLPLEVEF GKMSKRPDWI 

        70         80         90        100        110        120 
KVKAPNSSEY YNTKDLIKNL KLNTVCEEAA CPNIGECWSK KHATVMILGS VCTRACRFCN 

       130        140        150        160        170        180 
VKTGRPDLLD PHEPQRLAEA VQKLGLKHVV ITSVDRDDLE DGGATHFAEC ISEIRKSSPN 

       190        200        210        220        230        240 
TTIEILTPDF LRKDGAAEII ANAKPDVFNH NVETVPSLYN TIRPGARYYN SLSLLHNIKK 

       250        260        270        280        290        300 
LSPEVFTKSG MMVGLGEEIS EVVQVMDDLR EAKVDFLTIG QYLQPTKNHA EVAKYVTPEE 

       310        320        330        340        350 
FKYLERVART KGFLMVSASP LTRSSYHADE DFEKLKENYR HRHCEERRSI DVAIS 

« Hide

References

[1]"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens."
Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D.
PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RML369-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000087 Genomic DNA. Translation: ABE05390.1.
RefSeqYP_538479.1. NC_007940.1.

3D structure databases

ProteinModelPortalQ1RGX4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING336407.RBE_1309.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE05390; ABE05390; RBE_1309.
GeneID3995526.
KEGGrbe:RBE_1309.
PATRIC17884254. VBIRicBel102610_1475.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0181.
HOGENOMHOG000235998.
KOK03644.
OMAHPHIPTK.
OrthoDBEOG6038ZS.

Enzyme and pathway databases

BioCycRBEL336407:GJCY-1347-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR005728. Rickett_RPE.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
TIGR01045. RPE1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA_RICBR
AccessionPrimary (citable) accession number: Q1RGX4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 16, 2006
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia bellii strain RML369-C

Rickettsia bellii (strain RML369-C): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways