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Protein

Lipoyl synthase

Gene

lipA

Organism
Rickettsia bellii (strain RML369-C)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi86Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi91Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi97Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi112Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi116Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi119Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:RBE_1309
OrganismiRickettsia bellii (strain RML369-C)
Taxonomic identifieri336407 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
Proteomesi
  • UP000001951 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002780071 – 355Lipoyl synthaseAdd BLAST355

Proteomic databases

PRIDEiQ1RGX4

Structurei

3D structure databases

ProteinModelPortaliQ1RGX4
SMRiQ1RGX4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 55RPE1 insertAdd BLAST49

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000235998
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG091H069D

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR005728 Rickett_RPE
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit
TIGR01045 RPE1, 1 hit

Sequencei

Sequence statusi: Complete.

Q1RGX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNLDRHLSK FAYREEFAGN TEVLATAAYK EDCADASTGL TPKLPLEVEF
60 70 80 90 100
GKMSKRPDWI KVKAPNSSEY YNTKDLIKNL KLNTVCEEAA CPNIGECWSK
110 120 130 140 150
KHATVMILGS VCTRACRFCN VKTGRPDLLD PHEPQRLAEA VQKLGLKHVV
160 170 180 190 200
ITSVDRDDLE DGGATHFAEC ISEIRKSSPN TTIEILTPDF LRKDGAAEII
210 220 230 240 250
ANAKPDVFNH NVETVPSLYN TIRPGARYYN SLSLLHNIKK LSPEVFTKSG
260 270 280 290 300
MMVGLGEEIS EVVQVMDDLR EAKVDFLTIG QYLQPTKNHA EVAKYVTPEE
310 320 330 340 350
FKYLERVART KGFLMVSASP LTRSSYHADE DFEKLKENYR HRHCEERRSI

DVAIS
Length:355
Mass (Da):39,989
Last modified:May 16, 2006 - v1
Checksum:i9AAB44B06E00AE89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000087 Genomic DNA Translation: ABE05390.1

Genome annotation databases

EnsemblBacteriaiABE05390; ABE05390; RBE_1309
KEGGirbe:RBE_1309

Similar proteinsi

Entry informationi

Entry nameiLIPA_RICBR
AccessioniPrimary (citable) accession number: Q1RGX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 16, 2006
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia bellii strain RML369-C
    Rickettsia bellii (strain RML369-C): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

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