Q1RGX4 (LIPA_RICBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase
EC=2.8.1.8

Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA

Gene names

Name:	lipA
Ordered Locus Names:	RBE_1309

Organism

Rickettsia bellii (strain RML369-C) [Complete proteome] [HAMAP]

Taxonomic identifier

336407 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group › Rickettsia bellii

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00206.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Lipoyl synthase HAMAP-Rule MF_00206		PRO_0000278007
Regions
	Domain	7 – 55	49	RPE1 insert
Sites
	Metal binding	86	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	91	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	97	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	112	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	116	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	119	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1RGX4 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 9AAB44B06E00AE89	FASTA	355	39,989
	10 20 30 40 50 60 MTNLDRHLSK FAYREEFAGN TEVLATAAYK EDCADASTGL TPKLPLEVEF GKMSKRPDWI 70 80 90 100 110 120 KVKAPNSSEY YNTKDLIKNL KLNTVCEEAA CPNIGECWSK KHATVMILGS VCTRACRFCN 130 140 150 160 170 180 VKTGRPDLLD PHEPQRLAEA VQKLGLKHVV ITSVDRDDLE DGGATHFAEC ISEIRKSSPN 190 200 210 220 230 240 TTIEILTPDF LRKDGAAEII ANAKPDVFNH NVETVPSLYN TIRPGARYYN SLSLLHNIKK 250 260 270 280 290 300 LSPEVFTKSG MMVGLGEEIS EVVQVMDDLR EAKVDFLTIG QYLQPTKNHA EVAKYVTPEE 310 320 330 340 350 FKYLERVART KGFLMVSASP LTRSSYHADE DFEKLKENYR HRHCEERRSI DVAIS

References

[1]

"Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens." Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C., Fournier P.-E., Claverie J.-M., Raoult D. PLoS Genet. 2:733-744(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RML369-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000087 Genomic DNA. Translation: ABE05390.1.
RefSeq	YP_538479.1. NC_007940.1.
3D structure databases
ProteinModelPortal	Q1RGX4.
ModBase	Search...
Protein-protein interaction databases
STRING	336407.RBE_1309.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE05390; ABE05390; RBE_1309.
GeneID	3995526.
KEGG	rbe:RBE_1309.
PATRIC	17884254. VBIRicBel102610_1475.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0181.
HOGENOM	HOG000235998.
KO	K03644.
OMA	DPHEPQR.
ProtClustDB	PRK05481.
Enzyme and pathway databases
BioCyc	RBEL336407:GJCY-1347-MONOMER.
UniPathway	UPA00538; UER00593.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth. Divergent sequence.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR005728. Rickett_RPE. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit. TIGR01045. RPE1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIPA_RICBR

Accession

Primary (citable) accession number: Q1RGX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program